Talarico, L.B.; Batalle, J.P.; Byrne, A.B.; Brahamian, J.M.; Ferretti, A.; García, A.G.; Mauri, A.; Simonetto, C.; Hijano, D.R.; Lawrence, A.; Acosta, P.L.; Caballero, M.T.; Paredes Rojas, Y.; Ibañez, L.I.; Melendi, G.A.; Rey, F.A.; Damonte, E.B.; Harris, E.; Polack, F.P."The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection" (2017) EBioMedicine. 20:202-216
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Dengue is the most prevalent arthropod-borne viral disease worldwide and is caused by the four dengue virus serotypes (DENV-1-4). Sequential heterologous DENV infections can be associated with severe disease manifestations. Here, we present an immunocompetent mouse model of secondary DENV infection using non mouse-adapted DENV strains to investigate the pathogenesis of severe dengue disease. C57BL/6 mice infected sequentially with DENV-1 (strain Puerto Rico/94) and DENV-2 (strain Tonga/74) developed low platelet counts, internal hemorrhages, and increase of liver enzymes. Cross-reactive CD8+ T lymphocytes were found to be necessary and sufficient for signs of severe disease by adoptively transferring of DENV-1-immune CD8+ T lymphocytes before DENV-2 challenge. Disease signs were associated with production of tumor necrosis factor (TNF)-α and elevated cytotoxicity displayed by heterotypic anti-DENV-1 CD8+ T lymphocytes. These findings highlight the critical role of heterotypic anti-DENV CD8+ T lymphocytes in manifestations of severe dengue disease. © 2017 The Authors


Documento: Artículo
Título:The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection
Autor:Talarico, L.B.; Batalle, J.P.; Byrne, A.B.; Brahamian, J.M.; Ferretti, A.; García, A.G.; Mauri, A.; Simonetto, C.; Hijano, D.R.; Lawrence, A.; Acosta, P.L.; Caballero, M.T.; Paredes Rojas, Y.; Ibañez, L.I.; Melendi, G.A.; Rey, F.A.; Damonte, E.B.; Harris, E.; Polack, F.P.
Filiación:Fundación INFANT, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Department of Pediatrics, Vaccine Center, Vanderbilt University, Nashville, TN, United States
Centro de Virología Animal (CEVAN), Instituto de Ciencia y Tecnología Dr. César Milstein, CONICET, Buenos Aires, Argentina
Département de Virologie, Institut Pasteur, Unité de Virologie Structurale, Paris, France
Laboratorio de Virología, Departamento de Química Biológica-IQUIBICEN (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Division of Infectious Diseases and Vaccinology, School of Public Health, University of California Berkeley, Berkeley, CA, United States
Palabras clave:CD8+ T lymphocytes; Dengue virus; Immune response; Immunocompetent mouse model; Secondary infection; enrofloxacin; granzyme B; ketamine; tramadol; xylazine; immunoglobulin G; neutralizing antibody; T lymphocyte receptor; virus antibody; adoptive transfer; Aedes albopictus; alanine aminotransferase blood level; alkaline phosphatase blood level; animal cell; animal model; animal tissue; antibody response; antisepsis; Article; aspartate aminotransferase blood level; bleeding time; blood vessel permeability; CD8+ T lymphocyte; controlled study; creatine kinase blood level; dengue; Dengue virus 1; Dengue virus 2; depilation; female; flow cytometry; histopathology; Human respiratory syncytial virus; humoral immune deficiency; immunoassay; lactate dehydrogenase blood level; male; mouse; nonhuman; priority journal; real time polymerase chain reaction; RNA extraction; T cell depletion; thrombocyte count; ultraviolet irradiation; virus neutralization; virus titration; wound healing; animal; CD8+ T lymphocyte; cell line; classification; cross reaction; dengue; Dengue virus; disease model; immunology; knockout mouse; lymphocyte depletion; metabolism; serotype; severity of illness index; T lymphocyte subpopulation; virology; virus load; Animals; Antibodies, Neutralizing; Antibodies, Viral; CD8-Positive T-Lymphocytes; Cell Line; Cross Reactions; Dengue; Dengue Virus; Disease Models, Animal; Immunoglobulin G; Lymphocyte Depletion; Mice; Mice, Knockout; Serogroup; Severity of Illness Index; T-Cell Antigen Receptor Specificity; T-Lymphocyte Subsets; Viral Load
Página de inicio:202
Página de fin:216
Título revista:EBioMedicine
Título revista abreviado:EBioMedicine
CAS:enrofloxacin, 93106-60-6; granzyme B; ketamine, 1867-66-9, 6740-88-1, 81771-21-3; tramadol, 27203-92-5, 36282-47-0; xylazine, 23076-35-9, 7361-61-7; immunoglobulin G, 97794-27-9; Antibodies, Neutralizing; Antibodies, Viral; Immunoglobulin G


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---------- APA ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., Mauri, A.,..., Polack, F.P. (2017) . The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection. EBioMedicine, 20, 202-216.
---------- CHICAGO ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., et al. "The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection" . EBioMedicine 20 (2017) : 202-216.
---------- MLA ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., et al. "The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection" . EBioMedicine, vol. 20, 2017, pp. 202-216.
---------- VANCOUVER ----------
Talarico, L.B., Batalle, J.P., Byrne, A.B., Brahamian, J.M., Ferretti, A., García, A.G., et al. The Role of Heterotypic DENV-specific CD8+ T Lymphocytes in an Immunocompetent Mouse Model of Secondary Dengue Virus Infection. EBioMedicine. 2017;20:202-216.