Artículo

Martinez, M.D.C.; Ruspini, S.F.; Afonso, S.G.; Meiss, R.; Buzaleh, A.M.; Batlle, A. "Experimental protoporphyria: Effect of bile acids on liver damage induced by griseofulvin" (2015) BioMed Research International. 2015
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Abstract:

The effect of bile acids administration to an experimental mice model of Protoporphyria produced by griseofulvin (Gris) was investigated. The aim was to assess whether porphyrin excretion could be accelerated by bile acids treatment in an attempt to diminish liver damage induced by Gris. Liver damage markers, heme metabolism, and oxidative stress parameters were analyzed in mice treated with Gris and deoxycholic (DXA), dehydrocholic (DHA), chenodeoxycholic, or ursodeoxycholic (URSO). The administration of Gris alone increased the activities of glutathione reductase (GRed), superoxide dismutase (SOD), alkaline phosphatase (AP), gamma glutamyl transpeptidase (GGT), and glutathione-S-transferase (GST), as well as total porphyrins, glutathione (GSH), and cytochrome P450 (CYP) levels in liver. Among the bile acids studied, DXA and DHA increased PROTO IX excretion, DXA also abolished the action of Gris, reducing lipid peroxidation and hepatic GSH and CYP levels, and the activities of GGT, AP, SOD, and GST returned to control values. However, porphyrin accumulation was not prevented by URSO; instead this bile acid reduced ALA-S and the antioxidant defense enzymes system activities. In conclusion, we postulate that DXA acid would be more effective to prevent liver damage induced by Gris. © 2015 Mariá del Carmen Martinez et al.

Registro:

Documento: Artículo
Título:Experimental protoporphyria: Effect of bile acids on liver damage induced by griseofulvin
Autor:Martinez, M.D.C.; Ruspini, S.F.; Afonso, S.G.; Meiss, R.; Buzaleh, A.M.; Batlle, A.
Filiación:Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Guiraldes 2160, Buenos Aires, 1428, Argentina
Centro de Investigaciones Sobre Porfirinas y Porfirias, CONICET-UBA, Avenida Cordoba 2351, Buenos Aires, 1120, Argentina
Departamento de Patologia, Instituto de Estudios Oncologicos, Academia Nacional de Medicina, Pacheco de Melo 3081, Buenos Aires, 1425, Argentina
Palabras clave:alkaline phosphatase; biological marker; chenodeoxycholic acid; cytochrome P450; dehydrocholic acid; deoxycholic acid; gamma glutamyltransferase; glutathione; glutathione reductase; glutathione transferase; griseofulvin; heme; porphyrin; protoporphyrin; superoxide dismutase; ursodeoxycholic acid; catalase; chenodeoxycholic acid; dehydrocholic acid; deoxycholic acid; glutathione peroxidase; glutathione reductase; glutathione transferase; griseofulvin; porphyrin; superoxide dismutase; ursodeoxycholic acid; animal experiment; animal model; animal tissue; Article; biliary excretion; controlled study; drug effect; drug efficacy; enzyme activity; erythropoietic protoporphyria; immunohistochemistry; lipid peroxidation; liver histology; liver injury; male; mouse; nonhuman; oxidative stress; animal; Chemical and Drug Induced Liver Injury; chemically induced; drug effects; human; metabolism; pathology; Protoporphyria, Erythropoietic; Mus; Animals; Catalase; Chemical and Drug Induced Liver Injury; Chenodeoxycholic Acid; Dehydrocholic Acid; Deoxycholic Acid; Glutathione Peroxidase; Glutathione Reductase; Glutathione Transferase; Griseofulvin; Humans; Lipid Peroxidation; Mice; Oxidative Stress; Porphyrins; Protoporphyria, Erythropoietic; Superoxide Dismutase; Ursodeoxycholic Acid
Año:2015
Volumen:2015
DOI: http://dx.doi.org/10.1155/2015/436319
Título revista:BioMed Research International
Título revista abreviado:BioMed Res. Int.
ISSN:23146133
CAS:alkaline phosphatase, 9001-78-9; chenodeoxycholic acid, 474-25-9; cytochrome P450, 9035-51-2; dehydrocholic acid, 145-41-5, 81-23-2; deoxycholic acid, 83-44-3; gamma glutamyltransferase, 85876-02-4; glutathione, 70-18-8; glutathione reductase, 9001-48-3; glutathione transferase, 50812-37-8; griseofulvin, 126-07-8; heme, 14875-96-8; porphyrin, 24869-67-8; protoporphyrin, 553-12-8; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; ursodeoxycholic acid, 128-13-2, 2898-95-5; catalase, 9001-05-2; glutathione peroxidase, 9013-66-5; Catalase; Chenodeoxycholic Acid; Dehydrocholic Acid; Deoxycholic Acid; Glutathione Peroxidase; Glutathione Reductase; Glutathione Transferase; Griseofulvin; Porphyrins; Superoxide Dismutase; Ursodeoxycholic Acid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2015_n_p_Martinez

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Citas:

---------- APA ----------
Martinez, M.D.C., Ruspini, S.F., Afonso, S.G., Meiss, R., Buzaleh, A.M. & Batlle, A. (2015) . Experimental protoporphyria: Effect of bile acids on liver damage induced by griseofulvin. BioMed Research International, 2015.
http://dx.doi.org/10.1155/2015/436319
---------- CHICAGO ----------
Martinez, M.D.C., Ruspini, S.F., Afonso, S.G., Meiss, R., Buzaleh, A.M., Batlle, A. "Experimental protoporphyria: Effect of bile acids on liver damage induced by griseofulvin" . BioMed Research International 2015 (2015).
http://dx.doi.org/10.1155/2015/436319
---------- MLA ----------
Martinez, M.D.C., Ruspini, S.F., Afonso, S.G., Meiss, R., Buzaleh, A.M., Batlle, A. "Experimental protoporphyria: Effect of bile acids on liver damage induced by griseofulvin" . BioMed Research International, vol. 2015, 2015.
http://dx.doi.org/10.1155/2015/436319
---------- VANCOUVER ----------
Martinez, M.D.C., Ruspini, S.F., Afonso, S.G., Meiss, R., Buzaleh, A.M., Batlle, A. Experimental protoporphyria: Effect of bile acids on liver damage induced by griseofulvin. BioMed Res. Int. 2015;2015.
http://dx.doi.org/10.1155/2015/436319