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Abstract:

Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues. Both compounds reduce the incidence and severity of lesions in a murine model of herpetic stromal keratitis when administered in different treatment modalities. Since encephalitis caused by HSV-1 is another immunopathology of viral origin, we evaluate here the antiviral effect of both compounds on HSV-1 infected nervous cell lines as well as their anti-inflammatory action. We found that both stigmasterol derivatives presented low cytotoxicity in the three nervous cell lines assayed. Regarding the antiviral activity, in all cases both compounds prevented HSV-1 multiplication when added after infection, as well as virus propagation. Additionally, both compounds were able to hinder interleukin-6 and Interferon-gamma secretion induced by HSV-1 infection in Neuro-2a cells. We conclude that compounds 1 and 2 have exerted a dual antiviral and anti-inflammatory effect in HSV-1 infected nervous cell lines, which makes them interesting molecules to be further studied. © 2014 Erina Petrera et al.

Registro:

Documento: Artículo
Título:Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro
Autor:Petrera, E.; Níttolo, A.G.; Alché, L.E.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 4to. Piso, Buenos Aires, C1428EGA, Argentina
Palabras clave:22,23 dihydroxystigmast 4 en 3 one; 3beta bromo 5alpha,22,23 trihydroxystigmastan 6 one; gamma interferon; interleukin 6; stigmasterol; unclassified drug; 22,23-dihydroxystigmast-4-en-3-one; 3-bromo-5,22,23-trihydroxystigmastan-6-one; antiinflammatory agent; antivirus agent; cholestane derivative; gamma interferon; interleukin 6; stigmasterol; animal cell; antiinflammatory activity; antiviral activity; Article; controlled study; cytokine production; cytotoxicity assay; herpes simplex keratitis; Herpes simplex virus 1; human; human cell; immunopathology; interferon production; nerve cell; nonhuman; virus inhibition; virus replication; analogs and derivatives; animal; cell line; Chlorocebus aethiops; drug effects; epithelium cell; Herpes simplex virus 1; inflammation; metabolism; mouse; Vero cell line; virus replication; Human herpesvirus 1; Murinae; Simplexvirus; Animals; Anti-Inflammatory Agents; Antiviral Agents; Cell Line; Cercopithecus aethiops; Cholestanones; Epithelial Cells; Herpesvirus 1, Human; Humans; Inflammation; Interferon-gamma; Interleukin-6; Mice; Stigmasterol; Vero Cells; Virus Replication
Año:2014
Volumen:2014
DOI: http://dx.doi.org/10.1155/2014/947560
Título revista:BioMed Research International
Título revista abreviado:BioMed Res. Int.
ISSN:23146133
CAS:gamma interferon, 82115-62-6; stigmasterol, 83-48-7; 22,23-dihydroxystigmast-4-en-3-one; 3-bromo-5,22,23-trihydroxystigmastan-6-one; Anti-Inflammatory Agents; Antiviral Agents; Cholestanones; Interferon-gamma; Interleukin-6; Stigmasterol
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_23146133_v2014_n_p_Petrera

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Citas:

---------- APA ----------
Petrera, E., Níttolo, A.G. & Alché, L.E. (2014) . Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro. BioMed Research International, 2014.
http://dx.doi.org/10.1155/2014/947560
---------- CHICAGO ----------
Petrera, E., Níttolo, A.G., Alché, L.E. "Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro" . BioMed Research International 2014 (2014).
http://dx.doi.org/10.1155/2014/947560
---------- MLA ----------
Petrera, E., Níttolo, A.G., Alché, L.E. "Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro" . BioMed Research International, vol. 2014, 2014.
http://dx.doi.org/10.1155/2014/947560
---------- VANCOUVER ----------
Petrera, E., Níttolo, A.G., Alché, L.E. Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells in Vitro. BioMed Res. Int. 2014;2014.
http://dx.doi.org/10.1155/2014/947560