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Oxidative stress plays critical roles in the pathogenesis of diabetes, hypertension, and atherosclerosis; some authors reported that fat accumulation correlates to systemic oxidative stress in human and mice, but cellular redox environment effect on lipid accumulation is still unclear. In our laboratory we used mouse embryonic fibroblasts (undifferentiated cells: CC), which are capable of differentiating into mature adipocytes (differentiated cells: DC) and accumulate lipids, as obesity model. Here we analyzed the role of the well-known antioxidant and glutathione precursor N-acetylcysteine (NAC) in cellular MAPK modulation and lipid accumulation. We evaluated the effect of NAC on the adipogenic differentiation pathway using different doses: 0.01, 0.1, 1 and 5 mM; no toxic doses in these cells. A dose of 5 mM NAC [DCN-5] provoked a significant decrease in triglyceride accumulation (72±10 [DCN-5] vs 169±15 [DC], p<0.01), as well in Oil Red O stained neutral lipid content (120±2 [DCN-5] vs 139±12 [DC], p<0.01). Molecular mechanisms responsible for adipogenic differentiation involve increase of the expression of phosphoERK1/2 and phosphoJNK, 5 mM NAC treatment inhibited both pERK1/2 and pJNK protein levels. We also evaluated the mitotic clonal expansion (MCE) which takes place during adipogenesis and observed an increase in DC at a rate of 1.5 cells number compared to CC at day 2, whereas the highest doses of NAC significantly inhibited MCE. Our results suggest that NAC inhibits lipid accumulation and the MAPK phosphorylation in mouse embryonic fibroblasts during adipogenic differentiation and further contribute to probe the importance of cellular redox environment in adipogenesis. © 2016 The Authors.


Documento: Artículo
Título:N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes
Autor:Pieralisi, A.; Martini, C.; Soto, D.; Vila, M.C.; Calvo, J.C.; Guerra, L.N.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires - IQUIBICEN, CONICET, Intendente Güiraldes 2160, Pabellón 2, Buenos Aires, 1428, Argentina
IBYME, CONICET, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:Adipogenesis; Antioxidants; Kinases; Lipids; MEF; N-acetylcysteine; acetylcysteine; Janus kinase; mitogen activated protein kinase; phosphoERK; phosphoJNK; phosphoprotein; triacylglycerol; unclassified drug; acetylcysteine; phosphotransferase; adipocyte; adipogenesis; animal cell; animal tissue; antioxidant activity; Article; cell count; cell differentiation; cell function; cell level; cell proliferation; cell stress; cell viability; concentration response; controlled study; disease model; drug cytotoxicity; drug effect; embryo; enzyme phosphorylation; lipid storage; mitotic clonal expansion; mouse; nonhuman; obesity; priority journal; procedures concerning cells; protein expression; protein lipid interaction; signal transduction; 3T3-L1 cell line; adipocyte; animal; cytology; drug effects; fibroblast; lipid metabolism; mammalian embryo; metabolism; phosphorylation; 3T3-L1 Cells; Acetylcysteine; Adipocytes; Adipogenesis; Animals; Cell Differentiation; Embryo, Mammalian; Fibroblasts; Lipid Metabolism; Mice; Phosphorylation; Phosphotransferases
Página de inicio:39
Página de fin:44
Título revista:Redox Biology
Título revista abreviado:Redox Biol.
CAS:acetylcysteine, 616-91-1; Janus kinase, 161384-16-3; mitogen activated protein kinase, 142243-02-5; phosphotransferase, 9031-09-8, 9031-44-1; Acetylcysteine; Phosphotransferases


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---------- APA ----------
Pieralisi, A., Martini, C., Soto, D., Vila, M.C., Calvo, J.C. & Guerra, L.N. (2016) . N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes. Redox Biology, 9, 39-44.
---------- CHICAGO ----------
Pieralisi, A., Martini, C., Soto, D., Vila, M.C., Calvo, J.C., Guerra, L.N. "N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes" . Redox Biology 9 (2016) : 39-44.
---------- MLA ----------
Pieralisi, A., Martini, C., Soto, D., Vila, M.C., Calvo, J.C., Guerra, L.N. "N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes" . Redox Biology, vol. 9, 2016, pp. 39-44.
---------- VANCOUVER ----------
Pieralisi, A., Martini, C., Soto, D., Vila, M.C., Calvo, J.C., Guerra, L.N. N-acetylcysteine inhibits lipid accumulation in mouse embryonic adipocytes. Redox Biol. 2016;9:39-44.