Artículo

Preger-Ben Noon, E.; Sabarís, G.; Ortiz, D.M.; Sager, J.; Liebowitz, A.; Stern, D.L.; Frankel, N. "Comprehensive Analysis of a cis-Regulatory Region Reveals Pleiotropy in Enhancer Function" (2018) Cell Reports. 22(11):3021-3031
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Abstract:

Developmental genes can have complex cis-regulatory regions with multiple enhancers. Early work revealed remarkable modularity of enhancers, whereby distinct DNA regions drive gene expression in defined spatiotemporal domains. Nevertheless, a few reports have shown that enhancers function in multiple developmental stages, implying that enhancers can be pleiotropic. Here, we have studied the activity of the enhancers of the shavenbaby gene throughout D. melanogaster development. We found that all seven shavenbaby enhancers drive expression in multiple tissues and developmental stages. We explored how enhancer pleiotropy is encoded in two of these enhancers. In one enhancer, the same transcription factor binding sites contribute to embryonic and pupal expression, revealing site pleiotropy, whereas for a second enhancer, these roles are encoded by distinct sites. Enhancer pleiotropy may be a common feature of cis-regulatory regions of developmental genes, and site pleiotropy may constrain enhancer evolution in some cases. Preger-Ben Noon et al. find that shavenbaby gene enhancers contain regulatory information for driving several expression patterns (i.e., enhancers are pleiotropic) and that, in some cases, the transcription factor binding sites that activate these enhancers are reused during development. © 2018 The Author(s)

Registro:

Documento: Artículo
Título:Comprehensive Analysis of a cis-Regulatory Region Reveals Pleiotropy in Enhancer Function
Autor:Preger-Ben Noon, E.; Sabarís, G.; Ortiz, D.M.; Sager, J.; Liebowitz, A.; Stern, D.L.; Frankel, N.
Filiación:Howard Hughes Medical Institute (HHMI), Janelia Research Campus, Ashburn, VA 20147, United States
Departamento de Ecología, Genética y Evolución, IEGEBA-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, 1428, Argentina
Department of Molecular Biology, Princeton University, PrincetonNJ 08544, United States
Palabras clave:Drosophila; enhancer; gene regulation; pleitropy; shavenbaby; transcription factor; adult; animal experiment; animal tissue; Article; binding site; controlled study; developmental gene; developmental stage; Drosophila melanogaster; embryo; enhancer region; female; gene; gene activity; gene expression; gene function; genetic code; genetic regulation; insect development; male; nonhuman; pleiotropy; priority journal; svb gene
Año:2018
Volumen:22
Número:11
Página de inicio:3021
Página de fin:3031
DOI: http://dx.doi.org/10.1016/j.celrep.2018.02.073
Título revista:Cell Reports
Título revista abreviado:Cell Rep.
ISSN:22111247
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_22111247_v22_n11_p3021_PregerBenNoon

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Citas:

---------- APA ----------
Preger-Ben Noon, E., Sabarís, G., Ortiz, D.M., Sager, J., Liebowitz, A., Stern, D.L. & Frankel, N. (2018) . Comprehensive Analysis of a cis-Regulatory Region Reveals Pleiotropy in Enhancer Function. Cell Reports, 22(11), 3021-3031.
http://dx.doi.org/10.1016/j.celrep.2018.02.073
---------- CHICAGO ----------
Preger-Ben Noon, E., Sabarís, G., Ortiz, D.M., Sager, J., Liebowitz, A., Stern, D.L., et al. "Comprehensive Analysis of a cis-Regulatory Region Reveals Pleiotropy in Enhancer Function" . Cell Reports 22, no. 11 (2018) : 3021-3031.
http://dx.doi.org/10.1016/j.celrep.2018.02.073
---------- MLA ----------
Preger-Ben Noon, E., Sabarís, G., Ortiz, D.M., Sager, J., Liebowitz, A., Stern, D.L., et al. "Comprehensive Analysis of a cis-Regulatory Region Reveals Pleiotropy in Enhancer Function" . Cell Reports, vol. 22, no. 11, 2018, pp. 3021-3031.
http://dx.doi.org/10.1016/j.celrep.2018.02.073
---------- VANCOUVER ----------
Preger-Ben Noon, E., Sabarís, G., Ortiz, D.M., Sager, J., Liebowitz, A., Stern, D.L., et al. Comprehensive Analysis of a cis-Regulatory Region Reveals Pleiotropy in Enhancer Function. Cell Rep. 2018;22(11):3021-3031.
http://dx.doi.org/10.1016/j.celrep.2018.02.073