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Wurm, C.A.; Kolmakov, K.; Göttfert, F.; Ta, H.; Bossi, M.; Schill, H.; Berning, S.; Jakobs, S.; Donnert, G.; Belov, V.N.; Hell, S.W. "Novel red fluorophores with superior performance in STED microscopy" (2012) Optical Nanoscopy. 1(1):1-7
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Abstract:

In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.

Registro:

Documento: Artículo
Título:Novel red fluorophores with superior performance in STED microscopy
Autor:Wurm, C.A.; Kolmakov, K.; Göttfert, F.; Ta, H.; Bossi, M.; Schill, H.; Berning, S.; Jakobs, S.; Donnert, G.; Belov, V.N.; Hell, S.W.
Filiación:Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, Göttingen, 37077, Germany
Department of Neurology, Georg-August University of Göttingen Medical School, Göttingen, 37073, Germany
Inquimae Conicet-UBA, Pabellon 2, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:Bioconjugation; Confocal; Fluorescence; Microscopy; Nanoscopy; Rhodamines; STED; Stimulated emission depletion; Super-resolution; Bio-conjugation; Confocal; Nanoscopy; Rhodamines; STED; Stimulated emission depletion; Super resolution; Fluorescence; Fluorescence microscopy; Fluorophores; Microscopic examination; Optical microscopy; Phosphorylation; Quantum yield; Stimulated emission
Año:2012
Volumen:1
Número:1
Página de inicio:1
Página de fin:7
DOI: http://dx.doi.org/10.1186/2192-2853-1-7
Título revista:Optical Nanoscopy
Título revista abreviado:Opt. Nanoscopy
ISSN:21922853
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21922853_v1_n1_p1_Wurm

Referencias:

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Citas:

---------- APA ----------
Wurm, C.A., Kolmakov, K., Göttfert, F., Ta, H., Bossi, M., Schill, H., Berning, S.,..., Hell, S.W. (2012) . Novel red fluorophores with superior performance in STED microscopy. Optical Nanoscopy, 1(1), 1-7.
http://dx.doi.org/10.1186/2192-2853-1-7
---------- CHICAGO ----------
Wurm, C.A., Kolmakov, K., Göttfert, F., Ta, H., Bossi, M., Schill, H., et al. "Novel red fluorophores with superior performance in STED microscopy" . Optical Nanoscopy 1, no. 1 (2012) : 1-7.
http://dx.doi.org/10.1186/2192-2853-1-7
---------- MLA ----------
Wurm, C.A., Kolmakov, K., Göttfert, F., Ta, H., Bossi, M., Schill, H., et al. "Novel red fluorophores with superior performance in STED microscopy" . Optical Nanoscopy, vol. 1, no. 1, 2012, pp. 1-7.
http://dx.doi.org/10.1186/2192-2853-1-7
---------- VANCOUVER ----------
Wurm, C.A., Kolmakov, K., Göttfert, F., Ta, H., Bossi, M., Schill, H., et al. Novel red fluorophores with superior performance in STED microscopy. Opt. Nanoscopy. 2012;1(1):1-7.
http://dx.doi.org/10.1186/2192-2853-1-7