Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

DEET and IR3535 are insect repellents present worldwide in commercial products; their efficacy has been mainly evaluated in mosquitoes. This study compares the toxicological effects and the behavioral responses induced by both repellents on the blood-sucking bug Triatoma infestans Klug (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. When applied topically, the Median Lethal Dose (72 h) for DEET was 220.8 μg/insect. Using IR3535, topical application of 500 μg/insect killed no nymphs. The minimum concentration that produced repellency was the same for both compounds: 1,15 μg/cm2. The effect of a mixture DEET:IR3535 1:1 was similar to that of their pure components. Flushing out was assessed in a chamber with a shelter containing groups of ten nymphs. The repellents were aerosolized on the shelter and the number of insects leaving it was recorded for 60 min. During that time, 0.006 g/m3 of the positive control tetramethrin flushed out 76.7% of the nymphs, while 1.76 g/m3 of DEET or IR3535 flushed out 30 and 0%, respectively. The concentrations required for both compounds to produce toxicity or flushing out are too high to have any practical applications. However, they showed a promising repellency. Additional research should be done to evaluate their possible use for personal protection against T. infestans bites. © 2017 Reynoso et al.

Registro:

Documento: Artículo
Título:Toxicity, repellency and flushing out in Triatoma infestans (Hemiptera: Reduviidae) exposed to the repellents DEET and IR3535
Autor:Reynoso, M.M.N.; Seccacini, E.A.; Calcagno, J.A.; Zerba, E.N.; Alzogaray, R.A.
Filiación:UNIDEF, CITEDEF, CONICET, CIPEIN, Villa Martelli, Buenos Aires, Argentina
Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y de Diagnostico (CEBBAD), Departamento de Ciencias Naturales y Antropológicas, CONICET, Ciudad Autónoma de Buenos Aires, Argentina
Instituto de Investigación e Ingeniería Ambiental (3IA), Universidad Nacional de San Martín (UNSAM), San Martín, Buenos Aires, Argentina
Palabras clave:Blood-sucking bugs; Diethyltoluamide; Ethyl 3-[acetyl(butyl)amino]propanoate; Locomotor activity; ethyl 3 [acetyl(butyl)amino]propanoate; insect repellent; n,n diethyl 3 methylbenzamide; tetramethrin; unclassified drug; aerosol; Article; bioassay; flushing out bioassay; intoxication; lethal dose; molting; nonhuman; repellency bioassay; Triatoma infestans
Año:2017
Volumen:2017
Número:5
DOI: http://dx.doi.org/10.7717/peerj.3292
Título revista:PeerJ
Título revista abreviado:PeerJ
ISSN:21678359
CAS:tetramethrin, 7696-12-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21678359_v2017_n5_p_Reynoso

Referencias:

  • Abramson, C.I., Aldana, E., Sulbaran, E., Exposure to citral, cinnamon and ruda disrupts the life cycle of a vector of Chagas Disease (2007) American Journal of Environmental Sciences, 3, pp. 7-8
  • (2012) Registro de repelentes de insectos inscriptos (actualizado al 18/06/2012), , http://www.anmat.gov.ar/listados/Listado_repelentes_jun_2012pdf, ANMAT, Buenos Aires. Available at
  • Aggarwal, K.K., Tripathi, A.K., Prajapati, V., Kumar, S., Toxicity of 1,8-cineole towards three species of stored product coleopterans (2001) International Journal of Tropical Insect Science, 21, pp. 155-160
  • Alzogaray, R.A., Behavioral and toxicological responses of Rhodnius prolixus (Hemiptera: Reduviidae) to the insect repellents DEET and IR3535 (2016) Journal of Medical Entomology, 53, pp. 387-393
  • Alzogaray, R.A., Fontán, A., Zerba, E.N., Evaluation of hyperactivity produced by pyrethroid treatment on nymph III of Triatoma infestans (Hemiptera: Reduviidae) (1997) Archives of Insect Biochemistry and Physiology, 35, p. 323333
  • Alzogaray, R.A., Fontán, A., Zerba, E.N., Repellency of deet to nymphs of Triatoma in- festans (2000) Medical and Veterinary Entomology, 14, pp. 6-10
  • Antwi, F.B., Shama, L.M., Peterson, R.K., Risk assessments for the insect repellents DEET and picaridin (2008) Regulatory Toxicology and Pharmacology, 51, pp. 31-36
  • Avelar-Freitas, B.A., Dias, J.V.L., Tibães, G.G., Rocha, C.A., Bahia-Oliveira, G.H., Grael, C.F.F., Pires, H.H.R., The effect of Ageratum fastigiatum extract on Rhodnius nasutus, vector of Chagas disease (2012) Brazilian Journal of Pharmacognosy, 23, pp. 366-369
  • Barnard, D.R., Bernier, U.R., Posey, K.H., Xue, D., Repellency of IR3535, KBR3023, para-menthane-3,8-diol, and Deet to black salt marsh mosquitoes (Diptera: Culicidae) in the Everglades National Park (2002) Journal of Medical Entomology, 39, pp. 895-899
  • Barnard, D.R., Xue, R.D., Laboratory evaluation of mosquito repellents against Aedes albopictus, Culex nigripalpus, and Ochlerotatus triseriatus (Diptera: Culicidae) (2004) Journal of Medical Entomology, 41, pp. 726-730
  • Cilek, J.E., Petersen, J.L., Hallmon, C.F., Comparative efficacy of IR3535 and DEET as repellents against adult Aedes aegypti and Culex quinquefasciatus (2004) Journal of the American Mosquito Control Association, 20, pp. 299-304
  • Coelho, A.A.M., De Paula, J.E., Spíndola, L.S., Insecticidal activity on cerrado plants extracts on Rhodnius milesi Carcavallo, Rocha, Garvão and Jurberg (Hemiptera: Reduviidae), under laboratory conditions (2006) Neotropical Entomology, 35, pp. 133-138
  • Corbel, V., Stankiewicz, M., Pennetier, C., Fournier, D., Stojan, J., Girard, E., Dimitrov, M., Lapied, B., Evidence for inhibition of cholinesterases in insect and mammalian nervous system by the insect repellent DEET (2009) BMC Biology, 7, pp. 47-57
  • De Oliveira Filho, A.M., Differences of susceptibility of five triatomine species to pyrethroid insecticidesimplications for Chagas disease vector control (1999) Memorias do Instituto Oswaldo Cruz, 94, pp. 425-428
  • Debboun, M., Strickman, D., Insect repellents and associated personal protection for a reduction in human disease (2013) Medical and Veterinary Entomology, 27, pp. 1-9
  • (2000) N, N-Diethyl-meta-toluamide (DEET). Risk characterization document, , Sacramento: EPA
  • Ferrero, A., González, J., Chopa, C., Biological activity of Schinus molle on Triatoma infestans (2006) Fitoterapia, 77, pp. 381-383
  • Fradin, M.S., Day, J.F., Comparative efficacy of insect repellents against mosquito bites (2002) New England Journal of Medicine, 347, pp. 13-18
  • Gammon, D.W., Neural effects of allethrin on the free walking cockroach Periplaneta americana: and investigation using defined doses at 15 and 32 °C (1978) Journal of Pesticide Science, 9, pp. 79-81
  • Gomes, S.P., Favero, S., Assessment of the insecticidal potential of Eucalyptus urograndis essential oil against Rhodnius neglectus Lent (Hemiptera: Reduviidae) (2013) Neotropical Entomology, 42, pp. 431-435
  • Gürtler, R.E., Schweigmann, N.J., Cécere, M.C., Chuit, R., Wisnivesky-Colli, C., Comparison of two sampling methods for domestic populations of Triatoma infestans in north-west Argentina (1993) Medical and Veterinary Entomology, 7, pp. 238-242
  • Gürtler, R.E., Vázquez Prokopec, G.M., Ceballos, L.A., Lund Petersen, C., Salomón, O.D., Comparison between two artificial shelter units and timed manual collections for detecting peridomestic Triatoma infestans (Hemiptera: Reduviidae) in rural northwestern Argentina (2001) Journal of Medical Entomology, 38, pp. 429-436
  • Lent, H., Wygodzinsky, P., Revision of the triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease (1979) Bulletin of the American Museum of Natural History, 163, pp. 125-520
  • (2002) POLO PC: a user's guide for probit or logit analysis, , Berkeley: LeOra Software
  • López, B., López, M., Aragón, L., Tereschuk, M., Slaris, A., Feresin, G., Zygadlo, J., Tapia, A.A., Composition and anti-insect activity of essential oils from Tagetes L (2011) species (Asteraceae, Helenieae) on Ceratitis capitata Wiederman and Tri- atoma infestans Klug. Journal of Agricultural and Food Chemistry, 59, pp. 5286-5292
  • Mello, C., Uzeda, C., Bernardino, M., Mendonça-Lopes, D., Kelecom, A., Fevereiro, P., Guerra, M., González, M., Effects of the essential oil obtained from Pilocarpus spicatus Saint-Hilarie (Rutaceae) on the development of Rhodnius prolixus nymphae (2007) Brazilian Journal of Pharmacognosy, 17, pp. 514-520
  • Minoli, S., Palottini, F., Crespo, J.G., Manrique, G., Dislodgement effect of natural semiochemicals released by disturbed triatomines: a possible alternative monitoring tool (2013) Journal of Vector Ecology, 38, pp. 353-360
  • Moretti, A., Zerba, E.N., Alzogaray, R.A., Behavioral and toxicological responses of Rhodnius prolixus and Triatoma infestans (Hemiptera: Reduviidae) to ten monoterpene alcohols (2013) Journal of Medical Entomology, 50, pp. 1046-1054
  • Moretti, A., Zerba, E.N., Alzogaray, R.A., Lethal and sublethal effects of eucalyptol on Triatoma infestans and Rhodnius prolixus, vectors of Chagas disease (2015) Entomologia Experimentalis et Applicata, 154, pp. 62-70
  • Nentwig, G., Use of repellents as prophylactic agents (2003) Parasitology Research, 1, pp. S40-S48
  • N'Guessan, R., Rowland, M., Traore Lamizana, M., Kesse, N.B., Carnevale, P., Evaluation of synthetic repellents on mosquito nets in experimental huts against insecticide-resistant Anopheles gambiae and Culex quinquefasciatus mosquitoes (2006) Tropical Medicine & International Health, 100, pp. 1091-1097
  • Osimitz, T.G., Murphy, J.V., Fell, L.A., Page, B., Adverse events associated with the use of insect repellents containing N, N-diethyl-m-toluamide (DEET) (2010) Regulatory Toxicology and Pharmacology, 56, pp. 93-99
  • Picollo, M.I., Vassena, C., Orihuela, P., Barrios, S., Zaidemberg, M., Zerba, E.N., High resistance to pyrethroid insecticides associated to the ineffectiveness of field treatments in Triatoma infestans (Hemiptera, Reduvidae) from the north of Argentina (2005) Journal of Medical Entomology, 42, pp. 637-642
  • Pinchin, R., De Oliveira Filho, A.M., Pereira, A.C.B., The flushing-out activity of pyrethrum and synthetic pyrethroids on Panstrongilus megistus, a vector of Chagas' disease (1980) Transactions of the Royal Society of Tropical Medicine and Hygiene, 75, pp. 691-694
  • Pohlit, A.M., Ribeiro Rezende, A., Lopes Baldin, E.L., Peporine Lopes, N., Ferreira de Andrade Neto, V., Plant extracts, isolated phytochemicals, and plant- derived agents which are lethal to arthropod vectors of human tropical diseasesa review (2011) Planta Medica, 77, pp. 618-630
  • Pridgeon, J.W., Bernier, U.R., Becnel, J.J., Toxicity comparison of eight repellents against four species of female mosquitoes (2009) Journal of the American Mosquito Control Association, 25, pp. 168-173
  • Puccetti, G., (2007) IR3535 (ethyl butylacetylaminoproprionate), pp. 353-360. , In: Debboun M, Frances SP, Strickman D, eds. Insect repellent. Boca Raton: CRC Press
  • Roca-Acevedo, G., Picollo, M.I., Santo-Orihuela, P., Expression of insecticide resistance in immature life stages of Triatoma infestans (Hemiptera: Reduviidae) (2013) Journal of Medical Entomology, 50, pp. 816-818
  • Scheffler, I., Dombrowski, M., (1992) Behavioural responses of German cockroaches (Blattella germanica L.) induced by plant repellents, pp. 107-116. , In: Otto D, Weber B, eds. Insecticides: mechanism of action and resistance. Andover: Intercept
  • Sfara, V., Zerba, E., Alzogaray, R.A., Toxicity of pyrethroids and repellency of diethyltoluamide in two deltamethrin resistant colonies of Triatoma infestans Klug (Hemiptera: Reduviidae) (2006) Memorias Do Instituto Oswaldo Cruz, 101, pp. 89-94
  • Sfara, V., Zerba, E.N., Alzogaray, R.A., Fumigant insecticidal activity and repellent effect of five essential oils and seven monoterpenes in first instar nymphs of Rhodnius prolixus (2009) Journal of Medical Entomology, 46, pp. 511-515
  • Stevens, L., Dorn, P.L., Schmidt, J.O., Klotz, J.H., Lucero, D., Klotz, S.A., Kissing bugs: vectors of Chagas (2011) Advances in Parasitology, 75, pp. 169-192
  • Swale, D.R., Sun, B., Tong, F., Bloomquist, J.R., Neurotoxicity and mode of action of N, N-diethyl-meta-toluamide (DEET) (2014) PLOS ONE, 9
  • Terriquez, J.A., Klotz, S.A., Meister, E.A., Klotz, J.H., Schmidt, J.O., Repellency of DEET, picaridin, and three essential oils to Triatoma rubida (Hemiptera: Reduviidae: Triatominae (2013) Journal of Medical Entomology, 50, pp. 664-667
  • White, G.B., Moore, S.J., (2015) Terminology of insect repellents, pp. 3-30. , In: Debboun M, Frances SP, Strickman D, eds. Insect repellent, handbook. 2nd edition. Boca Raton: CRC Press
  • (2001) IR3535. Interim specification WHO/IS/TC/667/2001, , Geneva: WHO
  • Review of IR3535; KBR 3023; (RS)- methoprene 20% EC, pyriproxyfen 0.5% GR; and lambda-cyhalothrin 2.5% CS (2001) Report of the fourth WHOPES working group meeting, , WHO, Geneva, Switzerland
  • Chagas disease in Latin America: an epidemiological update based on 2010 estimates (2015) Weekly Epidemiological Record, 90, pp. 33-44
  • Xue, R., Muller, G.C., Day, J.F., Commercially available insect repellents and criteria for their use (2015), pp. 3-30. , In: Debboun M, Frances SP, Strickman D, eds. Insect repellent, hand- book. 2nd edition. Boca Raton: CRC Press

Citas:

---------- APA ----------
Reynoso, M.M.N., Seccacini, E.A., Calcagno, J.A., Zerba, E.N. & Alzogaray, R.A. (2017) . Toxicity, repellency and flushing out in Triatoma infestans (Hemiptera: Reduviidae) exposed to the repellents DEET and IR3535. PeerJ, 2017(5).
http://dx.doi.org/10.7717/peerj.3292
---------- CHICAGO ----------
Reynoso, M.M.N., Seccacini, E.A., Calcagno, J.A., Zerba, E.N., Alzogaray, R.A. "Toxicity, repellency and flushing out in Triatoma infestans (Hemiptera: Reduviidae) exposed to the repellents DEET and IR3535" . PeerJ 2017, no. 5 (2017).
http://dx.doi.org/10.7717/peerj.3292
---------- MLA ----------
Reynoso, M.M.N., Seccacini, E.A., Calcagno, J.A., Zerba, E.N., Alzogaray, R.A. "Toxicity, repellency and flushing out in Triatoma infestans (Hemiptera: Reduviidae) exposed to the repellents DEET and IR3535" . PeerJ, vol. 2017, no. 5, 2017.
http://dx.doi.org/10.7717/peerj.3292
---------- VANCOUVER ----------
Reynoso, M.M.N., Seccacini, E.A., Calcagno, J.A., Zerba, E.N., Alzogaray, R.A. Toxicity, repellency and flushing out in Triatoma infestans (Hemiptera: Reduviidae) exposed to the repellents DEET and IR3535. PeerJ. 2017;2017(5).
http://dx.doi.org/10.7717/peerj.3292