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Abstract:

The funnel-web spider Macrothele calpeiana is a charismatic Mygalomorph with a great interest in basic, applied and translational research. Nevertheless, current scarcity of genomic and transcriptomic data of this species clearly limits the research in this non-model organism. To overcome this limitation, we launched the first tissue-specific enriched RNA-seq analysis in this species using a subtractive hybridization approach, with two main objectives, to characterize the specific transcriptome of the putative chemosensory appendages (palps and first pair of legs), and to provide a new set of DNA markers for further phylogenetic studies. We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes. Among specific candidates, we have identified some members of the iGluR and NPC2 families. Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs. Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance. © 2015 Frías-López et al.

Registro:

Documento: Artículo
Título:Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae)
Autor:Frías-López, C.; Almeida, F.C.; Guirao-Rico, S.; Vizueta, J.; Sánchez-Gracia, A.; Arnedo, M.A.; Rozas, J.
Filiación:Departament de Genètica, Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain
Departament de Biologia Animal and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain
Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Intendente Güiraldes y Costanera Norte, s/n, Pabellón II-Ciudad Universitaria, Capital Federal, Argentina
Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain
Palabras clave:Chemosensory system; De novo transcriptome assembly; Molecular markers; Mygalomorphae Phylogeny; RNA-seq; complementary DNA; tissue specific transcriptome; transcriptome; unclassified drug; adult; Article; biodiversity; bootstrapping; chemosensory related gene; chemosensory tissue; data processing; DNA marker; female; gene expression; gene ontology; gene sequence; genomics; housekeeping gene; hybridization; Macrothele calpeiana; molecular biology; molecular genetics; nonhuman; phylogeny; RNA extraction; RNA sequence; spider
Año:2015
Volumen:2015
Número:6
DOI: http://dx.doi.org/10.7717/peerj.1064
Título revista:PeerJ
Título revista abreviado:PeerJ
ISSN:21678359
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21678359_v2015_n6_p_FriasLopez

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Citas:

---------- APA ----------
Frías-López, C., Almeida, F.C., Guirao-Rico, S., Vizueta, J., Sánchez-Gracia, A., Arnedo, M.A. & Rozas, J. (2015) . Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae). PeerJ, 2015(6).
http://dx.doi.org/10.7717/peerj.1064
---------- CHICAGO ----------
Frías-López, C., Almeida, F.C., Guirao-Rico, S., Vizueta, J., Sánchez-Gracia, A., Arnedo, M.A., et al. "Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae)" . PeerJ 2015, no. 6 (2015).
http://dx.doi.org/10.7717/peerj.1064
---------- MLA ----------
Frías-López, C., Almeida, F.C., Guirao-Rico, S., Vizueta, J., Sánchez-Gracia, A., Arnedo, M.A., et al. "Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae)" . PeerJ, vol. 2015, no. 6, 2015.
http://dx.doi.org/10.7717/peerj.1064
---------- VANCOUVER ----------
Frías-López, C., Almeida, F.C., Guirao-Rico, S., Vizueta, J., Sánchez-Gracia, A., Arnedo, M.A., et al. Comparative analysis of tissue-specific transcriptomes in the funnel-web spider Macrothele calpeiana (Araneae, Hexathelidae). PeerJ. 2015;2015(6).
http://dx.doi.org/10.7717/peerj.1064