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Abstract:

Primordial germ cells (PGCs) are the undifferentiated progenitors of gametes. Germline competent PGCs can be developed as a cell-based system for genetic modification in chickens, which provides a valuable tool for transgenic technology with both research and industrial applications. This implies manipulation of PGCs, which, in recent years, encouraged a lot of research focused on the study of PGCs and the way of improving their culture. The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that besides mediating toxic responses to environmental contaminants plays pivotal physiological roles in various biological processes. Since a novel compound that acts as an antagonist of this receptor has been reported to promote expansion of hematopoietic stem cells, we conducted the present study with the aim of determining whether addition of an established AHR antagonist to the standard culture medium used nowadays for in vitro chicken PGCs culture improves ex vivo expansion. We have found that addition of α-naphthoflavone in culture medium promotes the amplification of undifferentiated cells and that this effect is exerted by the blockade of AHR action. Our results constitute the first report of the successful use of a readily available AHR antagonist to improve avian PGCs expansion, and they further extend the knowledge of the effects of AHR modulation in undifferentiated cells. Copyright © 2016 Mary Ann Liebert, Inc.

Registro:

Documento: Artículo
Título:Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity
Autor:Pérez Sáez, J.M.; Bussmann, L.E.; Barañao, J.L.; Bussmann, U.A.
Filiación:Instituto de Biología y Medicina Experimental, CONICET, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:alkaline phosphatase; alpha naphthoflavone; aromatic hydrocarbon receptor; stage specific embryo antigen 1; alpha-naphthoflavone; aromatic hydrocarbon receptor; benzoflavone derivative; animal experiment; Article; chicken; controlled study; culture medium; embryo; flow cytometry; hematopoietic stem cell; immunofluorescence; in vitro study; nonhuman; primordial germ cell; priority journal; stable transfection; animal; antagonists and inhibitors; cell culture; chicken; cytology; drug effects; female; germ cell; signal transduction; Animals; Benzoflavones; Cells, Cultured; Chickens; Female; Germ Cells; Receptors, Aryl Hydrocarbon; Signal Transduction
Año:2016
Volumen:18
Número:3
Página de inicio:154
Página de fin:161
DOI: http://dx.doi.org/10.1089/cell.2016.0015
Título revista:Cellular Reprogramming
Título revista abreviado:Cell. Reprogramming
ISSN:21524971
CAS:alkaline phosphatase, 9001-78-9; alpha naphthoflavone, 604-59-1; alpha-naphthoflavone; Benzoflavones; Receptors, Aryl Hydrocarbon
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_21524971_v18_n3_p154_PerezSaez

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Citas:

---------- APA ----------
Pérez Sáez, J.M., Bussmann, L.E., Barañao, J.L. & Bussmann, U.A. (2016) . Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity. Cellular Reprogramming, 18(3), 154-161.
http://dx.doi.org/10.1089/cell.2016.0015
---------- CHICAGO ----------
Pérez Sáez, J.M., Bussmann, L.E., Barañao, J.L., Bussmann, U.A. "Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity" . Cellular Reprogramming 18, no. 3 (2016) : 154-161.
http://dx.doi.org/10.1089/cell.2016.0015
---------- MLA ----------
Pérez Sáez, J.M., Bussmann, L.E., Barañao, J.L., Bussmann, U.A. "Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity" . Cellular Reprogramming, vol. 18, no. 3, 2016, pp. 154-161.
http://dx.doi.org/10.1089/cell.2016.0015
---------- VANCOUVER ----------
Pérez Sáez, J.M., Bussmann, L.E., Barañao, J.L., Bussmann, U.A. Improvement of Chicken Primordial Germ Cell Maintenance In Vitro by Blockade of the Aryl Hydrocarbon Receptor Endogenous Activity. Cell. Reprogramming. 2016;18(3):154-161.
http://dx.doi.org/10.1089/cell.2016.0015