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Abstract:

Polyamines are essential for Trypanosoma cruzi, the causative agent of Chagas disease. As T. cruzi behaves as a natural auxotrophic organism, it relies on host polyamines biosynthesis. In this paper we obtained a double-transfected T. cruzi parasite that expresses the green fluorescent protein (GFP) and a heterologous ornithine decarboxylase (ODC), used itself as a novel selectable marker. These autotrophic and fluorescent parasites were characterized; the ODC presented an apparent Km for ornithine of 0.51 0.16 mM and an estimated Vvalue of 476.2 nmoles/h/mg of protein. These expressing ODC parasites showed higher metacyclogenesis capacity than the auxotrophic counterpart, supporting the idea that polyamines are engaged in this process. This double-transfected T. cruzi parasite results in a powerful tool - easy to follow by its fluorescence - to study the role of polyamines in Chagas disease pathology and in related processes such as parasite survival, invasion, proliferation, metacyclogenesis, and tissue spreading. © 2011 Jeremías José Barclay et al.

Registro:

Documento: Artículo
Título:Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology
Autor:Barclay, J.J.; Morosi, L.G.; Vanrell, M.C.; Trejo, E.C.; Romano, P.S.; Carrillo, C.
Filiación:Fundación Instituto Leloir-(FIL-IIBBA-) CONICET, Departamento de Química Biológica, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Laboratorio de Biología Celular y Molecular, Instituto de Histología y Embriología-(IHEM-) CONICET, Universidad Nacional de Cuyo, 5500 Mendoza, Argentina
Palabras clave:green fluorescent protein; ornithine; ornithine decarboxylase; polyamine; animal cell; article; auxotrophy; Chagas disease; controlled study; enzyme activity; enzyme kinetics; fluorescence analysis; genetic transfection; nonhuman; parasite survival; parasite transmission; pathogenesis; priority journal; protein expression; Trypanosoma cruzi; Vero cell; Trypanosoma cruzi
Año:2011
Volumen:2011
Número:1
DOI: http://dx.doi.org/10.4061/2011/657460
Título revista:Enzyme Research
Título revista abreviado:Enzyme Res.
ISSN:20900406
CAS:ornithine, 70-26-8, 7006-33-9; ornithine decarboxylase, 9024-60-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20900406_v2011_n1_p_Barclay

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Citas:

---------- APA ----------
Barclay, J.J., Morosi, L.G., Vanrell, M.C., Trejo, E.C., Romano, P.S. & Carrillo, C. (2011) . Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology. Enzyme Research, 2011(1).
http://dx.doi.org/10.4061/2011/657460
---------- CHICAGO ----------
Barclay, J.J., Morosi, L.G., Vanrell, M.C., Trejo, E.C., Romano, P.S., Carrillo, C. "Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology" . Enzyme Research 2011, no. 1 (2011).
http://dx.doi.org/10.4061/2011/657460
---------- MLA ----------
Barclay, J.J., Morosi, L.G., Vanrell, M.C., Trejo, E.C., Romano, P.S., Carrillo, C. "Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology" . Enzyme Research, vol. 2011, no. 1, 2011.
http://dx.doi.org/10.4061/2011/657460
---------- VANCOUVER ----------
Barclay, J.J., Morosi, L.G., Vanrell, M.C., Trejo, E.C., Romano, P.S., Carrillo, C. Trypanosoma cruzi coexpressing ornithine decarboxylase and green fluorescence proteins as a tool to study the role of polyamines in chagas disease pathology. Enzyme Res. 2011;2011(1).
http://dx.doi.org/10.4061/2011/657460