Conferencia

Erra-Balsells, R.; Gholipour, Y.; Nonami, H.; Gelman L.; Hunter A.; Korsunsky A.M.; Ao S.I.; Hukins D.WL. "In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS" (2012) 2012 World Congress on Engineering, WCE 2012. 2197:572-577
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Abstract:

The joint application of a cell pressure probe (PP) and a UV-matrix-assisted laser desorption/ionization time of flight (UV-MALDI-TOF) mass spectrometer was used for picoliter sampling and shotgun metabolite profiling of in situ living single cells of plants. Plant cell wall and plasma membrane properties and water relations of in situ living single cells can be analyzed before sampling the cell sap. With different organic compounds and nanoparticles as UV-MALDI matrix, metabolites from neutral carbohydrates to amino acids and secondary other metabolites can be detected through UV-MALDI-TOF mass spectrometry analyses of picoliter single-cell samples. © 2012 Newswood Limited. All rights reserved.

Registro:

Documento: Conferencia
Título:In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS
Autor:Erra-Balsells, R.; Gholipour, Y.; Nonami, H.; Gelman L.; Hunter A.; Korsunsky A.M.; Ao S.I.; Hukins D.WL.
Filiación:Organic Chemistry Department, CIHIDECAR, University of Buenos Aires, Buenos Aires, 1428-, Argentina
Department of Biomechanical Systems, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, 780-8566, Japan
Palabras clave:Metabolomics; Nanoparticles; Plant cell; Shotgun metabolite profiling; UV-MALDI-TOF; Biomolecules; Cell membranes; Desorption; Mass spectrometry; Metabolites; Nanoparticles; Plant cell culture; Plants (botany); Potential flow; Probes; MALDI-TOF; MALDI-TOF mass spectrometry; Matrix-assisted laser desorption/ionization time of flights; Membrane properties; Metabolite analysis; Metabolite profiling; Metabolomics; Plant cells; Cytology
Año:2012
Volumen:2197
Página de inicio:572
Página de fin:577
Título revista:2012 World Congress on Engineering, WCE 2012
Título revista abreviado:Lect. Notes Eng. Comput. Sci.
ISSN:20780958
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20780958_v2197_n_p572_ErraBalsells

Referencias:

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  • Avatoš, A., Single-cell metabolomics comes of age: New developments in mass spectrometry profiling and imaging (2011) Anal. Chem., 83, pp. 5037-5044
  • Amantonico, A., Oh, J.Y., Sobek, J., Heinemann, M., Zenobi, R., Mass spectrometric method for analyzing metabolites in yeast with single cell sensitivity (2008) Angew. Chem. Int. Ed., 47, pp. 5382-5385
  • Ebert, B., Zöller, D., Erban, A., Fehrle, I., Hartmann, J., Niehl, A., Kopka, J., Fisahn, J., Metabolic profiling of arabidopsis thaliana epidermal cells (2010) J. Exp. Bot., 61, pp. 1321-1335
  • Koek, M.M., Bakels, F., Engel, W., Van Den Maagdenberg, A., Ferrari, M.D., Coulier, L., Hankemeier, T., Metabolic profiling of ultrasmall sample volumes with GC/MS: From microliter to nanoliter samples (2010) Anal. Chem., 82, pp. 156-162
  • Nonami, H., Boyer, J.S., Wall extensibility and cell hydraulic conductivity decrease in enlarging stem tissues at low water potentials (1990) Plant Physiol, 93, pp. 1610-1619
  • Malone, M., Tomos, A.D., A simple pressure-probe method for the determination of volume in higher-plant cells (1990) Planta, 182, pp. 199-203
  • Nonami, H., Schulze, E.-D., Cell water potential, osmotic potential, and turgor in the epidermis and mesophyll of transpiring leaves: Combined measurements with the cell pressure probe and nanoliter osmometer (1989) Planta, 177, pp. 35-46
  • Nonami, H., Boyer, J.S., Direct demonstration of a growth-induced water potential gradient (1993) Plant Physiol, 102, pp. 13-19
  • Gholipour, Y., Nonami, H., Erra-Balsells, R., Application of pressure probe and UV-MALDI-TOF MS for direct analysis of plant underivatized carbohydrates in subpicoliter single-cell cytoplasm extract (2008) J. Am. Soc. Mass Spectrom., 19, pp. 1841-1848
  • Nonami, H., Fukui, S., Erra-Balsells, R., -Carboline alkaloids as matrices for matrix-assisted ultraviolet laser desorption time-of-flight mass spectrometry of proteins and sulfated oligosaccharides: A comparative study using phenylcarbonyl compounds, carbazoles and classical matrices (1997) J. Mass Spectrom., 32, pp. 287-296
  • Gholipour, Y., Nonami, H., Erra-Balsells, R., In situ analysis of plant tissue underivatized carbohydrates and on-probe enzymatic degraded starch by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by using carbon nanotubes as matrix (2008) Anal. Biochem., 383, pp. 159-167
  • Gholipour, Y., Giudicessi, S.L., Nonami, H., Erra-Balsells, R., Diamond, titanium dioxide, titanium silicon oxide, and barium strontium titanium oxide nanoparticles as matrixes for direct matrix-assisted laser desorption/ionization mass spectrometry analysis of carbohydrates in plant tissues (2010) Anal. Chem., 82, pp. 5518-5526
  • Yu, Z., Chen, L.C., Suzuki, H., Ariyada, O., Erra-Balsells, R., Nonami, H., Hiraoka, K., Direct profiling of phytochemicals in tulip tissues and in vivo monitoring of the change of carbohydrate content in tulip bulbs by probe electrospray ionization mass spectrometry (2009) J. Amer. Soc. Mass Spectrom., 20, pp. 2304-2311

Citas:

---------- APA ----------
Erra-Balsells, R., Gholipour, Y., Nonami, H., Gelman L., Hunter A., Korsunsky A.M., Ao S.I.,..., Hukins D.WL. (2012) . In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS. 2012 World Congress on Engineering, WCE 2012, 2197, 572-577.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20780958_v2197_n_p572_ErraBalsells [ ]
---------- CHICAGO ----------
Erra-Balsells, R., Gholipour, Y., Nonami, H., Gelman L., Hunter A., Korsunsky A.M., et al. "In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS" . 2012 World Congress on Engineering, WCE 2012 2197 (2012) : 572-577.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20780958_v2197_n_p572_ErraBalsells [ ]
---------- MLA ----------
Erra-Balsells, R., Gholipour, Y., Nonami, H., Gelman L., Hunter A., Korsunsky A.M., et al. "In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS" . 2012 World Congress on Engineering, WCE 2012, vol. 2197, 2012, pp. 572-577.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20780958_v2197_n_p572_ErraBalsells [ ]
---------- VANCOUVER ----------
Erra-Balsells, R., Gholipour, Y., Nonami, H., Gelman L., Hunter A., Korsunsky A.M., et al. In situ pressure probe sampling of single cell solution from living plants for metabolite analyses with UV-MALDI MS. Lect. Notes Eng. Comput. Sci. 2012;2197:572-577.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20780958_v2197_n_p572_ErraBalsells [ ]