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Abstract:

Isolated 7S and 11S globulins obtained from defeated soy flour were complexated with folic acid (FA) in order to generate nano-carriers for this important vitamin in human nutrition. Fluorescence spectroscopy and dynamic light scattering were applied to follow the nano-complexes formation and for their characterization. Fluorescence experimental data were modeled by the Stern-Volmer and a modified double logarithm approach. The results obtained confirmed static quenching. The number of binding sites on the protein molecule was ~1. The values obtained for the binding constants suggest a high affinity between proteins and FA. Particle size distribution allowed to study the protein aggregation phenomenon induced by FA bound to the native proteins. Z-average manifested a clear trend to protein aggregation. 11S-FA nano-complexes resulted in more polydispersity. ζ-potential of FA nano-complexes did not show a remarkable change after FA complexation. The biological activity of nano-complexes loaded with FA was explored in terms of their capacity to enhance the biomass formation of Lactobacillus casei BL23. The results concerning to nano-complexes inclusion in culture media showed higher bacterial growth. Such a result was attributed to the entry of the acid by the specific receptors concomitantly by the peptide receptors. These findings have technological impact for the use of globulins-FA based nano-complexes in nutraceutical, pharmaceutical and food industries. © 2018 by the authors.

Registro:

Documento: Artículo
Título:Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation
Autor:Ochnio, M.E.; Martínez, J.H.; Allievi, M.C.; Palavecino, M.; Martínez, K.D.; Pérez, O.E.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Intendente Güiraldes, s/n, Ciudad Universitaria, Buenos Aires, CP 1428, Argentina
Consejo Nacional de Investigacion Cientifica y Tecnicas de la Republica Argentina IQUIBICEN-CONICET, Universidad de Buenos Aires, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Intendente Güiraldes, s/n, Ciudad Universitaria, Buenos Aires, CP 1428, Argentina
Consejo Nacional de Investigacion Científica y Técnicas de la Republica Argentina, CONICET, Universidad de Buenos Aires, Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Intendente Güiraldes, s/n, Ciudad Universitaria, Buenos Aires, CP 1428, Argentina
Palabras clave:7S and 11S globulins; Folic acid; Lactobacillus casei BL23; Nano-complexation; Binding sites; Bins; Bioactivity; Fluorescence; Fluorescence spectroscopy; Light scattering; Organic acids; Particle size; Particle size analysis; 11S globulin; Bioactive compounds; Double logarithms; Folic acids; Lactobacillus casei; Peptide receptor; Protein aggregation; Protein molecules; Proteins
Año:2018
Volumen:10
Número:2
DOI: http://dx.doi.org/10.3390/polym10020149
Título revista:Polymers
Título revista abreviado:Polym.
ISSN:20734360
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20734360_v10_n2_p_Ochnio

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Citas:

---------- APA ----------
Ochnio, M.E., Martínez, J.H., Allievi, M.C., Palavecino, M., Martínez, K.D. & Pérez, O.E. (2018) . Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation. Polymers, 10(2).
http://dx.doi.org/10.3390/polym10020149
---------- CHICAGO ----------
Ochnio, M.E., Martínez, J.H., Allievi, M.C., Palavecino, M., Martínez, K.D., Pérez, O.E. "Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation" . Polymers 10, no. 2 (2018).
http://dx.doi.org/10.3390/polym10020149
---------- MLA ----------
Ochnio, M.E., Martínez, J.H., Allievi, M.C., Palavecino, M., Martínez, K.D., Pérez, O.E. "Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation" . Polymers, vol. 10, no. 2, 2018.
http://dx.doi.org/10.3390/polym10020149
---------- VANCOUVER ----------
Ochnio, M.E., Martínez, J.H., Allievi, M.C., Palavecino, M., Martínez, K.D., Pérez, O.E. Proteins as nano-carriers for bioactive compounds. The case of 7S and 11S soy globulins and folic acid complexation. Polym. 2018;10(2).
http://dx.doi.org/10.3390/polym10020149