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Abstract:

The pedunculopontine nucleus (PPN) is part of the Reticular Activating System, and active during waking and REM sleep. Previous results showed that all PPN cells plateau at gamma frequencies and intrinsic membrane oscillations in PPN neurons are mediated by high-threshold N- and P/Q-type Ca2+ channels. The present study was designed to determine whether some PPN cells have only N-, only P/Q-, or both N- and P/Q-type Ca2+ channels. We used patch-clamp recordings in PPN cells in slices from anesthetized rat pups in the presence of synaptic receptor blockers (SB) and Tetrodotoxin (TTX), and applied ramps to induce intrinsic membrane oscillations. We found that all PPN cell types showed gamma oscillations in the presence of SB+TTX when using current ramps. In 50% of cells, the N-type Ca2+ channel blocker ω-Conotoxin-GVIA (ω-CgTx) reduced gamma oscillation amplitude, while subsequent addition of the P/Q-type blocker ω-Agatoxin-IVA (ω-Aga) blocked the remaining oscillations. Another 20% manifested gamma oscillations that were not significantly affected by the addition of ω-CgTx, however, ω-Aga blocked the remaining oscillations. In 30% of cells, ω-Aga had no effect on gamma oscillations, while ω-CgTx blocked them. These novel results confirm the segregation of populations of PPN cells as a function of the calcium channels expressed, that is, the presence of cells in the PPN that manifest gamma band oscillations through only N-type, only P/Q-type, and both N-type and P/Q-type Ca2+ channels. © 2015 The Authors.

Registro:

Documento: Artículo
Título:High-threshold Ca2+ channels behind gamma band activity in the pedunculopontine nucleus (PPN)
Autor:Luster, B.; D’Onofrio, S.; Urbano, F.; Garcia-Rill, E.
Filiación:Center for Translational Neuroscience, University of Arkansas for Medical Sciences, Little Rock, AR, United States
IFIBYNE-CONICET, University of Buenos Aires, Buenos Aires, Argentina
Palabras clave:Arousal; Gamma oscillations; N-type; P/Q-type; Rats; Waking; cadmium; calcium channel; calcium channel blocking agent; calcium channel N type; calcium channel P type; calcium channel Q type; cesium; omega agatoxin IVA; omega conotoxin GVIA; synapse receptor; tetrodotoxin; adult; animal experiment; animal model; animal tissue; Article; calcium current; controlled study; gamma rhythm; hyperpolarization; immunocytochemistry; nonhuman; oscillation; patch clamp technique; pedunculopontine tegmental nucleus; peristaltic pump; rat; REM sleep; signal processing
Año:2015
Volumen:3
Número:6
DOI: http://dx.doi.org/10.14814/phy2.12431
Título revista:Physiological Reports
Título revista abreviado:Physiol. Rep.
ISSN:2051817X
CAS:cadmium, 22537-48-0, 7440-43-9; cesium, 7440-46-2; omega conotoxin GVIA, 107407-86-3; tetrodotoxin, 4368-28-9, 4664-41-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_2051817X_v3_n6_p_Luster

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Citas:

---------- APA ----------
Luster, B., D’Onofrio, S., Urbano, F. & Garcia-Rill, E. (2015) . High-threshold Ca2+ channels behind gamma band activity in the pedunculopontine nucleus (PPN). Physiological Reports, 3(6).
http://dx.doi.org/10.14814/phy2.12431
---------- CHICAGO ----------
Luster, B., D’Onofrio, S., Urbano, F., Garcia-Rill, E. "High-threshold Ca2+ channels behind gamma band activity in the pedunculopontine nucleus (PPN)" . Physiological Reports 3, no. 6 (2015).
http://dx.doi.org/10.14814/phy2.12431
---------- MLA ----------
Luster, B., D’Onofrio, S., Urbano, F., Garcia-Rill, E. "High-threshold Ca2+ channels behind gamma band activity in the pedunculopontine nucleus (PPN)" . Physiological Reports, vol. 3, no. 6, 2015.
http://dx.doi.org/10.14814/phy2.12431
---------- VANCOUVER ----------
Luster, B., D’Onofrio, S., Urbano, F., Garcia-Rill, E. High-threshold Ca2+ channels behind gamma band activity in the pedunculopontine nucleus (PPN). Physiol. Rep. 2015;3(6).
http://dx.doi.org/10.14814/phy2.12431