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Lleras Forero, L.; Narayanan, R.; Huitema, L.F.A.; Vanbergen, M.; Apschner, A.; Peterson-Maduro, J.; Logister, I.; Valentin, G.; Morelli, L.G.; Oates, A.C.; Schulte-Merker, S. "Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock" (2018) eLife. 7
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Abstract:

Segmentation of the axial skeleton in amniotes depends on the segmentation clock, which patterns the paraxial mesoderm and the sclerotome. While the segmentation clock clearly operates in teleosts, the role of the sclerotome in establishing the axial skeleton is unclear. We severely disrupt zebrafish paraxial segmentation, yet observe a largely normal segmentation process of the chordacentra. We demonstrate that axial entpd5+ notochord sheath cells are responsible for chordacentrum mineralization, and serve as a marker for axial segmentation. While autonomous within the notochord sheath, entpd5 expression and centrum formation show some plasticity and can respond to myotome pattern. These observations reveal for the first time the dynamics of notochord segmentation in a teleost, and are consistent with an autonomous patterning mechanism that is influenced, but not determined by adjacent paraxial mesoderm. This behavior is not consistent with a clock-type mechanism in the notochord. © Lleras Forero et al.

Registro:

Documento: Artículo
Título:Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock
Autor:Lleras Forero, L.; Narayanan, R.; Huitema, L.F.A.; Vanbergen, M.; Apschner, A.; Peterson-Maduro, J.; Logister, I.; Valentin, G.; Morelli, L.G.; Oates, A.C.; Schulte-Merker, S.
Filiación:Institute for Cardiovascular Organogenesis and Regeneration, Faculty of Medicine, WWU Münster, Münster, Germany
CiM Cluster of Excellence (EXC-1003-CiM), Münster, Germany
Hubrecht Institute-KNAW & UMC Utrecht, Utrecht, Netherlands
The Francis Crick Institute, London, United Kingdom
Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), CONICET–Partner Institute of the Max Planck Society, Buenos Aires, Argentina
Departamento de Fisica, FCEyN, UBA, Ciudad Universitaria, Buenos Aires, Argentina
Department of Systemic Cell Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany
Department of Cell and Developmental Biology, University College London, London, United Kingdom
Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Sidney Kimmel Medical College at Thomas Jefferson University, Department of Dermatology and Cutaneous Biology, The Joan and Joel Rosenbloom Center for Fibrotic Diseases, Philadelphia, United States
Department of Laboratory Medicine, Laboratory of Hematology, Radboud University medical center, Nijmegen, Netherlands
Palabras clave:amniote; Article; axial patterning; coccygeal vertebra; controlled study; DNA binding; embryo; embryo segmentation; gene expression; in situ hybridization; mesoderm; muscle cell; myotome; nonhuman; notochord; ossification; osteoblast; promoter region; sclerotome; stop codon; teleost; vertebra body; zebra fish
Año:2018
Volumen:7
DOI: http://dx.doi.org/10.7554/eLife.33843
Título revista:eLife
Título revista abreviado:eLife
ISSN:2050084X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_2050084X_v7_n_p_LlerasForero

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Citas:

---------- APA ----------
Lleras Forero, L., Narayanan, R., Huitema, L.F.A., Vanbergen, M., Apschner, A., Peterson-Maduro, J., Logister, I.,..., Schulte-Merker, S. (2018) . Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock. eLife, 7.
http://dx.doi.org/10.7554/eLife.33843
---------- CHICAGO ----------
Lleras Forero, L., Narayanan, R., Huitema, L.F.A., Vanbergen, M., Apschner, A., Peterson-Maduro, J., et al. "Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock" . eLife 7 (2018).
http://dx.doi.org/10.7554/eLife.33843
---------- MLA ----------
Lleras Forero, L., Narayanan, R., Huitema, L.F.A., Vanbergen, M., Apschner, A., Peterson-Maduro, J., et al. "Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock" . eLife, vol. 7, 2018.
http://dx.doi.org/10.7554/eLife.33843
---------- VANCOUVER ----------
Lleras Forero, L., Narayanan, R., Huitema, L.F.A., Vanbergen, M., Apschner, A., Peterson-Maduro, J., et al. Segmentation of the zebrafish axial skeleton relies on notochord sheath cells and not on the segmentation clock. eLife. 2018;7.
http://dx.doi.org/10.7554/eLife.33843