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Abstract:

The fast expansion of human population around La Paz, Bolivia (3,200–4,100 m.a.s.l.) triggered new suburban settlements in nearby areas in valleys and mountain feet. The white mesquite, Prosopis alba Griseb. (Leguminosae), is a resource (originally used by native communities) that is strongly affected by changes in land use. A gradient in the level of disturbance is found moving away from the La Paz city toward less altitude areas. The main objective of this study was to characterize genetically three P. alba populations with different levels of human disturbance located at different altitudes in Bolivia, in order to provide some guidelines for management and conservation of these species. Based on 10 SSR loci, the populations showed high level of genetic diversity in comparison with other forest species. The population less disturbed and situated at the lowest altitude was the most variable (He = 0.51–0.42), whereas the less variable was the most disturbed and situated at the highest altitude. Heterozygote excess was observed in all populations. Most of genetic diversity (99%) is contained within populations. Genetic differentiation among populations is low (1%), suggesting low gene flow among populations. No evidence of recent bottlenecks events was detected. The estimates of the effective population size were low in all populations. The results are in agreement with the hypothesis that genetic diversity is reduced by the impact of anthropic disturbance in the population located at higher altitude in comparison with the lightly disturbed situated at lower altitude and farther from urban settlements. © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Registro:

Documento: Artículo
Título:Genetic diversity and differentiation among Prosopis alba (Leguminosae) populations from dry valleys of Bolivia with different levels of human disturbance and altitude
Autor:Bessega, C.; Pometti, C.; López, R.P.; Larrea-Alcázar, D.; Fortunato, R.H.; Saidman, B.; Vilardi, J.C.
Filiación:Departamento Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Ecología, Genética y Evolución (IEGEBA), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina
Facultad de Ciencias Puras y Naturales, Carrera de Biología, Universidad Mayor de San Andrés, La Paz, Bolivia
Asociación Boliviana para la Investigación y Conservación de Ecosistemas Andino-Amazónicos (ACEAA), La Paz, Bolivia
N. Repetto y Los Reseros s.n., Hurlingham (1686), Argentina - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Recursos Biológicos (CIRN–CNIA, INTA), Buenos Aires, Argentina
Palabras clave:altitude; bottleneck; genetic diversity; homozygote excess; SSR
Año:2018
Volumen:8
Número:22
Página de inicio:11309
Página de fin:11321
DOI: http://dx.doi.org/10.1002/ece3.4610
Título revista:Ecology and Evolution
Título revista abreviado:Ecology and Evolution
ISSN:20457758
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20457758_v8_n22_p11309_Bessega

Referencias:

  • Agapow, P.M., Burt, A., Indices of multilocus linkage disequilibrium (2001) Molecular Ecology Notes, 1, pp. 101-102. , https://doi.org/10.1046/j.1471-8278.2000.00014.x
  • Alden, J., Loopstra, C., Genetic diversity and population structure in Picea glauca on an altitudinal gradient in interior Alaska (1987) Canadian. Journal of Forest Research, 17, pp. 1519-1526
  • Beck, S., Paniagua, N., Yevara, M., La vegetación y uso de la tierra del altiplano y los valles en el oeste del departamento de Tarija, Bolivia (2001) Historia, Ambiente y Sociedad en Tarija, Bolivia, pp. 47-94. , S. Beck, P. D. PaniaguaN, (Eds.),, Instituto de Ecología-Universidad Mayor de San Andrés, School of Geography- University of Leeds, La Paz
  • Bessega, C., Pometti, C.L., Miller, J.T., Watts, R., Saidman, B.O., Vilardi, J.C., New microsatellite loci for Prosopis alba and P. chilensis (Fabaceae) (2013) Appl Plant Sci, 1 (5), p. 1200324. , https://doi.org/10.3732/apps.1200324
  • Bessega, C., Ferreyra, L., Julio, N., Montoya, S., Saidman, B.O., Vilardi, J.C., Mating system parameters in species of genus Prosopis (Leguminosae) (2000) Hereditas, 132, pp. 19-27. , https://doi.org/10.1111/j.1601-5223.2000.00019.x
  • Bessega, C., Pometti, C.L., Ewens, M., Saidman, B.O., Vilardi, J.C., Strategies for conservation for disturbed Prosopis alba (Leguminosae, Mimosoidae) forests based on mating system and pollen dispersal parameters (2011) Tree Genetics and Genomes, 8, pp. 277-288. , https://doi.org/10.1007/s11295-011-0439-6
  • Bessega, C., Pometti, C.L., Ewens, M., Saidman, B.O., Vilardi, J.C., Fine-scale spatial genetic structure analysis in two Argentine populations of Prosopis alba (Mimosoideae) with different levels of ecological disturbance (2016) European Journal of Forest Research, 135, pp. 495-505. , https://doi.org/10.1007/s10342-016-0948-9
  • Bolgeri, M.J., (2016) Caracterización de movimientos migratorios en guanacos (Lama guanicoe) y patrones de depredación por pumas (Puma concolor) en la Payunia, Mendoza. UNIVERSIDAD NACIONAL DE COMAHUE CENTRO REGIONAL UNIVERSITARIO BARILOCHE, , https://rdi.uncoma.edu.ar/bitstream/handle/123456789/164/BOLGERI%2C%20tesis%20doctoral.pdf?sequence=1&isAllowed=y, Doctoral Thesis
  • Burkart, A., A monograph of the genus Prosopis (Leguminosae subfam. Mimosoidae) (1976) Journal Arnold Arboretum, 57, pp. 219-249
  • Caballero, A., Bravo, I., Wang, J., Inbreeding load and purging: Implications for the short-term survival and the conservation management of small populations (2016) Heredity, 1-9. , https://doi.org/10.1038/hdy.2016.80
  • Campos, C.M., Campos, V.E., Velez, M.S., Albanese, M.S., Rodríguez, M.D., Giannoni, S.M., (2012) Interacciones entre Prosopis flexuosa y la fauna: Animales dispersores y depredadores de semillas, , https://www.researchgate.net/publication/264672490_Interacciones_entre_Prosopis_flexuosa_y_la_fauna_animales_dispersores_y_depredadores_de_semillas, Conference Paper, Reunión Nacional Del Algarrobo, at Universidad Nacional De Córdoba, Facultad De Ciencias Agropecuarias, Secretaría De Extensión
  • Cascante, A., Quesada, M., Lobo, J.J., Fuchs, E.A., Effects of dry forest fragmentation on the reproductive success and genetic structure of the tree Samanea saman (2002) Conservation Biology, 16, pp. 137-147
  • Cockerham, C.C., Variance and gene frequencies (1969) Evolution, 23, pp. 72-84
  • Collevatti, R.G., Grattapaglia, D., Hay, J.D., Population genetic structure of the endarged tropical tree species Caryocar brasiliense based on variability at microsatellite loci (2001) Molecular Ecology, 10, pp. 349-356
  • (2017) R: A language and environment for statistical computing, , https://www.r-project.org/, Vienna, Austria, R Foundation for Statistical Computing
  • Cornuet, J.M., Luikart, G., Description and power analysis of two test for detecting recent population bottlenecks from allelic frequency data (1996) Genetics, 144, pp. 2001-2014
  • daSilva Carvalho, C.C., Ribeiro, M.C., Cortes, M.C., Galleti, M., Collevatti, R., Contemporary and historic factors influence differently genetic differentiation and diversity in a tropical palm (2015) Heredity, 115, pp. 216-224. , https://doi.org/10.1038/hdy.2015.30
  • DiRienzo, A., Peterson, A.C., Garza, J.C., Valdes, A.M., Slatkin, M., Freimer, N.B., Mutational processes of simple sequence repeat loci in human populations (1994) Proceedings of the National Academy of Sciences of the United States of America, 91, pp. 3166-3170. , https://doi.org/10.1073/pnas.91.8.3166
  • Do, C., Waples, R.S., Peel, D., Macbeth, G.M., Tilliet, B.J., Ovenden, J.R., NeEstimator V2.1 reimplementation of software for the estimation of contemporary effective population size (Ne) from genetic data (2014) Molecular Ecology Resources, 14, pp. 209-214
  • England, P.R., Cornuet, J.M., Berthier, P., Tallman, D.A., Luikart, G., Estimating effective population size from linkage disequilibrium: Severe bias in small samples (2006) Conservation Genetics, 7, pp. 303-308. , https://doi.org/10.1007/s10592-005-9103-8
  • Genisse, J., Palacios, R.A., Hoc, P.S., Carrizo, R., Moffat, L., Mom, M.P., Picca, Observaciones sobre la biologia floral de Prosopis (Leguminosae, Mimosoidae). I1 Fases florales y visitantes en el distrito Chaqueño Serrano (1990) Darwiniana, 30, pp. 71-85
  • Grivet, D., Sork, V.L., Davis, W.R.D., Conserving the evolutionary potential of California valley oak (Quercus lobate Nee): A multivariate genetic approach to conservation planning (2008) Molecular Ecology, 17, pp. 139-156
  • Guo, S.W., Thompson, E.A., Performing the exact test of Hardy-Weinberg proportions for multiple alleles (1992) Biometrics, 48, pp. 361-372
  • Hafdıs Hanna, E., Kuss, P., Stöcklin, J., Isolated populations of a rare alpine plant show high genetic diversity and considerable population differentiation (2009) Annals of Botany, 104, pp. 1313-1322. , https://doi.org/10.1093/aob/mcp242
  • Hamrick, J.L., Effects of life history traits on genetic diversity in plant species (1996) Philosophical Transactions of the Royal Society of London. Series B, 351, pp. 1291-1298. , https://doi.org/10.1098/rstb.1996.0112
  • Hamrick, J.L., Response of forest tree to global environmental changes (2004) Forest Ecology and Management, 197, pp. 323-335
  • Hamrick, J.L., Godt, M.J.W., Sherman-Broyles, S.L., Factors influencing levels of genetic diversity in woody plant species (1992) New Forests, 6, pp. 5-124. , https://doi.org/10.1007/BF00120641
  • Hardy, O.J., Vekemans, X., SPAGEDi: A versatile computer program to analyse spatial genetic structure at the individual or population levels (2002) Molecular Ecology Notes, 2, pp. 618-620. , https://doi.org/10.1046/j.1471-8286.2002.00305.x
  • Hastings, A., Complex interactions between dispersal and dynamics – lessons from coupled logistic equations (1993) Ecology, 74, pp. 1362-1372. , https://doi.org/10.2307/1940066
  • Hedrick, P.W., A standardized genetic differentiation measure (2005) Evolution, 59, pp. 1633-1638. , https://doi.org/10.1111/j.0014-3820.2005.tb01814.x
  • Hill, W.G., Estimation of effective population size from data on linkage disequilibrium (1981) Genetical Research, 38, pp. 209-216. , https://doi.org/10.1017/S0016672300020553
  • Jombart, T., Adegenet: A R package for the multivariate analysis of genetic markers (2008) Bioinformatics, 24, pp. 1403-1405. , https://doi.org/10.1093/bioinformatics/btn129
  • Jombart, T., Ahmed, I., Adegenet 1.3-1: new tools for the analysis of genomewide SNP data (2011) Bioinformatics, , https://doi.org/10.1093/bioinformatics/btr521
  • Jump, A.S., Penuelas, J., Genetic effects of chronic habitat fragmentation in a wind-pollinated tree (2006) Proceedings of the National Academy of Sciences of the United States of America, 103, pp. 8096-8100. , https://doi.org/10.1073/pnas.0510127103
  • Kalinowski, S.T., Taper, M.L., Maximum likelihood estimation of the frequency of null alleles at microsatellite loci (2006) Conservation Genetics, 7, pp. 991-995. , https://doi.org/10.1007/s10592-006-9134-9
  • Kamvar, Z.N., Brooks, J.C., Grunwald, N.J., Novel R tools for analysis of genome-wide population genetic data with emphasis on clonality (2015) Frontiers in Genetics, 6, p. 208. , https://doi.org/10.3389/fgene.2015.00208
  • Kamvar, Z.N., Tabima, J.F., Grunwald, N.J., Poppr: An R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction (2014) PeerJ, 2, p. 281. , https://doi.org/10.7717/peerj.281
  • Keenan, K., McGinnity, P., Cross, T.F., Crozier, W.W., Prodohl, P.A., diveRsity: An R package for the estimation and exploration of population genetics parameters and their associated errors (2013) Methods in Ecology and Evolution, 4, pp. 782-788. , https://doi.org/10.1111/2041-210X.12067
  • Keyhonbadi, N., Roland, J., Matter, S.F., Stobeck, C., Genetic differentiation and gene flow among populations of the alpine butterfly Parnassius smintheus, vary with landscape connectivity (2005) Molecular Ecology, 14, pp. 1897-1909
  • Körner, C., (2003) Alpine plant life, , 2nd ed, Heidelberg, Germany, Springer
  • Kramer, A.T., Ison, J.L., Ashley, M.V., Howe, H.F., The paradox of forest fragmentation genetics (2008) Conservation Biology, 22, pp. 878-885. , https://doi.org/10.1111/j.1523-1739.2008.00944.x
  • Ledig, F.T., Hodgskiss, P.D., Jacob-Cervantes, V., Genetic diversity, mating system, and conservation of a Mexican subalpine relict, Picea mexicana Martinez (2002) Conservation Genetics, 3, pp. 113-122
  • Loiselle, B.A., Sork, V.L., Nason, J., Graham, C., Spatial genetic structure of a tropical understory shrub, Psychotria officinalis (Rubiaceae) (1995) American Journal of Botany, 82, pp. 1420-1425
  • Long, J.C., The allelic correlation structure of Gainj and Kalam-speaking people. I. The estimation and interpretation of Wright’s F-statistics (1986) Genetics, 112, pp. 629-647
  • Lowe, A.J., Boshier, D., Ward, M., Bacles, C.F.E., Navarro, C., Genetic resource impacts of habitat loss and degradation; reconciling empirical evidence and predicts theory for neotropical trees (2005) Heredity, 95, pp. 255-273
  • Luikart, G., Allendorf, F.W., Cornuet, J.M., Sherwin, W.B., Distortion of allele frequency distributions provides a test for recent population bottlenecks (1997) Journal of Heredity, 89 (3), pp. 238-247
  • Luikart, G., Cornuet, J.M., Empirical evaluation of a test for identifying recently bottlenecked populations from allele frequency data (1998) Cons. Biol., 12, pp. 228-237
  • Maldonado, D.E., Loayza, A.P., Garcia, E., Pacheco, L.F., Qualitative aspects of the effectiveness of Culpeo foxes (Lycalopex culpaeus) as dispersers of Prosopis alba (Fabaceae) in a Bolivian dry valley (2018) Acta Oecologica, 87, pp. 29-33. , https://doi.org/10.1016/j.actao.2018.02.005
  • Maldonado, D.E., Pacheco, L.F., Saavedra, L.V., Legitimidad en la dispersión de semillas de algarrobo (Prosopis flexuosa, Fabaceae) por zorro andino (Lycalopex culpaeus, Canidae) en el Valle de La Paz (Bolivia) (2014) Ecología En Bolivia, 49 (2), pp. 93-97
  • Manel, S., Gugerli, F., Thuiller, W., Alvarez, N., Legendre, P., Holderegger, R., Taberlet, P., Broad scale adaptative genetic variation in alpine plants is driven by temperature and precipitation (2012) Molecular Ecology, 21 (15), pp. 3729-3738
  • Mares, M.A., Enders, F.A., Kingsolver, J.M., Neff, J.L., Simpson, B.B., (1977) Mesquite: Its biology in two desert ecosystems, US/IBP synthesis Series, pp. 124-149. , B. B. Simpson, (Ed.),, Stroudsburg, PA, Hutchinson and Ross
  • Mathiasen, P., Rovere, A.E., Premoli, A.C., Genetic Structure and early effects of Inbreeding in fragmented temperate forests of a self-incompatible Tree, Embothirium coccineum (2007) Conservation Biology, 23, pp. 232-240
  • Maya Garcia, R., Arizaga, S., Cuevas Reyes, P., Peñaloza- Ramirez, J.M., Rocha Ramirez, V., Oyama, K., Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation (2017) Plant Diversity, 39, pp. 13-19. , https://doi.org/10.1016/j.pld.2016.09.005
  • McKean, S., Robinson, D., (1996) Catálogo bibliográfico sobre el uso y manejo de la biodiversidad en Bolivia, , La Paz, Bolivia, Ministerio de Desarrollo Sostenible y Medio Ambiente, Secretaría Nacional de Recursos Naturales y Medio ambiente, Sub Secretaria de Recursos Naturales, Dirección Nacional de Conservación de la Diversidad
  • Mosca, E., Eckerrt, A., Di Pierro, E., Rocchini, D., La Porta, N., Belletti, P., Neale, D., The geographical and environmental determinants of genetic diversity for four alpine conifers of European Alps (2012) Molecular Ecology, 21 (22), pp. 5530-5545
  • Mottura, M.C., Finkeldey, R., Verga, A.R., Gailing, O., Development and characterization of microsatellite markers for Prosopis chilensis and Prosopis flexuosa and cross-species amplification (2005) Molecular Ecology Notes, 5, pp. 487-489
  • Nagashiro, N., Los recursos forestales y sus características de uso (1992) Conservación de la Diversidad Biológica en Bolivia, pp. 265-291. , M. Marconi, (Ed.),, La Paz, Bolivia, CDC-Bolivia/USAIDBolivia
  • Nei, M., Estimation of average heterozygosity and genetic distance for small number of individuals (1978) Genetics, 89, pp. 583-590
  • Nei, M., Chesser, R., Estimation of fixation indices and gene diversities (1983) Human Genetics, 47, pp. 253-259. , https://doi.org/10.1111/j.1469-1809.1983.tb00993.x
  • Newton, A.C., Allnut, T.R., Gillies, A.C.M., Lowe, A.J., Ennos, R.A., Molecular phylogeography intraspecific variation and the conservation of tree species (1999) Trends in Ecology of Evolution, 15, pp. 140-145. , https://doi.org/10.1016/S0169-5347(98)01555-9
  • Nybom, H., Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants (2004) Molecular Ecology, 13, pp. 1143-1155. , https://doi.org/10.1111/j.1365-294X.2004.02141.x
  • Palsbøll, P.J., Berube, M., Allendorf, F.W., Identification of management units using population genetic data (2006) TRENDS in Ecology and Evolution, 22, pp. 11-16. , https://doi.org/10.1016/j.tree.2006.09.003
  • Peakall, R., Smouse, P.E., GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research (2006) Molecular Ecology Notes, 6, pp. 288-295. , https://doi.org/10.1111/j.1471-8286.2005.01155.x
  • Peakall, R., Smouse, P.E., GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research – an update (2012) Bioinformatics, 28, pp. 2537-2539. , https://doi.org/10.1093/bioinformatics/bts460
  • Piry, S., Luikart, G., Cornuet, J.M., BOTTLENECK: A computer program for detecting recent reductions in the effective population size using allele frequency data (1999) Journal of Heredity, 90, pp. 502-503
  • Pometti, C.L., Pizzo, B., Brunetti, M., Macchioni, N., Ewens, M., Saidman, B.O., Argentinean native wood species: Physical and mechanical characterization of some Prosopis species and Acacia aroma (Leguminosae; Mimosoideae) (2009) Bioresource Technology, 100 (6), pp. 1999-2004. , https://doi.org/10.1016/j.biortech.2008.09.061
  • Premoli, A., Isosyme polimorphisms provide evidence of clinal variation with elevation in Nothophagus pumilio (2003) Journal of Heredity, 94 (3), pp. 218-226
  • Pritchard, J.K., Wen, X., Falush, D., (2009) STRUCTURE ver. 2.3, , https://pritch.bsd.uchicago.edu/, Chicago, IL, University of Chicago, Retrieved from
  • Pritchard, J.K., Stephens, M., Donnelly, P., Inference of population structure using multilocus genotype data (2000) Genetics, 155, pp. 945-959
  • Quiroga, M.P., Premoli, A.C., Genetic patterns in Podocarpus parlatorei reveal the long-term persistence of cold-tolerant elements in the southern Yungas (2007) Journal of Biogeography, 34, pp. 447-455
  • Reynolds, H.G., Some interrelations of the Merriam kangaroo rat to velvet mesquite (1954) Journal of Range Management, 7, pp. 176-180
  • Roig, F.A., Aportes a la Etnobotánica del Género Prosopis (1993) Contribuciones Mendocinas a la quinta Reuni6n Regional para América Latina y el Caribe de la Red de Forestación del CIID, pp. 99-121. , Unidades de Botánica y Fisiología vegetal IADIZA
  • Rousset, F., Raymond, M., Testing heterozygote excess and deficiency (1995) Genetics, 140, pp. 1413-1419
  • Shen, D., Bo, W., Xu, F., Wu, R., Genetic diversity and population structure of the Tibetan poplar (Populus szechuanica var. tibetica) along an altitude gradient (2014) BMC Genetics, 15 (1), p. S11
  • Sork, V.L., Smouse, P.E., Genetic analysis of landscape connectivity in tree populations (2006) Landscape Ecology, 21, pp. 821-836. , https://doi.org/10.1007/s10980-005-5415-9
  • StatSoft, I., (2012) Electronic statistics textbook, , https://www.statsoft.com/textbook/, Tulsa, OK, StatSoft
  • Taira, H., Tsumura, Y., Tomaru, N., Ohba, K., Regeneration system and genetic diversity of Cryptomeria japonica growing at different altitudes (1997) Canadian Journal of Forest Research, 27, pp. 447-452
  • Vidaurre, P.J., Paniagua, N., Moraes, R.M., Etnobotánica en los Andes de Bolivia (2006) Botánica Económica de los Andes Centrales, pp. 224-238. , M. Moraes, R. B. Øllgaard, L. P. Kvist, F. Borchsenius, H. Balslev, (Eds.),, La Paz, Universidad Mayor de San Andrés
  • Villanueva, A., (1997) Tipos de relación bosquecomunidad y normas tradicionales de acceso al bosque en la zona de Tariquía, p. 124. , p)., La Paz, Bolivia, Los casos de las comunidades Chiquiacá y Motoví, PROBONA
  • Wang, J., A comparison of single sample estimators of effective population sizes from genetic marker data (2016) Molecular Ecology, 25, pp. 4692-4711. , https://doi.org/10.1111/mec.13725
  • Waples, R.S., A bias correction for estimates of effective population size bases on linkage disequilibrium at unlinked gene loci (2006) Conservation Genetics, 7, pp. 167-184
  • Waples, R.S., Do, C., LdNe: A program for estimating effective population size from data on linkage disequilibrium (2008) Molecular Ecology Resources, 8, pp. 753-756
  • Wei, X., Meng, H., Jiang, M., Landscape genetics structure of a streamside tree species Euptelea pleiospermum (Eupteleaceae): Contrasting roles of river valley and mountain ridge (2013) Plos One, 8 (6)
  • Williams, J.H., Arnold, M.I., Sources of genetic structure in the woody perennial Betula occidentalus (2001) International Journal of Plant Sciences, 162, pp. 1097-1109
  • Young, A., Boyle, T., Brown, T., The population genetic consequences of habitat fragmentation for plants (1996) Trends in Ecology & Evolution, 11, pp. 413-418
  • Zhang, X., Korpelainen, H., Li, C., Microsatellite variation of Quercus aquifolioides populations varying altitudes in the Wolong natural reserve of China (2006) Silva Fennica, 40, pp. 407-415

Citas:

---------- APA ----------
Bessega, C., Pometti, C., López, R.P., Larrea-Alcázar, D., Fortunato, R.H., Saidman, B. & Vilardi, J.C. (2018) . Genetic diversity and differentiation among Prosopis alba (Leguminosae) populations from dry valleys of Bolivia with different levels of human disturbance and altitude. Ecology and Evolution, 8(22), 11309-11321.
http://dx.doi.org/10.1002/ece3.4610
---------- CHICAGO ----------
Bessega, C., Pometti, C., López, R.P., Larrea-Alcázar, D., Fortunato, R.H., Saidman, B., et al. "Genetic diversity and differentiation among Prosopis alba (Leguminosae) populations from dry valleys of Bolivia with different levels of human disturbance and altitude" . Ecology and Evolution 8, no. 22 (2018) : 11309-11321.
http://dx.doi.org/10.1002/ece3.4610
---------- MLA ----------
Bessega, C., Pometti, C., López, R.P., Larrea-Alcázar, D., Fortunato, R.H., Saidman, B., et al. "Genetic diversity and differentiation among Prosopis alba (Leguminosae) populations from dry valleys of Bolivia with different levels of human disturbance and altitude" . Ecology and Evolution, vol. 8, no. 22, 2018, pp. 11309-11321.
http://dx.doi.org/10.1002/ece3.4610
---------- VANCOUVER ----------
Bessega, C., Pometti, C., López, R.P., Larrea-Alcázar, D., Fortunato, R.H., Saidman, B., et al. Genetic diversity and differentiation among Prosopis alba (Leguminosae) populations from dry valleys of Bolivia with different levels of human disturbance and altitude. Ecology and Evolution. 2018;8(22):11309-11321.
http://dx.doi.org/10.1002/ece3.4610