Artículo

Sánchez, M.C.; Alvarez Sedó, C.; Chaufan, G.R.; Romanato, M.; Da Cuña, R.; Lo Nostro, F.; Calvo, J.C.; Fontana, V. "In vitro effects of endosulfan-based insecticides on mammalian sperm" (2018) Toxicology Research. 7(1):117-126
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Abstract:

Endosulfan is an organochloride insecticide extensively used in several countries to protect crops from pests. As several studies indicate that endosulfan can affect human and animal development, the aim of this study was to analyse whether sperm parameters and the process of chromatin decondensation could be altered by endosulfan in mice sperm. Spermatozoa from cauda epididymis were obtained from mature male mice and incubated in the presence of two commercial formulations (CFs) of endosulfan (Master® and Zebra Ciagro®) or the active ingredient (AI) alone. A significant decrease in the percentage motility and viability of spermatozoa with respect to controls was found. In vitro decondensation was performed in the presence of glutathione and heparin. Spermatozoa incubated with the AI, endosulfan Master® and endosulfan Zebra Ciagro® showed an increase in chromatin decondensation. In addition, the TUNEL assay showed that DNA fragmentation was significantly higher when sperm were incubated with either one of the CFs when compared to the AI or controls. The ultrastructure analysis of sperm cells showed evident changes in the structure of the plasma and acrosome membranes of sperm incubated with endosulfan AI or the CFs. These results suggest that endosulfan can affect sperm integrity and in vitro chromatin decondensation as well as DNA fragmentation. © 2017 The Royal Society of Chemistry.

Registro:

Documento: Artículo
Título:In vitro effects of endosulfan-based insecticides on mammalian sperm
Autor:Sánchez, M.C.; Alvarez Sedó, C.; Chaufan, G.R.; Romanato, M.; Da Cuña, R.; Lo Nostro, F.; Calvo, J.C.; Fontana, V.
Filiación:Instituto de Biología y Medicina Experimental (IByME-CONICET), Buenos Aires, Argentina
Centro de Estudios en Genética y Reproducción (CEGyR), Armenia
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Enzimología, Estrés y Metabolismo (LEEM), CONICET-Universidad de Buenos Aires, Instituto de Química Biológica, Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Ecotoxicología Acuática, Buenos Aires, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA), Buenos Aires, Argentina
Palabras clave:endosulfan; glutathione; glutathione transferase; heparin; superoxide dismutase; acrosome; animal experiment; Article; chromatin; controlled study; DNA fragmentation; enzyme activity; epididymis; in vitro study; lipid peroxidation; male; mammal; mouse; nonhuman; oxidative stress; priority journal; seminal plasma; spermatozoon; spermatozoon motility; spermatozoon penetration; transmission electron microscopy; TUNEL assay
Año:2018
Volumen:7
Número:1
Página de inicio:117
Página de fin:126
DOI: http://dx.doi.org/10.1039/c7tx00251c
Título revista:Toxicology Research
Título revista abreviado:Toxicol. Res.
ISSN:2045452X
CAS:endosulfan, 115-29-7; glutathione, 70-18-8; glutathione transferase, 50812-37-8; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_2045452X_v7_n1_p117_Sanchez

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Citas:

---------- APA ----------
Sánchez, M.C., Alvarez Sedó, C., Chaufan, G.R., Romanato, M., Da Cuña, R., Lo Nostro, F., Calvo, J.C.,..., Fontana, V. (2018) . In vitro effects of endosulfan-based insecticides on mammalian sperm. Toxicology Research, 7(1), 117-126.
http://dx.doi.org/10.1039/c7tx00251c
---------- CHICAGO ----------
Sánchez, M.C., Alvarez Sedó, C., Chaufan, G.R., Romanato, M., Da Cuña, R., Lo Nostro, F., et al. "In vitro effects of endosulfan-based insecticides on mammalian sperm" . Toxicology Research 7, no. 1 (2018) : 117-126.
http://dx.doi.org/10.1039/c7tx00251c
---------- MLA ----------
Sánchez, M.C., Alvarez Sedó, C., Chaufan, G.R., Romanato, M., Da Cuña, R., Lo Nostro, F., et al. "In vitro effects of endosulfan-based insecticides on mammalian sperm" . Toxicology Research, vol. 7, no. 1, 2018, pp. 117-126.
http://dx.doi.org/10.1039/c7tx00251c
---------- VANCOUVER ----------
Sánchez, M.C., Alvarez Sedó, C., Chaufan, G.R., Romanato, M., Da Cuña, R., Lo Nostro, F., et al. In vitro effects of endosulfan-based insecticides on mammalian sperm. Toxicol. Res. 2018;7(1):117-126.
http://dx.doi.org/10.1039/c7tx00251c