Boccardo, N.A.; Segretin, M.E.; Hernandez, I.; Mirkin, F.G.; Chacón, O.; Lopez, Y.; Borrás-Hidalgo, O.; Bravo-Almonacid, F.F. "Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials" (2019) Scientific Reports. 9(1)
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Plants are continuously challenged by pathogens, affecting most staple crops compromising food security. They have evolved different mechanisms to counterattack pathogen infection, including the accumulation of pathogenesis-related (PR) proteins. These proteins have been implicated in active defense, and their overexpression has led to enhanced resistance in nuclear transgenic plants, although in many cases constitutive expression resulted in lesion-mimic phenotypes. We decided to evaluate plastid transformation as an alternative to overcome limitations observed for nuclear transgenic technologies. The advantages include the possibilities to express polycistronic RNAs, to obtain higher protein expression levels, and the impeded gene flow due to the maternal inheritance of the plastome. We transformed Nicotiana tabacum plastids to co-express the tobacco PR proteins AP24 and β-1,3-glucanase. Transplastomic tobacco lines were characterized and subsequently challenged with Rhizoctonia solani, Peronospora hyoscyami f.sp. tabacina and Phytophthora nicotianae. Results showed that transplastomic plants expressing AP24 and β-1,3-glucanase are resistant to R. solani in greenhouse conditions and, furthermore, they are protected against P.hyoscyami f.sp. tabacina and P. nicotianae in field conditions under high inoculum pressure. Our results suggest that plastid co- expression of PR proteins AP24 and β-1,3-glucanase resulted in enhanced resistance against filamentous pathogens. © 2019, The Author(s).


Documento: Artículo
Título:Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials
Autor:Boccardo, N.A.; Segretin, M.E.; Hernandez, I.; Mirkin, F.G.; Chacón, O.; Lopez, Y.; Borrás-Hidalgo, O.; Bravo-Almonacid, F.F.
Filiación:Laboratorio de Biotecnología Vegetal, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres” (INGEBI-CONICET), Ciudad Autónoma de Buenos Aires, (C1428ADN), Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, (C1428EGA), Argentina
Centro de Ingeniería Genética y Biotecnología (CIGB), La Habana, (10600), Cuba
Shandong Provincial Key Laboratory of Microbial Engineering, School of Biotechnology, Qi Lu University of Technology, Jinan, (250353), China
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, (B1876BXD), Argentina
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.


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---------- APA ----------
Boccardo, N.A., Segretin, M.E., Hernandez, I., Mirkin, F.G., Chacón, O., Lopez, Y., Borrás-Hidalgo, O.,..., Bravo-Almonacid, F.F. (2019) . Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials. Scientific Reports, 9(1).
---------- CHICAGO ----------
Boccardo, N.A., Segretin, M.E., Hernandez, I., Mirkin, F.G., Chacón, O., Lopez, Y., et al. "Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials" . Scientific Reports 9, no. 1 (2019).
---------- MLA ----------
Boccardo, N.A., Segretin, M.E., Hernandez, I., Mirkin, F.G., Chacón, O., Lopez, Y., et al. "Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials" . Scientific Reports, vol. 9, no. 1, 2019.
---------- VANCOUVER ----------
Boccardo, N.A., Segretin, M.E., Hernandez, I., Mirkin, F.G., Chacón, O., Lopez, Y., et al. Expression of pathogenesis-related proteins in transplastomic tobacco plants confers resistance to filamentous pathogens under field trials. Sci. Rep. 2019;9(1).