Artículo

Unsain, N.; Bordenave, M.D.; Martinez, G.F.; Jalil, S.; Von Bilderling, C.; Barabas, F.M.; Masullo, L.A.; Johnstone, A.D.; Barker, P.A.; Bisbal, M.; Stefani, F.D.; Cáceres, A.O. "Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration" (2018) Scientific Reports. 8(1)
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Abstract:

Axonal degeneration occurs in the developing nervous system for the appropriate establishment of mature circuits, and is also a hallmark of diverse neurodegenerative diseases. Despite recent interest in the field, little is known about the changes (and possible role) of the cytoskeleton during axonal degeneration. We studied the actin cytoskeleton in an in vitro model of developmental pruning induced by trophic factor withdrawal (TFW). We found that F-actin decrease and growth cone collapse (GCC) occur early after TFW; however, treatments that prevent axonal fragmentation failed to prevent GCC, suggesting independent pathways. Using super-resolution (STED) microscopy we found that the axonal actin/spectrin membrane-associated periodic skeleton (MPS) abundance and organization drop shortly after deprivation, remaining low until fragmentation. Fragmented axons lack MPS (while maintaining microtubules) and acute pharmacological treatments that stabilize actin filaments prevent MPS loss and protect from axonal fragmentation, suggesting that MPS destruction is required for axon fragmentation to proceed. © 2018 The Author(s).

Registro:

Documento: Artículo
Título:Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration
Autor:Unsain, N.; Bordenave, M.D.; Martinez, G.F.; Jalil, S.; Von Bilderling, C.; Barabas, F.M.; Masullo, L.A.; Johnstone, A.D.; Barker, P.A.; Bisbal, M.; Stefani, F.D.; Cáceres, A.O.
Filiación:Instituto de Investigación Médica Mercedes y Martín Ferreyra, INIMEC, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), UNC, Friuli, Córdoba, 2434 - 5016, Argentina
Universidad Nacional de Córdoba, Córdoba, Argentina
Centro de Investigaciones en Bionanociencias (CIBION), CONICET, Buenos Aires, Argentina
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Biology, University of British Columbia, Kelowna, BC, Canada
Montreal Neurological Institute, McGill University, Montreal, Canada
Instituto Universitario Ciencias Biomédicas de Córdoba (IUCBC), Córdoba, Argentina
Año:2018
Volumen:8
Número:1
DOI: http://dx.doi.org/10.1038/s41598-018-21232-0
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
ISSN:20452322
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_Unsain

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Citas:

---------- APA ----------
Unsain, N., Bordenave, M.D., Martinez, G.F., Jalil, S., Von Bilderling, C., Barabas, F.M., Masullo, L.A.,..., Cáceres, A.O. (2018) . Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration. Scientific Reports, 8(1).
http://dx.doi.org/10.1038/s41598-018-21232-0
---------- CHICAGO ----------
Unsain, N., Bordenave, M.D., Martinez, G.F., Jalil, S., Von Bilderling, C., Barabas, F.M., et al. "Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration" . Scientific Reports 8, no. 1 (2018).
http://dx.doi.org/10.1038/s41598-018-21232-0
---------- MLA ----------
Unsain, N., Bordenave, M.D., Martinez, G.F., Jalil, S., Von Bilderling, C., Barabas, F.M., et al. "Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration" . Scientific Reports, vol. 8, no. 1, 2018.
http://dx.doi.org/10.1038/s41598-018-21232-0
---------- VANCOUVER ----------
Unsain, N., Bordenave, M.D., Martinez, G.F., Jalil, S., Von Bilderling, C., Barabas, F.M., et al. Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration. Sci. Rep. 2018;8(1).
http://dx.doi.org/10.1038/s41598-018-21232-0