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Abstract:

Mechanical stimuli play a key role in many cell functions such as proliferation, differentiation and migration. In the mammary gland, mechanical signals such as the distension of mammary epithelial cells due to udder filling are proposed to be directly involved during lactation and involution. However, the evolution of focal adhesions -specialized multiprotein complexes that mechanically connect cells with the extracellular matrix- during the mammary gland development, as well as the influence of the mechanical stimuli involved, remains unclear. Here we present the use of an equibiaxial stretching device for exerting a sustained normal strain to mammary epithelial cells while quantitatively assessing cell responses by fluorescence imaging techniques. Using this approach, we explored changes in focal adhesion dynamics in HC11 mammary cells in response to a mechanical sustained stress, which resembles the physiological stimuli. We studied the relationship between a global stress and focal adhesion assembly/disassembly, observing an enhanced persistency of focal adhesions under strain as well as an increase in their size. At a molecular level, we evaluated the mechanoresponses of vinculin and zyxin, two focal adhesion proteins postulated as mechanosensors, observing an increment in vinculin molecular tension and a slower zyxin dynamics while increasing the applied normal strain. © 2018 The Author(s).

Registro:

Documento: Artículo
Título:Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress
Autor:Sigaut, L.; Von Bilderling, C.; Bianchi, M.; Burdisso, J.E.; Gastaldi, L.; Pietrasanta, L.I.
Filiación:Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and IFIBA, CONICET-UBA, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
Centro de Microscopías Avanzadas (CMA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, C1428EHA, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, Buenos Aires, C1425FQB, Argentina
Año:2018
Volumen:8
Número:1
DOI: http://dx.doi.org/10.1038/s41598-018-27948-3
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
ISSN:20452322
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_Sigaut

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Citas:

---------- APA ----------
Sigaut, L., Von Bilderling, C., Bianchi, M., Burdisso, J.E., Gastaldi, L. & Pietrasanta, L.I. (2018) . Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress. Scientific Reports, 8(1).
http://dx.doi.org/10.1038/s41598-018-27948-3
---------- CHICAGO ----------
Sigaut, L., Von Bilderling, C., Bianchi, M., Burdisso, J.E., Gastaldi, L., Pietrasanta, L.I. "Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress" . Scientific Reports 8, no. 1 (2018).
http://dx.doi.org/10.1038/s41598-018-27948-3
---------- MLA ----------
Sigaut, L., Von Bilderling, C., Bianchi, M., Burdisso, J.E., Gastaldi, L., Pietrasanta, L.I. "Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress" . Scientific Reports, vol. 8, no. 1, 2018.
http://dx.doi.org/10.1038/s41598-018-27948-3
---------- VANCOUVER ----------
Sigaut, L., Von Bilderling, C., Bianchi, M., Burdisso, J.E., Gastaldi, L., Pietrasanta, L.I. Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress. Sci. Rep. 2018;8(1).
http://dx.doi.org/10.1038/s41598-018-27948-3