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Grasso, E.; Gori, S.; Soczewski, E.; Fernández, L.; Gallino, L.; Vota, D.; Martínez, G.; Irigoyen, M.; Ruhlmann, C.; Lobo, T.F.; Salamone, G.; Mattar, R.; Daher, S.; Leirós, C.P.; Ramhorst, R. "Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells" (2018) Scientific Reports. 8(1)
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Abstract:

During decidualization, endometrial stromal cells undergo reticular stress (RS) and unfolded protein response (UPR), allowing the endoplasmic reticulum-expansion and immunomodulators production. Physiological RS generates the activation of sensing proteins, inflammasome activation and mature-IL-1β secretion, associated with pro-implantatory effects. We focus on the impact of RS and UPR on decidualized cells and whether they induce a physiological sterile inflammatory response through IL-1β production. Human endometrial stromal cell line (HESC) after decidualization treatment with MPA + dibutyryl-cAMP (Dec) increased the expression of RS-sensors (ATF6, PERK and IRE1α) and UPR markers (sXBP1 and CHOP) in comparison with Non-dec cells. Then we found increased NLRP3 expression in Dec cells compared with Non-dec cells. In fact STF-083010 (an IRE1α inhibitor) prevented this increase. Downstream, increased levels of active caspase-1 on Dec cells were detected by FAM-Flica Caspase-1 associated with an increase in IL-1β production. Moreover, the treatment with STF-083010 decreased the invasion index observed in Dec cells, evaluated by an in vitro model of implantation. In endometrial biopsies from recurrent spontaneous abortion patients an increased expression of IRE1α was found in comparison with fertile women; while recurrent implantation failure samples showed a lower expression of sXBP1, TXNIP and NLRP3 than fertile women, suggesting that RS/UPR tenors might condition endometrial receptivity. © 2018, The Author(s).

Registro:

Documento: Artículo
Título:Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells
Autor:Grasso, E.; Gori, S.; Soczewski, E.; Fernández, L.; Gallino, L.; Vota, D.; Martínez, G.; Irigoyen, M.; Ruhlmann, C.; Lobo, T.F.; Salamone, G.; Mattar, R.; Daher, S.; Leirós, C.P.; Ramhorst, R.
Filiación:CONICET, Universidad de Buenos Aires. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales IQUIBICEN, Buenos Aires, Argentina
Institute of Experimental Medicine IMEX-CONICET, National Academy of Sciences, Buenos Aires, Argentina
Instituto de Fertilidad San Isidro, Buenos Aires, Argentina
Department of Obstetrics, Universidade Federal de São Paulo, São Paulo, Brazil
Año:2018
Volumen:8
Número:1
DOI: http://dx.doi.org/10.1038/s41598-018-29779-8
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
ISSN:20452322
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v8_n1_p_Grasso

Referencias:

  • Dekel, N., Gnainsky, Y., Granot, I., Mor, G., Inflammation and implantation (2010) Am. J. Reprod. Immunol., 63, pp. 17-21. , PID: 20059465
  • Weiss, G., Goldsmith, L.T., Taylor, R.N., Bellet, D., Taylor, H.S., Inflammation in reproductive disorders (2009) Reprod. Sci., 16, pp. 216-229. , PID: 19208790
  • Mor, G., Cardenas, I., The immune system in pregnancy: a unique complexity (2010) Am. J. Reprod. Immunol., 63, pp. 425-433. , PID: 20367629
  • Loke, Y.W., King, A., Burrows, T.D., Decidua in human implantation (1995) Hum. Reprod., 10, pp. 14-21. , PID: 8745297
  • Teklenburg, G., Natural selection of human embryos: decidualizing endometrial stromal cells serve as sensors of embryo quality upon implantation (2010) PLoS One, 5. , PID: 20422011
  • Brosens, J.J., Parker, M.G., McIndoe, A., Pijnenborg, R., Brosens, I.A., A role for menstruation in preconditioning the uterus for successful pregnancy (2009) Am. J. Obstet. Gynecol., 200, pp. 615-616
  • Salker, M., Natural selection of human embryos: Impaired decidualization of endometrium disables embryo-maternal interactions and causes recurrent pregnancy loss (2010) PLoS One, 5
  • Grootjans, J., Kaser, A., Kaufman, R.J., Blumberg, R.S., The unfolded protein response in immunity and inflammation (2016) Nat. Publ. Gr., , https://doi.org/10.1038/nri.2016.62
  • Guzel, E., (2017) Endoplasmic Reticulum Stress and Homeostasis in Reproductive Physiology and Pathology, , https://doi.org/10.3390/ijms18040792
  • Gong, J., Molecular signal networks and regulating mechanisms of the unfolded protein response* (2017) J Zhejiang Univ Sci B, 18, pp. 1-14. , PID: 28070992
  • Yang, Y., Pei, X., Jin, Y., Wang, Y., Zhang, C., The roles of endoplasmic reticulum stress response in female mammalian reproduction (2016) Cell Tissue Res., 363, pp. 589-597. , PID: 26022337
  • Bettigole, S.E., Glimcher, L.H., Endoplasmic reticulum stress in immunity (2015) Annu. Rev. Immunol., 33, pp. 107-138. , PID: 25493331
  • Davis, B.K., Ting, J.P.-Y., NLRP3 has a sweet tooth (2010) Nat. Immunol., 11, pp. 105-106. , PID: 20084064
  • Strowig, T., Henao-Mejia, J., Elinav, E., Flavell, R., Inflammasomes in health and disease (2012) Nature, 481, pp. 278-286. , PID: 22258606
  • Lerner, A.G., IRE1α induces thioredoxin-interacting protein to activate the NLRP3 inflammasome and promote programmed cell death under irremediable ER stress (2012) Cell Metab., 16, pp. 250-264. , PID: 22883233
  • Oslowski, C.M., Thioredoxin-interacting protein mediates ER stress-induced β cell death through initiation of the inflammasome (2012) Cell Metab., 16, pp. 265-273. , PID: 22883234
  • Hummasti, S., Hotamisligil, G.S., Endoplasmic reticulum stress and inflammation in obesity and diabetes (2010) Circ. Res., 107, pp. 579-591. , PID: 20814028
  • Grasso, E., VIP induces the decidualization program and conditions the immunoregulation of the implantation process (2017) Mol. Cell. Endocrinol., , https://doi.org/10.1016/j.mce.2017.07.006
  • van Mourik, M.S.M., Macklon, N.S., Heijnen, C.J., Embryonic implantation: cytokines, adhesion molecules, and immune cells in establishing an implantation environment (2009) J. Leukoc. Biol., 85, pp. 4-19. , PID: 18784344
  • Teklenburg, G., Salker, M., Heijnen, C., Macklon, N.S., Brosens, J.J., The molecular basis of recurrent pregnancy loss: impaired natural embryo selection (2010) Mol. Hum. Reprod., 16, pp. 886-895. , PID: 20847090
  • Brosens, J.J., Uterine Selection of Human Embryos at Implantation (2015) Sci. Rep., 4
  • Iwawaki, T., Akai, R., Yamanaka, S., Kohno, K., Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability (2009) Proc. Natl. Acad. Sci. USA, 106, pp. 16657-16662. , PID: 19805353
  • Kawakami, T., Prolonged endoplasmic reticulum stress alters placental morphology and causes low birth weight (2014) Toxicol. Appl. Pharmacol., 275, pp. 134-144. , PID: 24370435
  • Walter, P., Ron, D., The unfolded protein response: from stress pathway to homeostatic regulation (2011) Science, 334, pp. 1081-1086. , PID: 22116877
  • Hu, P., Han, Z., Couvillon, A.D., Kaufman, R.J., Exton, J.H., Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression (2006) Mol. Cell. Biol., 26, pp. 3071-3084. , PID: 16581782
  • Li, J., Wang, J.J., Zhang, S.X., Preconditioning with endoplasmic reticulum stress mitigates retinal endothelial inflammation via activation of X-box binding protein 1 (2011) J. Biol. Chem., 286, pp. 4912-4921. , PID: 21138840
  • Schroder, K., Tschopp, J., The inflammasomes (2010) Cell, 140, pp. 821-832. , PID: 20303873
  • Pontillo, A., Bacterial LPS differently modulates inflammasome gene expression and IL-1β secretion in trophoblast cells, decidual stromal cells, and decidual endothelial cells (2013) Reprod. Sci., 20, pp. 563-566. , PID: 23184659
  • Chiti, F., Dobson, C.M., Protein Misfolding, Amyloid Formation, and Human Disease: A Summary of Progress Over the Last Decade (2017) Annu. Rev. Biochem., 86, pp. 27-68. , PID: 28498720
  • Wang, M., Kaufman, R.J., Protein misfolding in the endoplasmic reticulum as a conduit to human disease (2016) Nature, 529, pp. 326-335. , PID: 26791723
  • Navid, F., Colbert, R.A., Causes and consequences of endoplasmic reticulum stress in rheumatic disease (2017) Nat. Rev. Rheumatol., 13, pp. 25-40. , PID: 27904144
  • Lorenzon-Ojea, A.R., Guzzo, C.R., Kapidzic, M., Fisher, S.J., Bevilacqua, E., Stromal Cell-Derived Factor 2: A Novel Protein that Interferes in Endoplasmic Reticulum Stress Pathway in Human Placental Cells (2016) Biol. Reprod., 95, p. 41. , PID: 27335075
  • Galgani, M., Regulatory T cells, inflammation, and endoplasmic reticulum stress in women with defective endometrial receptivity (2015) Fertil. Steril., 103, pp. 1579-1586. , PID: 25935494
  • Gao, H.-J., Endoplasmic reticulum stress induced by oxidative stress in decidual cells: a possible mechanism of early pregnancy loss (2012) Mol. Biol. Rep., 39, pp. 9179-9186. , PID: 22733488
  • D’Ippolito, S., Inflammosome in the human endometrium: Further step in the evaluation of the ‘maternal side’ (2016) Fertil. Steril, 105, pp. 111-8.e1–4
  • Krikun, G., A novel immortalized human endometrial stromal cell line with normal progestational response (2004) Endocrinology, 145, pp. 2291-2296. , PID: 14726435
  • Straszewski-Chavez, S.L., The isolation and characterization of a novel telomerase immortalized first trimester trophoblast cell line, Swan 71 (2009) Placenta, 30, pp. 939-948. , PID: 19766308

Citas:

---------- APA ----------
Grasso, E., Gori, S., Soczewski, E., Fernández, L., Gallino, L., Vota, D., Martínez, G.,..., Ramhorst, R. (2018) . Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells. Scientific Reports, 8(1).
http://dx.doi.org/10.1038/s41598-018-29779-8
---------- CHICAGO ----------
Grasso, E., Gori, S., Soczewski, E., Fernández, L., Gallino, L., Vota, D., et al. "Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells" . Scientific Reports 8, no. 1 (2018).
http://dx.doi.org/10.1038/s41598-018-29779-8
---------- MLA ----------
Grasso, E., Gori, S., Soczewski, E., Fernández, L., Gallino, L., Vota, D., et al. "Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells" . Scientific Reports, vol. 8, no. 1, 2018.
http://dx.doi.org/10.1038/s41598-018-29779-8
---------- VANCOUVER ----------
Grasso, E., Gori, S., Soczewski, E., Fernández, L., Gallino, L., Vota, D., et al. Impact of the Reticular Stress and Unfolded Protein Response on the inflammatory response in endometrial stromal cells. Sci. Rep. 2018;8(1).
http://dx.doi.org/10.1038/s41598-018-29779-8