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Risnik, D.; Podaza, E.; Almejún, M.B.; Colado, A.; Elías, E.E.; Bezares, R.F.; Fernández-Grecco, H.; Cranco, S.; Sánchez-Ávalos, J.C.; Borge, M.; Gamberale, R.; Giordano, M. "Revisiting the role of interleukin-8 in chronic lymphocytic leukemia" (2017) Scientific Reports. 7(1)
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Abstract:

The proliferation and survival of malignant B cells in chronic lymphocytic leukemia (CLL) depend on signals from the microenvironment in lymphoid tissues. Among a plethora of soluble factors, IL-8 has been considered one of the most relevant to support CLL B cell progression in an autocrine fashion, even though the expression of IL-8 receptors, CXCR1 and CXCR2, on leukemic B cells has not been reported. Here we show that circulating CLL B cells neither express CXCR1 or CXCR2 nor they respond to exogenous IL-8 when cultured in vitro alone or in the presence of monocytes/nurse-like cells. By intracellular staining and ELISA we show that highly purified CLL B cells do not produce IL-8 spontaneously or upon activation through the B cell receptor. By contrast, we found that a minor proportion (<0.5%) of contaminating monocytes in enriched suspensions of leukemic cells might be the actual source of IL-8 due to their strong capacity to release this cytokine. Altogether our results indicate that CLL B cells are not able to secrete or respond to IL-8 and highlight the importance of methodological details in in vitro experiments. © 2017 The Author(s).

Registro:

Documento: Artículo
Título:Revisiting the role of interleukin-8 in chronic lymphocytic leukemia
Autor:Risnik, D.; Podaza, E.; Almejún, M.B.; Colado, A.; Elías, E.E.; Bezares, R.F.; Fernández-Grecco, H.; Cranco, S.; Sánchez-Ávalos, J.C.; Borge, M.; Gamberale, R.; Giordano, M.
Filiación:Laboratorio de Inmunología Oncológica, Instituto de Medicina Experimental (IMEX), CONICET, Academia Nacional de Medicina, Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Hospital General de Agudos Dr. Teodoro Álvarez, Buenos Aires, Argentina
Sanatorio Municipal Dr. Julio Méndez, Buenos Aires, Argentina
Instituto Alexander Fleming, Buenos Aires, Argentina
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Año:2017
Volumen:7
Número:1
DOI: http://dx.doi.org/10.1038/s41598-017-15953-x
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
ISSN:20452322
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v7_n1_p_Risnik

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Citas:

---------- APA ----------
Risnik, D., Podaza, E., Almejún, M.B., Colado, A., Elías, E.E., Bezares, R.F., Fernández-Grecco, H.,..., Giordano, M. (2017) . Revisiting the role of interleukin-8 in chronic lymphocytic leukemia. Scientific Reports, 7(1).
http://dx.doi.org/10.1038/s41598-017-15953-x
---------- CHICAGO ----------
Risnik, D., Podaza, E., Almejún, M.B., Colado, A., Elías, E.E., Bezares, R.F., et al. "Revisiting the role of interleukin-8 in chronic lymphocytic leukemia" . Scientific Reports 7, no. 1 (2017).
http://dx.doi.org/10.1038/s41598-017-15953-x
---------- MLA ----------
Risnik, D., Podaza, E., Almejún, M.B., Colado, A., Elías, E.E., Bezares, R.F., et al. "Revisiting the role of interleukin-8 in chronic lymphocytic leukemia" . Scientific Reports, vol. 7, no. 1, 2017.
http://dx.doi.org/10.1038/s41598-017-15953-x
---------- VANCOUVER ----------
Risnik, D., Podaza, E., Almejún, M.B., Colado, A., Elías, E.E., Bezares, R.F., et al. Revisiting the role of interleukin-8 in chronic lymphocytic leukemia. Sci. Rep. 2017;7(1).
http://dx.doi.org/10.1038/s41598-017-15953-x