Inda, C.; Bonfiglio, J.J.; Dos Santos Claro, P.A.; Senin, S.A.; Armando, N.G.; Deussing, J.M.; Silberstein, S."CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells" (2017) Scientific Reports. 7(1)
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Corticotropin-releasing hormone receptor 1 (CRHR1) activates the atypical soluble adenylyl cyclase (sAC) in addition to transmembrane adenylyl cyclases (tmACs). Both cAMP sources were shown to be required for the phosphorylation of ERK1/2 triggered by activated G protein coupled receptor (GPCR) CRHR1 in neuronal and neuroendocrine contexts. Here, we show that activated CRHR1 promotes growth arrest and neurite elongation in neuronal hippocampal cells (HT22-CRHR1 cells). By characterising CRHR1 signalling mechanisms involved in the neuritogenic effect, we demonstrate that neurite outgrowth in HT22-CRHR1 cells takes place by a sAC-dependent, ERK1/2-independent signalling cascade. Both tmACs and sAC are involved in corticotropin-releasing hormone (CRH)-mediated CREB phosphorylation and c-fos induction, but only sAC-generated cAMP pools are critical for the neuritogenic effect of CRH, further highlighting the engagement of two sources of cAMP downstream of the activation of a GPCR, and reinforcing the notion that restricted cAMP microdomains may regulate independent cellular processes. © 2017 The Author(s).


Documento: Artículo
Título:CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells
Autor:Inda, C.; Bonfiglio, J.J.; Dos Santos Claro, P.A.; Senin, S.A.; Armando, N.G.; Deussing, J.M.; Silberstein, S.
Filiación:Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), CONICET-Partner Institute of the Max Planck Society, Buenos Aires, Argentina
DFBMC, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Max Planck Institute of Psychiatry, Department of Stress Neurobiology and Neurogenetics Molecular Neurogenetics, Munich, Germany
Max Planck Institute for Biology of Ageing, Cologne, Germany
Palabras clave:adenylate cyclase; biological marker; corticotropin releasing factor; corticotropin releasing factor receptor; corticotropin releasing factor receptor 1; cyclic AMP; cyclic AMP responsive element binding protein binding protein; animal; blood; cell culture; cell cycle checkpoint; cell differentiation; cell survival; cytology; human; metabolism; mouse; pyramidal nerve cell; Adenylyl Cyclases; Animals; Biomarkers; Cell Cycle Checkpoints; Cell Differentiation; Cell Survival; Cells, Cultured; Corticotropin-Releasing Hormone; CREB-Binding Protein; Cyclic AMP; Humans; Mice; Pyramidal Cells; Receptors, Corticotropin-Releasing Hormone
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
CAS:adenylate cyclase, 9012-42-4; corticotropin releasing factor, 9015-71-8, 178359-01-8, 79804-71-0, 86297-72-5, 86784-80-7; cyclic AMP, 60-92-4; cyclic AMP responsive element binding protein binding protein, 190209-80-4; Adenylyl Cyclases; Biomarkers; Corticotropin-Releasing Hormone; CREB-Binding Protein; CRF receptor type 1; Cyclic AMP; Receptors, Corticotropin-Releasing Hormone


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---------- APA ----------
Inda, C., Bonfiglio, J.J., Dos Santos Claro, P.A., Senin, S.A., Armando, N.G., Deussing, J.M. & Silberstein, S. (2017) . CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells. Scientific Reports, 7(1).
---------- CHICAGO ----------
Inda, C., Bonfiglio, J.J., Dos Santos Claro, P.A., Senin, S.A., Armando, N.G., Deussing, J.M., et al. "CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells" . Scientific Reports 7, no. 1 (2017).
---------- MLA ----------
Inda, C., Bonfiglio, J.J., Dos Santos Claro, P.A., Senin, S.A., Armando, N.G., Deussing, J.M., et al. "CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells" . Scientific Reports, vol. 7, no. 1, 2017.
---------- VANCOUVER ----------
Inda, C., Bonfiglio, J.J., Dos Santos Claro, P.A., Senin, S.A., Armando, N.G., Deussing, J.M., et al. CAMP-dependent cell differentiation triggered by activated CRHR1 in hippocampal neuronal cells. Sci. Rep. 2017;7(1).