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Abstract:

Successful embryo implantation occurs followed by a local pro-inflammatory response subsequently shifted toward a tolerogenic one. VIP (vasoactive intestinal peptide) has embryotrofic, anti-inflammatory and tolerogenic effects. In this sense, we investigated whether the in vivo treatment with VIP contributes to an immunosuppressant local microenvironment associated with an improved pregnancy outcome in the CBA/JxDBA/2 resorption prone model. Pregnancy induced the expression of VIP, VPAC1 and VPAC2 in the uterus from CBA/JxDBA/2 mating females on day 8.5 of gestation compared with non-pregnant mice. VIP treatment (2nmol/mouse i.p.) on day 6.5 significantly increased the number of viable implantation sites and improved the asymmetric distribution of implanted embryos. This effect was accompanied by a decrease in RORγt and an increase in TGF-β and PPARγ expression at the implantation sites. Moreover, VIP modulated the maternal peritoneal macrophages efferocytosis ability, tested using latex beads-FITC or apoptotic thymocytes, displaying an increased frequency of IL-10-producer F4/80 cells while did not modulate TNF-α and IL-12 secretion. The present data suggest that VIP treatment increases the number of viable embryos associated with an increase in the efferocytic ability of maternal macrophages which is related to an immunosuppressant microenvironment. © 2016, Nature Publishing Group. All rights reserved.

Registro:

Documento: Artículo
Título:VIP treatment prevents embryo resorption by modulating efferocytosis and activation profile of maternal macrophages in the CBAxDBA resorption prone model
Autor:Gallino, L.; Calo, G.; Hauk, V.; Fraccaroli, L.; Grasso, E.; Vermeulen, M.; Leirós, C.P.; Ramhorst, R.
Filiación:Immunopharmacology Laboratory, School of Sciences, University of Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina
Institute of Experimental Medicine IMEX-CONICET, National Academy of Sciences, Buenos Aires, Argentina
Palabras clave:cytokine; retinoid related orphan receptor gamma; vasoactive intestinal polypeptide; vasoactive intestinal polypeptide receptor; animal; biosynthesis; CBA mouse; DBA mouse; drug effects; female; genetics; macrophage; male; metabolism; mouse; nidation; phagocytosis; physiology; tumor microenvironment; Animals; Cellular Microenvironment; Cytokines; Embryo Implantation; Female; Macrophages; Male; Mice; Mice, Inbred CBA; Mice, Inbred DBA; Nuclear Receptor Subfamily 1, Group F, Member 3; Phagocytosis; Receptors, Vasoactive Intestinal Peptide; Vasoactive Intestinal Peptide
Año:2016
Volumen:6
DOI: http://dx.doi.org/10.1038/srep18633
Título revista:Scientific Reports
Título revista abreviado:Sci. Rep.
ISSN:20452322
CAS:vasoactive intestinal polypeptide, 37221-79-7; Cytokines; Nuclear Receptor Subfamily 1, Group F, Member 3; Receptors, Vasoactive Intestinal Peptide; Vasoactive Intestinal Peptide
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20452322_v6_n_p_Gallino

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Citas:

---------- APA ----------
Gallino, L., Calo, G., Hauk, V., Fraccaroli, L., Grasso, E., Vermeulen, M., Leirós, C.P.,..., Ramhorst, R. (2016) . VIP treatment prevents embryo resorption by modulating efferocytosis and activation profile of maternal macrophages in the CBAxDBA resorption prone model. Scientific Reports, 6.
http://dx.doi.org/10.1038/srep18633
---------- CHICAGO ----------
Gallino, L., Calo, G., Hauk, V., Fraccaroli, L., Grasso, E., Vermeulen, M., et al. "VIP treatment prevents embryo resorption by modulating efferocytosis and activation profile of maternal macrophages in the CBAxDBA resorption prone model" . Scientific Reports 6 (2016).
http://dx.doi.org/10.1038/srep18633
---------- MLA ----------
Gallino, L., Calo, G., Hauk, V., Fraccaroli, L., Grasso, E., Vermeulen, M., et al. "VIP treatment prevents embryo resorption by modulating efferocytosis and activation profile of maternal macrophages in the CBAxDBA resorption prone model" . Scientific Reports, vol. 6, 2016.
http://dx.doi.org/10.1038/srep18633
---------- VANCOUVER ----------
Gallino, L., Calo, G., Hauk, V., Fraccaroli, L., Grasso, E., Vermeulen, M., et al. VIP treatment prevents embryo resorption by modulating efferocytosis and activation profile of maternal macrophages in the CBAxDBA resorption prone model. Sci. Rep. 2016;6.
http://dx.doi.org/10.1038/srep18633