Artículo

Paez, A.V.; Pallavicini, C.; Schuster, F.; Valacco, M.P.; Giudice, J.; Ortiz, E.G.; Anselmino, N.; Labanca, E.; Binaghi, M.; Salierno, M.; Martí, M.A.; Cotignola, J.H.; Woloszynska-Read, A.; Bruno, L.; Levi, V.; Navone, N.; Vazquez, E.S.; Gueron, G. "Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell-cell contacts and filopodia-induced zippering in prostate cancer" (2016) Cell death & disease. 7(12):e2570
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Prostate cancer (PCa) cells display abnormal expression of cytoskeletal proteins resulting in an augmented capacity to resist chemotherapy and colonize distant organs. We have previously shown that heme oxygenase 1 (HO-1) is implicated in cell morphology regulation in PCa. Here, through a multi 'omics' approach we define the HO-1 interactome in PCa, identifying HO-1 molecular partners associated with the integrity of the cellular cytoskeleton. The bioinformatics screening for these cytoskeletal-related partners reveal that they are highly misregulated in prostate adenocarcinoma compared with normal prostate tissue. Under HO-1 induction, PCa cells present reduced frequency in migration events, trajectory and cell velocity and, a significant higher proportion of filopodia-like protrusions favoring zippering among neighboring cells. Moreover forced expression of HO-1 was also capable of altering cell protrusions in transwell co-culture systems of PCa cells with MC3T3 cells (pre-osteoblastic cell line). Accordingly, these effects were reversed under siHO. Transcriptomics profiling evidenced significant modulation of key markers related to cell adhesion and cell-cell communication under HO-1 induction. The integration from our omics-based research provides a four molecular pathway foundation (ANXA2/HMGA1/POU3F1; NFRSF13/GSN; TMOD3/RAI14/VWF; and PLAT/PLAU) behind HO-1 regulation of tumor cytoskeletal cell compartments. The complementary proteomics and transcriptomics approaches presented here promise to move us closer to unravel the molecular framework underpinning HO-1 involvement in the modulation of cytoskeleton pathways, pushing toward a less aggressive phenotype in PCa.

Registro:

Documento: Artículo
Título:Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell-cell contacts and filopodia-induced zippering in prostate cancer
Autor:Paez, A.V.; Pallavicini, C.; Schuster, F.; Valacco, M.P.; Giudice, J.; Ortiz, E.G.; Anselmino, N.; Labanca, E.; Binaghi, M.; Salierno, M.; Martí, M.A.; Cotignola, J.H.; Woloszynska-Read, A.; Bruno, L.; Levi, V.; Navone, N.; Vazquez, E.S.; Gueron, G.
Filiación:Department of Biological Chemistry, School of Sciences, FCEN, University of Buenos Aires, IQUIBICEN-CONICET, Buenos Aires, Argentina
Department of Physics, FCEN, University of Buenos Aires, IFIBA-CONICET, Buenos Aires, Argentina
Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Department of Genitourinary Medical Oncology, The University of Texas, MD Anderson Cancer Center, Houston, TX, USA
Pharmacology and Therapeutics Department, Roswell Park Cancer Institute, Buffalo, NY, USA
Palabras clave:conditioned medium; heme oxygenase 1; protein binding; transcriptome; animal; cell communication; cell motion; coculture; conditioned medium; cytoskeleton; DNA microarray; drug effects; enzymology; gene expression regulation; gene regulatory network; genetics; human; male; metabolism; mouse; pathology; pharmacology; prostate tumor; proteomics; pseudopodium; sequence analysis; tandem mass spectrometry; tumor cell line; X ray crystallography; Animals; Cell Communication; Cell Line, Tumor; Cell Movement; Coculture Techniques; Crystallography, X-Ray; Culture Media, Conditioned; Cytoskeleton; Gene Expression Regulation, Neoplastic; Gene Regulatory Networks; Heme Oxygenase-1; Humans; Male; Mice; Oligonucleotide Array Sequence Analysis; Prostatic Neoplasms; Protein Binding; Proteomics; Pseudopodia; Sequence Analysis, RNA; Tandem Mass Spectrometry; Transcriptome
Año:2016
Volumen:7
Número:12
Página de inicio:e2570
DOI: http://dx.doi.org/10.1038/cddis.2016.420
Título revista:Cell death & disease
Título revista abreviado:Cell Death Dis
ISSN:20414889
CAS:Culture Media, Conditioned; Heme Oxygenase-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20414889_v7_n12_pe2570_Paez

Citas:

---------- APA ----------
Paez, A.V., Pallavicini, C., Schuster, F., Valacco, M.P., Giudice, J., Ortiz, E.G., Anselmino, N.,..., Gueron, G. (2016) . Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell-cell contacts and filopodia-induced zippering in prostate cancer. Cell death & disease, 7(12), e2570.
http://dx.doi.org/10.1038/cddis.2016.420
---------- CHICAGO ----------
Paez, A.V., Pallavicini, C., Schuster, F., Valacco, M.P., Giudice, J., Ortiz, E.G., et al. "Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell-cell contacts and filopodia-induced zippering in prostate cancer" . Cell death & disease 7, no. 12 (2016) : e2570.
http://dx.doi.org/10.1038/cddis.2016.420
---------- MLA ----------
Paez, A.V., Pallavicini, C., Schuster, F., Valacco, M.P., Giudice, J., Ortiz, E.G., et al. "Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell-cell contacts and filopodia-induced zippering in prostate cancer" . Cell death & disease, vol. 7, no. 12, 2016, pp. e2570.
http://dx.doi.org/10.1038/cddis.2016.420
---------- VANCOUVER ----------
Paez, A.V., Pallavicini, C., Schuster, F., Valacco, M.P., Giudice, J., Ortiz, E.G., et al. Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell-cell contacts and filopodia-induced zippering in prostate cancer. Cell Death Dis. 2016;7(12):e2570.
http://dx.doi.org/10.1038/cddis.2016.420