Artículo

Muglia, C.; Mercer, N.; Toscano, M.A.; Schattner, M.; Pozner, R.; Cerliani, J.P.; Papa Gobbi, R.; Rabinovich, G.A.; Docena, G.H. "The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine" (2011) Cell Death and Disease. 2(5)
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Abstract:

Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function. © 2011 Macmillan Publishers Limited All rights reserved.

Registro:

Documento: Artículo
Título:The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
Autor:Muglia, C.; Mercer, N.; Toscano, M.A.; Schattner, M.; Pozner, R.; Cerliani, J.P.; Papa Gobbi, R.; Rabinovich, G.A.; Docena, G.H.
Filiación:Laboratorio de Investigaciones Del Sistema Inmune, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Calles 47 y 115, C, 1900 La Plata, Argentina
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Apoptosis; Enterocytes; Galectin-1; Mucosa; Small bowel; caspase; galectin 1; galectin 1; polysaccharide; animal cell; animal experiment; animal model; apoptosis; article; binding site; cell migration; cell proliferation; cell survival; cell viability; controlled study; crypt cell; glycosylation; histopathology; human; human cell; immunoblotting; in vitro study; in vivo study; intestine cell; intestine villus; mitochondrial membrane potential; mitochondrion; mouse; mutant; nonhuman; priority journal; protein analysis; protein expression; protein function; small intestine; small intestine mucosa; wild type; animal; cell death; cell survival; cytology; epithelium cell; genetics; male; metabolism; mouse mutant; Mus; Animals; Cell Death; Cell Proliferation; Cell Survival; Epithelial Cells; Galectin 1; Humans; Intestine, Small; Male; Mice; Mice, Knockout; Polysaccharides
Año:2011
Volumen:2
Número:5
DOI: http://dx.doi.org/10.1038/cddis.2011.44
Título revista:Cell Death and Disease
Título revista abreviado:Cell Death Dis.
ISSN:20414889
CAS:caspase, 186322-81-6; galectin 1, 258495-34-0; Galectin 1; Polysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_20414889_v2_n5_p_Muglia

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Citas:

---------- APA ----------
Muglia, C., Mercer, N., Toscano, M.A., Schattner, M., Pozner, R., Cerliani, J.P., Papa Gobbi, R.,..., Docena, G.H. (2011) . The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine. Cell Death and Disease, 2(5).
http://dx.doi.org/10.1038/cddis.2011.44
---------- CHICAGO ----------
Muglia, C., Mercer, N., Toscano, M.A., Schattner, M., Pozner, R., Cerliani, J.P., et al. "The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine" . Cell Death and Disease 2, no. 5 (2011).
http://dx.doi.org/10.1038/cddis.2011.44
---------- MLA ----------
Muglia, C., Mercer, N., Toscano, M.A., Schattner, M., Pozner, R., Cerliani, J.P., et al. "The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine" . Cell Death and Disease, vol. 2, no. 5, 2011.
http://dx.doi.org/10.1038/cddis.2011.44
---------- VANCOUVER ----------
Muglia, C., Mercer, N., Toscano, M.A., Schattner, M., Pozner, R., Cerliani, J.P., et al. The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine. Cell Death Dis. 2011;2(5).
http://dx.doi.org/10.1038/cddis.2011.44