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Abstract:

GTSE1 over-expression has been reported as a potential marker for metastasis in various types of malignancies, including breast cancer. Despite this, the transcriptional regulation of this protein and the causes of its misregulation in tumors remain largely unknown. The aims of this work were to elucidate how GTSE1 is regulated at the transcriptional level and to clarify the mechanism underlying GTSE1-dependent cell functions in triple-negative breast cancer (TNBC). Here, we identified GTSE1 as a novel target gene of the TEAD4 transcription factor, highlighting a role for the YAP and TAZ coactivators in the transcriptional regulation of GTSE1. Moreover, we found that TEAD4 controls the formation of cell protrusions required for cell migration through GTSE1, unveiling a relevant effector role for this protein in the TEAD-dependent cellular functions and confirming TEAD4 role in promoting invasion and metastasis in breast cancer. Finally, we highlighted a role for the pRb-E2F1 pathway in the control of GTSE1 transcription and observed that treatment with drugs targeting the pRb-E2F1 or YAP/ TAZ-TEAD pathways dramatically downregulated the expression levels of GTSE1 and of other genes involved in the formation of metastasis, suggesting their potential use in the treatment of TNBC. © Stelitano et al.

Registro:

Documento: Artículo
Título:GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models
Autor:Stelitano, D.; Leticia, Y.P.; Dalla, E.; Monte, M.; Piazza, S.; Schneider, C.
Filiación:Laboratorio Nazionale del Consorzio Interuniversitario per le Biotecnologie (L.N.CIB), Trieste, Italy
Laboratorio de Oncología Molecular, Departamento de Química Biológica and IQUIBICEN-UBA/CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina
Bioinformatics Core facility, Centre for Integrative Biology, University of Trento (CIBIO), Trento, Italy
Dipartimento di Scienze Biomediche e Biologiche (DSMB), Università degli Studi di Udine, Udine, Italy
Palabras clave:Cell protrusions; E2F1; GTSE1; TEAD4; YAP/TAZ; GTSE1 protein; messenger RNA; regulator protein; retinoblastoma protein; transcription factor; transcription factor E2F1; transcription factor HMGA1; transcription factor TAZ; transcription factor TEAD4; transcription factor YAP; unclassified drug; Article; cell invasion; cell migration; cell protrusion; cell structure; controlled study; G2 and S phase expressed 1 gene; gene; gene expression regulation; human; major clinical study; MDA-MB-157 cell line; MDA-MB-231 cell line; metastasis; mevalonate pathway; promoter region; signal transduction; transcription regulation; triple negative breast cancer
Año:2017
Volumen:8
Número:40
Página de inicio:67422
Página de fin:67438
Título revista:Oncotarget
Título revista abreviado:Oncotarget
ISSN:19492553
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano

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Citas:

---------- APA ----------
Stelitano, D., Leticia, Y.P., Dalla, E., Monte, M., Piazza, S. & Schneider, C. (2017) . GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models. Oncotarget, 8(40), 67422-67438.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano [ ]
---------- CHICAGO ----------
Stelitano, D., Leticia, Y.P., Dalla, E., Monte, M., Piazza, S., Schneider, C. "GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models" . Oncotarget 8, no. 40 (2017) : 67422-67438.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano [ ]
---------- MLA ----------
Stelitano, D., Leticia, Y.P., Dalla, E., Monte, M., Piazza, S., Schneider, C. "GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models" . Oncotarget, vol. 8, no. 40, 2017, pp. 67422-67438.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano [ ]
---------- VANCOUVER ----------
Stelitano, D., Leticia, Y.P., Dalla, E., Monte, M., Piazza, S., Schneider, C. GTSE1: A novel TEAD4-E2F1 target gene involved in cell protrusions formation in triple-negative breast cancer cell models. Oncotarget. 2017;8(40):67422-67438.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v8_n40_p67422_Stelitano [ ]