Artículo

Recouvreux, M.S.; Grasso, E.N.; Echeverria, P.C.; Rocha-Viegas, L.; Castilla, L.H.; Schere-Levy, C.; Tocci, J.M.; Kordon, E.C.; Rubinstein, N. "RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes" (2016) Oncotarget. 7(6):6552-6565
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Abstract:

Runx1 participation in epithelial mammary cells is still under review. Emerging data indicates that Runx1 could be relevant for breast tumor promotion. However, to date no studies have specifically evaluated the functional contribution of Runx1 to control gene expression in mammary epithelial tumor cells. It has been described that Runx1 activity is defined by protein context interaction. Interestingly, Foxp3 is a breast tumor suppressor gene. Here we show that endogenous Runx1 and Foxp3 physically interact in normal mammary cells and this interaction blocks Runx1 transcriptional activity. Furthermore we demonstrate that Runx1 is able to bind to R-spondin 3 (RSPO3) and Gap Junction protein Alpha 1 (GJA1) promoters. This binding upregulates Rspo3 oncogene expression and downregulates GJA1 tumor suppressor gene expression in a Foxp3-dependent manner. Moreover, reduced Runx1 transcriptional activity decreases tumor cell migration properties. Collectively, these data provide evidence of a new mechanism for breast tumor gene expression regulation, in which Runx1 and Foxp3 physically interact to control mammary epithelial cell gene expression fate. Our work suggests for the first time that Runx1 could be involved in breast tumor progression depending on Foxp3 availability.

Registro:

Documento: Artículo
Título:RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes
Autor:Recouvreux, M.S.; Grasso, E.N.; Echeverria, P.C.; Rocha-Viegas, L.; Castilla, L.H.; Schere-Levy, C.; Tocci, J.M.; Kordon, E.C.; Rubinstein, N.
Filiación:Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, UBA, Buenos Aires, Argentina
Departamento de Química Biológica, UBA, Buenos Aires, Argentina
Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, United States
Department of Biologie Cellulaire, Universite de Geneve Sciences III, Geneve, Switzerland
MS Recouvreux, Oncology Institute 'Angel H Roffo', Buenos Aires, Argentina
EN Grasso, Immunopharmacology Lab, IQUIBICEN-CONICET, FCEN-UBA, Buenos Aires, Argentina
Palabras clave:Foxp3; Gene expression regulation; GJA1; Rspo3; Runx1; transcription factor FOXP3; transcription factor RUNX1; connexin 43; forkhead transcription factor; FOXP3 protein, human; GJA1 protein, human; RSPO3 protein, human; RUNX1 protein, human; thrombospondin; transcription factor RUNX1; animal cell; Article; breast cancer; breast cell; breast epithelium cell; cell migration; controlled study; down regulation; FOXP3 gene; gene activity; gene expression; gene interaction; genetic transcription; GJA1 gene; in vitro study; nonhuman; pathogenesis; promoter region; RUNX1 gene; tumor suppressor gene; upregulation; adenocarcinoma; animal; apoptosis; Bagg albino mouse; breast tumor; cell motion; cell proliferation; chromatin immunoprecipitation; confocal microscopy; drug screening; enzyme immunoassay; female; fluorescence microscopy; fluorescent antibody technique; gene expression regulation; genetics; human; immunoprecipitation; metabolism; mouse; pathology; tumor cell culture; Western blotting; wound healing; Adenocarcinoma; Animals; Apoptosis; Blotting, Western; Breast Neoplasms; Cell Movement; Cell Proliferation; Chromatin Immunoprecipitation; Connexin 43; Core Binding Factor Alpha 2 Subunit; Female; Fluorescent Antibody Technique; Forkhead Transcription Factors; Gene Expression Regulation, Neoplastic; Humans; Immunoenzyme Techniques; Immunoprecipitation; Mice; Mice, Inbred BALB C; Microscopy, Confocal; Microscopy, Fluorescence; Promoter Regions, Genetic; Thrombospondins; Tumor Cells, Cultured; Wound Healing; Xenograft Model Antitumor Assays
Año:2016
Volumen:7
Número:6
Página de inicio:6552
Página de fin:6565
DOI: http://dx.doi.org/10.18632/oncotarget.6771
Título revista:Oncotarget
Título revista abreviado:Oncotarget
ISSN:19492553
CAS:Connexin 43; Core Binding Factor Alpha 2 Subunit; Forkhead Transcription Factors; FOXP3 protein, human; GJA1 protein, human; RSPO3 protein, human; RUNX1 protein, human; Thrombospondins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19492553_v7_n6_p6552_Recouvreux

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Citas:

---------- APA ----------
Recouvreux, M.S., Grasso, E.N., Echeverria, P.C., Rocha-Viegas, L., Castilla, L.H., Schere-Levy, C., Tocci, J.M.,..., Rubinstein, N. (2016) . RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes. Oncotarget, 7(6), 6552-6565.
http://dx.doi.org/10.18632/oncotarget.6771
---------- CHICAGO ----------
Recouvreux, M.S., Grasso, E.N., Echeverria, P.C., Rocha-Viegas, L., Castilla, L.H., Schere-Levy, C., et al. "RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes" . Oncotarget 7, no. 6 (2016) : 6552-6565.
http://dx.doi.org/10.18632/oncotarget.6771
---------- MLA ----------
Recouvreux, M.S., Grasso, E.N., Echeverria, P.C., Rocha-Viegas, L., Castilla, L.H., Schere-Levy, C., et al. "RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes" . Oncotarget, vol. 7, no. 6, 2016, pp. 6552-6565.
http://dx.doi.org/10.18632/oncotarget.6771
---------- VANCOUVER ----------
Recouvreux, M.S., Grasso, E.N., Echeverria, P.C., Rocha-Viegas, L., Castilla, L.H., Schere-Levy, C., et al. RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes. Oncotarget. 2016;7(6):6552-6565.
http://dx.doi.org/10.18632/oncotarget.6771