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Abstract:

Growth and polymer synthesis were studied in a recombinant E. coli strain carrying phaBAC and phaP of Azotobacter sp. strain FA8 using different carbon sources and oxygen availability conditions. The results obtained with glucose or glycerol were completely different, demonstrating that the metabolic routes leading to the synthesis of the polymer when using glycerol do not respond to environmental conditions such as oxygen availability in the same way as they do when other substrates, such as glucose, are used. When cells were grown in a bioreactor using glucose the amount of polymer accumulated at low aeration was reduced by half when compared to high aeration, while glycerol cultures produced at low aeration almost twice the amount of polymer synthesized at the higher aeration condition. The synthesis of other metabolic products, such as ethanol, lactate, formate and acetate, were also affected by both the carbon source used and aeration conditions. In glucose cultures, lactate and formate production increased in low agitation compared to high agitation, while poly(3-hydroxybutyrate) synthesis decreased. In glycerol cultures, the amount of acids produced also increased when agitation was lowered, but carbon flow was mostly redirected towards ethanol and poly(3-hydroxybutyrate). These results indicated that carbon partitioning differed depending on both carbon source and oxygen availability, and that aeration conditions had different effects on the synthesis of the polymer and other metabolic products when glucose or glycerol were used. © 2010 Landes Bioscience.

Registro:

Documento: Artículo
Título:Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli
Autor:Nikel, P.I.; de Almeida, A.; Giordano, A.M.; Pettinari, M.J.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín, Buenos Aires, Argentina
Palabras clave:Aeration; Carbon partitioning; Escherichia coli; Glucose; Glycerol; Oxygen availability; Poly(3-hydroxybutyrate); glycerol; poly(3 hydroxybutyric acid); aeration; article; bacterial growth; bacterial metabolism; bacterium culture; carbon source; Escherichia coli; nonhuman; oxidation reduction reaction; synthesis; Carbon; Escherichia coli; Glucose; Glycerol; Hydroxybutyrates; Oxidation-Reduction; Oxygen; Polyesters; Azotobacter; Escherichia coli
Año:2010
Volumen:1
Número:4
Página de inicio:291
Página de fin:295
Título revista:Bioengineered Bugs
Título revista abreviado:Bioeng. Bugs
ISSN:19491018
CAS:glycerol, 56-81-5; poly(3 hydroxybutyric acid), 26063-00-3; Carbon, 7440-44-0; Glucose, 50-99-7; Glycerol, 56-81-5; Hydroxybutyrates; Oxygen, 7782-44-7; Polyesters; poly-beta-hydroxybutyrate, 26063-00-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19491018_v1_n4_p291_Nikel

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Citas:

---------- APA ----------
Nikel, P.I., de Almeida, A., Giordano, A.M. & Pettinari, M.J. (2010) . Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli. Bioengineered Bugs, 1(4), 291-295.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19491018_v1_n4_p291_Nikel [ ]
---------- CHICAGO ----------
Nikel, P.I., de Almeida, A., Giordano, A.M., Pettinari, M.J. "Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli" . Bioengineered Bugs 1, no. 4 (2010) : 291-295.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19491018_v1_n4_p291_Nikel [ ]
---------- MLA ----------
Nikel, P.I., de Almeida, A., Giordano, A.M., Pettinari, M.J. "Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli" . Bioengineered Bugs, vol. 1, no. 4, 2010, pp. 291-295.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19491018_v1_n4_p291_Nikel [ ]
---------- VANCOUVER ----------
Nikel, P.I., de Almeida, A., Giordano, A.M., Pettinari, M.J. Redox driven metabolic tuning: Carbon source and aeration affect synthesis of poly(3-hydroxybutyrate) in Escherichia coli. Bioeng. Bugs. 2010;1(4):291-295.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19491018_v1_n4_p291_Nikel [ ]