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Abstract:

We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution. © 2013 American Chemical Society.

Registro:

Documento: Artículo
Título:Two-photon optical interrogation of individual dendritic spines with caged dopamine
Autor:Araya, R.; Andino-Pavlovsky, V.; Yuste, R.; Etchenique, R.
Filiación:Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pabellón 2, AR1428EHA Buenos Aires, Argentina
Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY 10027, United States
Département de Physiologie, Université de Montréal, 2960 Chemin de la Tour, Montréal, QC H3T 1J4, Canada
Palabras clave:caged compound; Dopamine; ruthenium; two-photon; bipyridine; calcium; dopamine; dopamine receptor; ruthenium; animal cell; article; brain slice; dendritic spine; fluorescence imaging; infrared photography; mouse; nonhuman; photochemistry; photon; priority journal; protein expression; quantum yield; Animals; Calcium Isotopes; Dendritic Spines; Dopamine; Mice; Photolysis; Photons; Prefrontal Cortex; Receptors, Dopamine; Ruthenium
Año:2013
Volumen:4
Número:8
Página de inicio:1163
Página de fin:1167
DOI: http://dx.doi.org/10.1021/cn4000692
Título revista:ACS Chemical Neuroscience
Título revista abreviado:ACS Chem. Neurosci.
ISSN:19487193
CODEN:ACNCD
CAS:bipyridine, 37275-48-2; calcium, 14092-94-5, 7440-70-2; dopamine, 51-61-6, 62-31-7; ruthenium, 7440-18-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19487193_v4_n8_p1163_Araya

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Citas:

---------- APA ----------
Araya, R., Andino-Pavlovsky, V., Yuste, R. & Etchenique, R. (2013) . Two-photon optical interrogation of individual dendritic spines with caged dopamine. ACS Chemical Neuroscience, 4(8), 1163-1167.
http://dx.doi.org/10.1021/cn4000692
---------- CHICAGO ----------
Araya, R., Andino-Pavlovsky, V., Yuste, R., Etchenique, R. "Two-photon optical interrogation of individual dendritic spines with caged dopamine" . ACS Chemical Neuroscience 4, no. 8 (2013) : 1163-1167.
http://dx.doi.org/10.1021/cn4000692
---------- MLA ----------
Araya, R., Andino-Pavlovsky, V., Yuste, R., Etchenique, R. "Two-photon optical interrogation of individual dendritic spines with caged dopamine" . ACS Chemical Neuroscience, vol. 4, no. 8, 2013, pp. 1163-1167.
http://dx.doi.org/10.1021/cn4000692
---------- VANCOUVER ----------
Araya, R., Andino-Pavlovsky, V., Yuste, R., Etchenique, R. Two-photon optical interrogation of individual dendritic spines with caged dopamine. ACS Chem. Neurosci. 2013;4(8):1163-1167.
http://dx.doi.org/10.1021/cn4000692