Artículo
Laufer, N.; Ojeda, D.; Polo, M.L.; Martinez, A.; Pérez, H.; Turk, G.; Cahn, P.; Zwirner, N.W.; Quarleri, J. "CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C viruscoinfected patients" (2017) World Journal of Hepatology. 9(25):1073-1080
Estamos trabajando para incorporar este artículo al repositorio
Consulte la política de Acceso Abierto del editor
Abstract:
AIM To characterize peripheral blood natural killer (NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus (HIV)/ hepatitis C virus (HCV) coinfected patients. METHODS Peripheral mononuclear cells from 24 HIV/HCV (HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: Anti- CD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), anti- NKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L (PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-FasL(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using FlowJo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained. RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was (6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls (G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1 (G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035). CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. © The Author(s) 2017.
Registro:
| Documento: | Artículo |
| Título: | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C viruscoinfected patients |
| Autor: | Laufer, N.; Ojeda, D.; Polo, M.L.; Martinez, A.; Pérez, H.; Turk, G.; Cahn, P.; Zwirner, N.W.; Quarleri, J. |
| Filiación: | CONICET, Instituto Investigaciones Biomédicas en Retrovirus y SIDA INBIRS, Universidad de Buenos Aires, Buenos Aires, C1121ABG, Argentina Instituto INBIRS, UBA-CONICET, Buenos Aires, C1121ABG, Argentina Hospital Juan A Fernández, Buenos Aires, C1121ABG, Argentina Fundación Huésped, Buenos Aires, C1121ABG, Argentina Laboratorio de Fisiopatología de la Inmunidad, Innata Instituto de Biología y Medicina Experimental, Buenos Aires, C1121ABG, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1121ABG, Argentina |
| Palabras clave: | Biomarker; CD4+ T cell; Fibrosis; Human immunodeficiency virus/hepatitis C virus-coinfection; Natural killer cells |
| Año: | 2017 |
| Volumen: | 9 |
| Número: | 25 |
| Página de inicio: | 1073 |
| Página de fin: | 1080 |
| DOI: | http://dx.doi.org/10.4254/wjh.v9.i25.1073 |
| Título revista: | World Journal of Hepatology |
| Título revista abreviado: | World J. Hepatol. |
| ISSN: | 19485182 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19485182_v9_n25_p1073_Laufer |
Referencias:
- Gower, E., Estes, C., Blach, S., Razavi-Shearer, K., Razavi, H., Global epidemiology and genotype distribution of the hepatitis C virus infection (2014) J Hepatol, 61, pp. S45-S57. , [PMID: 25086286]
- (2015) How AIDS changes everything-MDG 6: 15 lessons of hope from the AIDS response, , UNAIDS
- Platt, L., Easterbrook, P., Gower, E., McDonald, B., Sabin, K., McGowan, C., Yanny, I., Vickerman, P., Prevalence and burden of HCV co-infection in people living with HIV: a global systematic review and meta-analysis (2016) Lancet Infect Dis, 16, pp. 797-808. , [PMID: 26922272]
- Zoulim, F., Liang, T.J., Gerbes, A.L., Aghemo, A., Deuffic-Burban, S., Dusheiko, G., Fried, M.W., Wiktor, S., Hepatitis C virus treatment in the real world: optimising treatment and access to therapies (2015) Gut, 64, pp. 1824-1833. , [PMID: 26449729]
- Actualización de las Recomendaciones para el Tratamiento de la Hepatitis Crónica por Virus C 2014, , URL:http://www.aaeeh.org.ar/neweb/docs/AAEEH-Guia-tto-Hepatitis-C-Cronica.pdf, Asociación Argentina para el Estudio de las Enfermedades del Hígado
- EASL Recommendations on Treatment of Hepatitis C 2015 (2015) J Hepatol, 63, pp. 199-236. , [PMID: 25911336]
- Yi, H.S., Eun, H.S., Lee, Y.S., Jung, J.Y., Park, S.H., Park, K.G., Choi, H.S., Jeong, W.I., Treatment with 4-methylpyrazole modulated stellate cells and natural killer cells and ameliorated liver fibrosis in mice (2015) PLoS One, 10. , [PMID: 26024318]
- Goeser, F., Glässner, A., Kokordelis, P., Wolter, F., Lutz, P., Kaczmarek, D.J., Schwarze-Zander, C., Nattermann, J., HIV mono-infection is associated with an impaired anti-hepatitis C virus activity of natural killer cells (2016) AIDS, 30, pp. 355-363. , [PMID: 26558728]
- Howell, J., Visvanathan, K., The role of natural killer cells in hepatitis C infection (2013) Antivir Ther, 18, pp. 853-865. , [PMID: 23559549]
- Girart, M.V., Fuertes, M.B., Domaica, C.I., Rossi, L.E., Zwirner, N.W., Engagement of TLR3, TLR7, and NKG2D regulate IFN-gamma secretion but not NKG2D-mediated cytotoxicity by human NK cells stimulated with suboptimal doses of IL-12 (2007) J Immunol, 179, pp. 3472-3479. , [PMID: 17804388]
- Zwirner, N.W., Domaica, C.I., Cytokine regulation of natural killer cell effector functions (2010) Biofactors, 36, pp. 274-288. , [PMID: 20623510]
- Eisenberg, E., Konopniki, M., Veitsman, E., Kramskay, R., Gaitini, D., Baruch, Y., Prevalence and characteristics of pain induced by percutaneous liver biopsy (2003) Anesth Analg, 96, pp. 1392-1396. , table of contents [PMID: 12707140]
- Regev, A., Berho, M., Jeffers, L.J., Milikowski, C., Molina, E.G., Pyrsopoulos, N.T., Feng, Z.Z., Schiff, E.R., Sampling error and intraobserver variation in liver biopsy in patients with chronic HCV infection (2002) Am J Gastroenterol, 97, pp. 2614-2618. , [PMID: 12385448]
- EASL-ALEH Clinical Practice Guidelines: Non-invasive tests for evaluation of liver disease severity and prognosis (2015) J Hepatol, 63, pp. 237-264. , [PMID: 25911335]
- Moretta, L., Montaldo, E., Vacca, P., Del Zotto, G., Moretta, F., Merli, P., Locatelli, F., Mingari, M.C., Human natural killer cells: origin, receptors, function, and clinical applications (2014) Int Arch Allergy Immunol, 164, pp. 253-264. , [PMID: 25323661]
- Nielsen, C.M., White, M.J., Goodier, M.R., Riley, E.M., Functional Significance of CD57 Expression on Human NK Cells and Relevance to Disease (2013) Front Immunol, 4, p. 422. , [PMID: 24367364]
- Glässner, A., Eisenhardt, M., Kokordelis, P., Krämer, B., Wolter, F., Nischalke, H.D., Boesecke, C., Nattermann, J., Impaired CD4+ T cell stimulation of NK cell anti-fibrotic activity may contribute to accelerated liver fibrosis progression in HIV/HCV patients (2013) J Hepatol, 59, pp. 427-433. , [PMID: 23665286]
- Yi, H.S., Jeong, W.I., Interaction of hepatic stellate cells with diverse types of immune cells: foe or friend? (2013) J Gastroenterol Hepatol, 28, pp. 99-104. , [PMID: 23855303]
- Glässner, A., Eisenhardt, M., Krämer, B., Körner, C., Coenen, M., Sauerbruch, T., Spengler, U., Nattermann, J., NK cells from HCVinfected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL-and NKG2Ddependent manner (2012) Lab Invest, 92, pp. 967-977. , [PMID: 22449797]
- Bhardwaj, S., Ahmad, F., Wedemeyer, H., Cornberg, M., Schulze Zur Wiesch, J., van Lunzen, J., Sarin, S.K., Meyer-Olson, D., Increased CD56(bright) NK cells in HIV-HCV co-infection and HCV mono-infection are associated with distinctive alterations of their phenotype (2016) Virol J, 13, p. 67. , [PMID: 27091211]
- Ahlenstiel, G., Titerence, R.H., Koh, C., Edlich, B., Feld, J.J., Rotman, Y., Ghany, M.G., Rehermann, B., Natural killer cells are polarized toward cytotoxicity in chronic hepatitis C in an interferon-alfa-dependent manner (2010) Gastroenterology, 138, pp. 325-335.e1-e2. , [PMID: 19747917]
- Serti, E., Chepa-Lotrea, X., Kim, Y.J., Keane, M., Fryzek, N., Liang, T.J., Ghany, M., Rehermann, B., Successful Interferon-Free Therapy of Chronic Hepatitis C Virus Infection Normalizes Natural Killer Cell Function (2015) Gastroenterology, 149, pp. 190-200 and e2. , [PMID: 25754160]
Citas:
---------- APA ----------
Laufer, N., Ojeda, D., Polo, M.L., Martinez, A., Pérez, H., Turk, G., Cahn, P.,..., Quarleri, J. (2017) . CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C viruscoinfected patients. World Journal of Hepatology, 9(25), 1073-1080.http://dx.doi.org/10.4254/wjh.v9.i25.1073
---------- CHICAGO ----------
Laufer, N., Ojeda, D., Polo, M.L., Martinez, A., Pérez, H., Turk, G., et al. "CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C viruscoinfected patients" . World Journal of Hepatology 9, no. 25 (2017) : 1073-1080.http://dx.doi.org/10.4254/wjh.v9.i25.1073
---------- MLA ----------
Laufer, N., Ojeda, D., Polo, M.L., Martinez, A., Pérez, H., Turk, G., et al. "CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C viruscoinfected patients" . World Journal of Hepatology, vol. 9, no. 25, 2017, pp. 1073-1080.http://dx.doi.org/10.4254/wjh.v9.i25.1073
---------- VANCOUVER ----------
Laufer, N., Ojeda, D., Polo, M.L., Martinez, A., Pérez, H., Turk, G., et al. CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C viruscoinfected patients. World J. Hepatol. 2017;9(25):1073-1080.http://dx.doi.org/10.4254/wjh.v9.i25.1073
