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Abstract:

The range of action of intracellular messengers is determined by their rates of diffusion and degradation. Previous measurements in oocyte cytoplasmic extracts indicated that the Ca 2+ -liberating secondmessenger inositol trisphosphate (IP 3 ) diffuses with a coefficient (∼280 mm2 s -1 ) similar to that in water, corresponding to a range of action of ∼25 mm. Consequently, IP 3 is generally considered a "global" cellular messenger. We reexamined this issue by measuring local IP 3 -evoked Ca 2+ puffs to monitor IP 3 diffusing from spot photorelease in neuroblastoma cells. Fitting these data by numerical simulations yielded a diffusion coefficient (≤10 mm 2 s -1 ) about 30-fold slower than that previously reported. We propose that diffusion of IP 3 in mammalian cells is hindered by binding to immobile, functionally inactive receptors that were diluted in oocyte extracts. The predicted range of action of IP 3 (<5 mm) is thus smaller than the size of typical mammalian cells, indicating that IP 3 should better be considered as a local rather than a global cellular messenger. 2016 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.

Registro:

Documento: Artículo
Título:Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action
Autor:Dickinson, G.D.; Ellefsen, K.L.; Dawson, S.P.; Pearson, J.E.; Parker, I.
Filiación:Department of Neurobiology and Behavior, University of California, Irvine, CA 92697, United States
Departamento de Física, FCEN-UBA, IFIBA, CONICET, Buenos Aires, Argentina
Theoretical Biology and Biophysics, T-10 MS K710, Los Alamos National Laboratory, Los Alamos, NM 87545, United States
Department of Physiology and Biophysics, University of California, Irvine, CA 92697, United States
Palabras clave:calcium ion; inositol trisphosphate; calcium; inositol 1,4,5 trisphosphate; Article; binding site; cell function; controlled study; COS 7 cell line; cytoplasm; cytosol; degradation; dendrite; diffusion coefficient; dissociation constant; gap junction; human; human cell; neuroblastoma cell; oocyte; photolysis; photon; priority journal; calcium signaling; cytoplasm; metabolism; neuroblastoma; tumor cell line; Calcium; Calcium Signaling; Cell Line, Tumor; Cytoplasm; Humans; Inositol 1,4,5-Trisphosphate; Neuroblastoma
Año:2016
Volumen:9
Número:453
DOI: http://dx.doi.org/10.1126/scisignal.aag1625
Título revista:Science Signaling
Título revista abreviado:Sci. Signal.
ISSN:19450877
CAS:calcium ion, 14127-61-8; inositol trisphosphate, 27121-73-9; calcium, 7440-70-2, 14092-94-5; inositol 1,4,5 trisphosphate, 85166-31-0, 88269-39-0; Calcium; Inositol 1,4,5-Trisphosphate
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19450877_v9_n453_p_Dickinson

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Citas:

---------- APA ----------
Dickinson, G.D., Ellefsen, K.L., Dawson, S.P., Pearson, J.E. & Parker, I. (2016) . Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action. Science Signaling, 9(453).
http://dx.doi.org/10.1126/scisignal.aag1625
---------- CHICAGO ----------
Dickinson, G.D., Ellefsen, K.L., Dawson, S.P., Pearson, J.E., Parker, I. "Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action" . Science Signaling 9, no. 453 (2016).
http://dx.doi.org/10.1126/scisignal.aag1625
---------- MLA ----------
Dickinson, G.D., Ellefsen, K.L., Dawson, S.P., Pearson, J.E., Parker, I. "Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action" . Science Signaling, vol. 9, no. 453, 2016.
http://dx.doi.org/10.1126/scisignal.aag1625
---------- VANCOUVER ----------
Dickinson, G.D., Ellefsen, K.L., Dawson, S.P., Pearson, J.E., Parker, I. Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action. Sci. Signal. 2016;9(453).
http://dx.doi.org/10.1126/scisignal.aag1625