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Abstract:

Herein, we report on the implementation of photofunctional surfaces for the investigation of cellular responses by means of quantitative fluorescence microscopy. The developed substrates are able to produce reactive oxygen species under the fluorescence microscope upon irradiation with visible light, and the behavior of cells grown on these surfaces can be consequently investigated in situ and in real time. Moreover, a suitable methodology is presented to simultaneously monitor phototriggered morphological changes and the associated molecular pathways with spatiotemporal resolution employing time-resolved fluorescence anisotropy at the single cell level. The results showed that morphological changes can be complemented with a quantitative evaluation of the associated molecular signaling cascades for the unambiguous assignment of reactive oxygen species-related photoinduced apoptosis. Indeed, similar phenotypes are associated with different cellular processes. Our methodology facilitates the in vitro design and evaluation of photosensitizers for the treatment of cancer and infectious diseases with the aid of functional fluorescence microscopy. (Chemical Presented). © 2015 American Chemical Society.

Registro:

Documento: Artículo
Título:Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution
Autor:Stegemann, L.; Schuermann, K.C.; Strassert, C.A.; Grecco, H.E.
Filiación:Physikalisches Institut, Center for Nanotechnology (CeNTech), Westfälische Wilhelms-Universität Münster, Heisenbergstraße 11, Münster, D-48149, Germany
Department of Systemic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn Straße 11, Dortmund, D-44227, Germany
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:apoptosis; caspase activity; cell death; necrosis; photoactive surfaces; phototherapy; quantitative functional microscopy; reactive oxygen species; Cell death; Cells; Cytology; Diseases; Fluorescence; Fluorescence microscopy; Mobile security; Morphology; Oxygen; Photosensitizers; Caspases; necrosis; Phototherapy; Quantitative evaluation; Quantitative fluorescence microscopy; Reactive oxygen species; Spatio-temporal resolution; Time resolved fluorescence anisotropy; Molecular oxygen; nanoparticle; reactive oxygen metabolite; chemistry; fluorescence microscopy; HeLa cell line; human; light; metabolism; molecular imaging; photochemistry; procedures; radiation response; spatiotemporal analysis; HeLa Cells; Humans; Light; Microscopy, Fluorescence; Molecular Imaging; Nanoparticles; Photochemistry; Reactive Oxygen Species; Spatio-Temporal Analysis
Año:2015
Volumen:7
Número:10
Página de inicio:5944
Página de fin:5949
DOI: http://dx.doi.org/10.1021/acsami.5b00130
Título revista:ACS Applied Materials and Interfaces
Título revista abreviado:ACS Appl. Mater. Interfaces
ISSN:19448244
CAS:Reactive Oxygen Species
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19448244_v7_n10_p5944_Stegemann

Referencias:

  • Dolmans, D.E.G.J., Fukumura, D., Jain, R.K., Photodynamic Therapy for Cancer (2003) Nat. Rev. Cancer, 3, pp. 380-387
  • Circu, M.L., Aw, T.Y., Reactive Oxygen Species, Cellular Redox Systems, and Apoptosis (2010) Free Radic. Biol. Med., 48, pp. 749-762
  • Lavie, G., Kaplinsky, C., Toren, A., Aizman, I., Meruelo, D., Mazur, Y., Mandel, M., A Photodynamic Pathway to Apoptosis and Necrosis Induced by Dimethyl tetrahydroxyhelianthrone and Hypericin in Leukaemic Cells: Possible Relevance to Photodynamic Therapy (1999) Br. J. Cancer, 79, pp. 423-432
  • Castano, A.P., Demidova, T.N., Hamblin, M.R., Mechanisms in Photodynamic Therapy: Part One-Photosensitizers, Photochemistry and Cellular Localization (2004) Photodiagnosis Photodyn. Ther., 1, pp. 279-293
  • Kamarulzaman, E.E., Benachour, H., Barberi-Heyob, M., Frochot, C., Wahab, H.A., Guillemin, F., Vanderesse, R., Vascular-Targeted Photodynamic Therapy (VTP) (2011) Advances in Cancer Therapy, , Gali-Muhtasib, H., Ed.; In Tech: Rijeka, Croatia
  • Wang, W., Moriyama, L.T., Bagnato, V.S., Photodynamic Therapy Induced Vascular Damage: An Overview of Experimental PDT (2013) Laser Phys. Lett., 10, p. 023001
  • Chen, B., Pogue, B.W., Hoopes, P.J., Hasan, T., Vascular and Cellular Targeting for Photodynamic Therapy (2006) Crit. Rev. Eukaryotic Gene Expression, 16, pp. 279-305
  • Rhee, S., H2O2, a Necessary Evil for Cell Signaling (2006) Science, 312, pp. 1882-1883. , G
  • Rhee, S.G., Bae, Y.S., Lee, S.-R., Kwon, J., Hydrogen Peroxide: A Key Messenger that Modulates Protein Phosphorylation Through Cysteine Oxidation (2000) Sci. Signaling, 2000, p. pe1
  • Tonks, N.K., Redox Redux: Revisiting PTPs and the Control of Cell Signaling (2005) Cell, 121, pp. 667-670
  • Ogilby, P.R., Singlet Oxygen: There is Indeed Something New under the Sun (2010) Chem. Soc. Rev., 39, pp. 3181-3209
  • Tischer, C., Bastiaens, P.I.H., Lateral Phosphorylation Propagation: An Aspect of Feedback Signalling? (2003) Nat. Rev. Mol. Cell Biol., 4, pp. 971-975
  • Pimenta, F.M., Jensen, R.L., Holmegaard, L., Esipova, T.V., Westberg, M., Breitenbach, T., Ogilby, P.R., Singlet-Oxygen-Mediated Cell Death Using Spatially-Localized Two-Photon Excitation of an Extracellular Sensitizer (2012) J. Phys. Chem. B, 116, pp. 10234-10246
  • Klotz, L.O., Pellieux, C., Briviba, K., Pierlot, C., Aubry, J.M., Sies, H., Mitogen-Activated Protein Kinase (p38-, JNK-, ERK-) Activation Pattern Induced by Extracellular and Intracellular Singlet Oxygen and UVA (1999) Eur. J. Biochem., 260, pp. 917-922
  • Harpur, A.G., Wouters, F.S., Bastiaens, P.I.H., Imaging FRET between Spectrally Similar GFP Molecules in Single Cells (2001) Nat. Biotechnol., 19, pp. 167-169
  • Kamentsky, L., Jones, T.R., Fraser, A., Bray, M.-A., Logan, D.J., Madden, K.L., Ljosa, V., Carpenter, A.E., Improved Structure, Function and Compatibility for CellProfiler: Modular High-Throughput Image Analysis Software (2011) Bioinformatics, 27, pp. 1179-1180
  • Lewis, G.N., Goldschmid, O., Magel, T.T., Bigeleisen, J., Dimeric and Other Forms of Methylene Blue: Absorption and Fluorescence of the Pure Monomer (1943) J. Am. Chem. Soc., 65, pp. 1150-1154
  • Mirenda, M., Strassert, C.A., Dicelio, L.E., San Román, E., Dye-Polyelectrolyte Layer-by-Layer Self-Assembled Materials: Molecular Aggregation, Structural Stability, and Singlet Oxygen Photogeneration (2010) ACS Appl. Mater. Interfaces, 2, pp. 1556-1560
  • Puck, T.T., Marcus, P.I., Cieciura, S.J., Clonal Growth of Mammalian Cells in Vitro; Growth Characteristics of Colonies from Single HeLa Cells with and without a Feeder Layer (1956) J. Exp. Med., 103, pp. 273-283
  • Stockert, J.C., Juarranz, A., Villanueva, A., Cañete, M., Photodynamic Damage to HeLa Cell Microtubules Induced by Thiazine Dyes (1996) Cancer Chemother. Pharmacol., 39, pp. 167-169
  • Haendeler, J., Tischler, V., Hoffmann, J., Zeiher, A.M., Dimmeler, S., Low Doses of Reactive Oxygen Species Protect Endothelial Cells from Apoptosis by Increasing Thioredoxin-1 Expression (2004) FEBS Lett., 577, pp. 427-433
  • Wang, J.F., Zhang, X., Groopman, J.E., Activation of Vascular Endothelial Growth Factor Receptor-3 and Its Downstream Signaling Promote Cell Survival under Oxidative Stress (2004) J. Biol. Chem., 279, pp. 27088-27097

Citas:

---------- APA ----------
Stegemann, L., Schuermann, K.C., Strassert, C.A. & Grecco, H.E. (2015) . Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution. ACS Applied Materials and Interfaces, 7(10), 5944-5949.
http://dx.doi.org/10.1021/acsami.5b00130
---------- CHICAGO ----------
Stegemann, L., Schuermann, K.C., Strassert, C.A., Grecco, H.E. "Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution" . ACS Applied Materials and Interfaces 7, no. 10 (2015) : 5944-5949.
http://dx.doi.org/10.1021/acsami.5b00130
---------- MLA ----------
Stegemann, L., Schuermann, K.C., Strassert, C.A., Grecco, H.E. "Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution" . ACS Applied Materials and Interfaces, vol. 7, no. 10, 2015, pp. 5944-5949.
http://dx.doi.org/10.1021/acsami.5b00130
---------- VANCOUVER ----------
Stegemann, L., Schuermann, K.C., Strassert, C.A., Grecco, H.E. Photofunctional Surfaces for Quantitative Fluorescence Microscopy: Monitoring the Effects of Photogenerated Reactive Oxygen Species at Single Cell Level with Spatiotemporal Resolution. ACS Appl. Mater. Interfaces. 2015;7(10):5944-5949.
http://dx.doi.org/10.1021/acsami.5b00130