Artículo

Delcanale, P.; Galstyan, A.; Daniliuc, C.G.; Grecco, H.E.; Abbruzzetti, S.; Faust, A.; Viappiani, C.; Strassert, C.A. "Oxygen-Insensitive Aggregates of Pt(II) Complexes as Phosphorescent Labels of Proteins with Luminescence Lifetime-Based Readouts" (2018) ACS Applied Materials and Interfaces. 10(29):24361-24369
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Abstract:

The synthesis and photophysical properties of a tailored Pt(II) complex are presented. The phosphorescence of its monomeric species in homogeneous solutions is quenched by interaction with the solvent and therefore absent even upon deoxygenation. However, aggregation-induced shielding from the environment and suppression of rotovibrational degrees of freedom trigger a phosphorescence turn-on that is not suppressed by molecular oxygen, despite possessing an excited-state lifetime ranging in the microsecond scale. Thus, the photoinduced production of reactive oxygen species is avoided by the suppression of diffusion-controlled Dexter-type energy transfer to triplet molecular oxygen. These aggregates emit with the characteristic green luminescence profile of monomeric complexes, indicating that Pt-Pt or excimeric interactions are negligible. Herein, we show that these aggregates can be used to label a model biomolecule (bovine serum albumin) with a microsecond-range luminescence. The protein stabilizes the aggregates, acting as a carrier in aqueous environments. Despite spectral overlaps, the green phosphorescence can be separated by time-gated detection from the dominant autofluorescence of the protein arising from a covalently bound green fluorophore that emits in the nanosecond range. Interestingly, the aggregates also acted as energy donors able to sensitize the emission of a fraction of the fluorophores bound to the protein. This resulted in a microsecond-range luminescence of the fluorescent acceptors and a shortening of the excited-state lifetime of the phosphorescent aggregates. The process that can be traced by a 1000-fold increase in the acceptor's lifetime mirrors the donor's triplet character. The implications for phosphorescence lifetime imaging are discussed. © 2018 American Chemical Society.

Registro:

Documento: Artículo
Título:Oxygen-Insensitive Aggregates of Pt(II) Complexes as Phosphorescent Labels of Proteins with Luminescence Lifetime-Based Readouts
Autor:Delcanale, P.; Galstyan, A.; Daniliuc, C.G.; Grecco, H.E.; Abbruzzetti, S.; Faust, A.; Viappiani, C.; Strassert, C.A.
Filiación:Dipartimento di Scienze Matematiche, Fisiche e Informatiche, Parco Area delle Scienze 7A, Parma, 43124, Italy
Physikalisches Institut and Center for Nanotechnology, Westfälische Wilhelms-Universität Münster, Heisenbergstraße 11, Münster, D-48149, Germany
Organisch-Chemisches Institut, Westfälische Wilhelms-Universität Münster, Corrensstraße 40, Münster, D-48149, Germany
Departamento de Física, FCEyN, UBA, IFIBA, CONICET, Pabellón 1, Ciudad Universitaria, Buenos Aires, 1428, Argentina
University Hospital Münster, European Institute for Molecular Imaging, Westfälische Wilhelms-Universität Münster, Waldeyerstraße 15, Münster, D-48149, Germany
Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science Technology (BIST), Barcelona, Spain
Palabras clave:donor-acceptor; energy transfer; labeling; photophysics; PLIM; Pt(II) complex; spectroscopy; time-gated spectroscopy; Aggregates; Binary alloys; Body fluids; Degrees of freedom (mechanics); Energy transfer; Excited states; Fluorophores; Labeling; Molecular oxygen; Phosphorescence; Proteins; Spectroscopy; Donor acceptors; Excited state lifetimes; Phosphorescence lifetime; Photophysical properties; Photophysics; PLIM; Pt complexes; Reactive oxygen species; Platinum compounds
Año:2018
Volumen:10
Número:29
Página de inicio:24361
Página de fin:24369
DOI: http://dx.doi.org/10.1021/acsami.8b02709
Título revista:ACS Applied Materials and Interfaces
Título revista abreviado:ACS Appl. Mater. Interfaces
ISSN:19448244
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19448244_v10_n29_p24361_Delcanale

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Citas:

---------- APA ----------
Delcanale, P., Galstyan, A., Daniliuc, C.G., Grecco, H.E., Abbruzzetti, S., Faust, A., Viappiani, C.,..., Strassert, C.A. (2018) . Oxygen-Insensitive Aggregates of Pt(II) Complexes as Phosphorescent Labels of Proteins with Luminescence Lifetime-Based Readouts. ACS Applied Materials and Interfaces, 10(29), 24361-24369.
http://dx.doi.org/10.1021/acsami.8b02709
---------- CHICAGO ----------
Delcanale, P., Galstyan, A., Daniliuc, C.G., Grecco, H.E., Abbruzzetti, S., Faust, A., et al. "Oxygen-Insensitive Aggregates of Pt(II) Complexes as Phosphorescent Labels of Proteins with Luminescence Lifetime-Based Readouts" . ACS Applied Materials and Interfaces 10, no. 29 (2018) : 24361-24369.
http://dx.doi.org/10.1021/acsami.8b02709
---------- MLA ----------
Delcanale, P., Galstyan, A., Daniliuc, C.G., Grecco, H.E., Abbruzzetti, S., Faust, A., et al. "Oxygen-Insensitive Aggregates of Pt(II) Complexes as Phosphorescent Labels of Proteins with Luminescence Lifetime-Based Readouts" . ACS Applied Materials and Interfaces, vol. 10, no. 29, 2018, pp. 24361-24369.
http://dx.doi.org/10.1021/acsami.8b02709
---------- VANCOUVER ----------
Delcanale, P., Galstyan, A., Daniliuc, C.G., Grecco, H.E., Abbruzzetti, S., Faust, A., et al. Oxygen-Insensitive Aggregates of Pt(II) Complexes as Phosphorescent Labels of Proteins with Luminescence Lifetime-Based Readouts. ACS Appl. Mater. Interfaces. 2018;10(29):24361-24369.
http://dx.doi.org/10.1021/acsami.8b02709