Abstract:
The importance and widespread incidence of Fusarium poae as a natural contaminant of wheat in different climatic areas warrants investigation into the genetic diversity and toxin profile of a northern Italy population. Eighty-one strains of F. poae isolated from durum wheat kernels, identified by species-specific polymerase chain reaction and translation elongation factor-1α gene sequence analysis, were genetically characterized by the amplified fragment length polymorphism (AFLP) technique and analysed by high-pressure liquid chromatography for their ability to produce the beauvericin (BEA) and trichothecene mycotoxins. A high level of variability was observed by using AFLP analyses, with the lowest level of genetic similarity among the strains being approximately 61%. Most of the strains, 95%, produced BEA at <2655 μg g-1; 88% produced the trichothecene nivalenol at <865 μgg-1 and 76% produced the trichothecene fusarenon-X at <167 μg g-1. These data show that F. poae can produce high amounts of BEA together with trichothecenes, and can represent a high potential mycotoxin risk in Italy for wheat colonized by this species. © 2010 Taylor & Francis.
Registro:
Documento: |
Artículo
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Título: | Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy |
Autor: | Somma, S.; Alvarez, C.; Ricci, V.; Ferracane, L.; Ritieni, A.; Logrieco, A.; Moretti, A. |
Filiación: | Institute of Sciences of Food Production, Research National Council (ISPA-CNR), Via Amendola 122/O, Bari I-70126, Italy Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, UBA, Ciudad Universitaria, C1428 EHA, Buenos Aires, Argentina Department of Food Sciences, University of Naples 'Federico II', Portici I-80055, Italy
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Palabras clave: | Fungi; Fusarium; Liquid chromatography/mass spectrometry (LC/MS); Mycology; Mycotoxins; Plants; Polymerase chain reaction (PCR); Trichothecenes; beauvericin; elongation factor 1alpha; fusarenon X; trichothecene; article; controlled study; food contamination; fungal colonization; fungal contamination; fungal gene; fungal genetics; fungal plant disease; fungal strain; fungus isolation; Fusarium; Fusarium poae; gene sequence; genetic variability; Italy; nonhuman; priority journal; seed kernel; wheat; Amplified Fragment Length Polymorphism Analysis; Cereals; Chromatography, High Pressure Liquid; Depsipeptides; Food Contamination; Fungal Proteins; Fusarium; Genes, Fungal; Genetic Variation; Italy; Limit of Detection; Mycological Typing Techniques; Mycotoxins; Peptide Elongation Factor 1; Phylogeny; Plant Diseases; Polymerase Chain Reaction; Species Specificity; Tandem Mass Spectrometry; Transcriptional Elongation Factors; Trichothecenes; Triticum; Fungi; Fusarium; Fusarium poae; Triticum aestivum; Triticum turgidum subsp. durum |
Año: | 2010
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Volumen: | 27
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Número: | 5
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Página de inicio: | 729
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Página de fin: | 737
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DOI: |
http://dx.doi.org/10.1080/19440040903571788 |
Título revista: | Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment
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Título revista abreviado: | Food Addit. Contam. Part A Chem. Anal. Control Exposure Risk Assess.
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ISSN: | 19440049
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CAS: | beauvericin, 26048-05-5; fusarenon X, 23255-69-8; trichothecene, 51724-48-2; Depsipeptides; Fungal Proteins; Mycotoxins; Peptide Elongation Factor 1; Transcriptional Elongation Factors; Trichothecenes; beauvericin, 26048-05-5
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19440049_v27_n5_p729_Somma |
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Citas:
---------- APA ----------
Somma, S., Alvarez, C., Ricci, V., Ferracane, L., Ritieni, A., Logrieco, A. & Moretti, A.
(2010)
. Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy. Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 27(5), 729-737.
http://dx.doi.org/10.1080/19440040903571788---------- CHICAGO ----------
Somma, S., Alvarez, C., Ricci, V., Ferracane, L., Ritieni, A., Logrieco, A., et al.
"Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy"
. Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment 27, no. 5
(2010) : 729-737.
http://dx.doi.org/10.1080/19440040903571788---------- MLA ----------
Somma, S., Alvarez, C., Ricci, V., Ferracane, L., Ritieni, A., Logrieco, A., et al.
"Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy"
. Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, vol. 27, no. 5, 2010, pp. 729-737.
http://dx.doi.org/10.1080/19440040903571788---------- VANCOUVER ----------
Somma, S., Alvarez, C., Ricci, V., Ferracane, L., Ritieni, A., Logrieco, A., et al. Trichothecene and beauvericin mycotoxin production and genetic variability in Fusarium poae isolated from wheat kernels from northern Italy. Food Addit. Contam. Part A Chem. Anal. Control Exposure Risk Assess. 2010;27(5):729-737.
http://dx.doi.org/10.1080/19440040903571788