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Fernández-Alvarez, A.J.; Pascual, M.L.; Boccaccio, G.L.; Thomas, M.G. "Smaug variants in neural and non-neuronal cells" (2016) Communicative and Integrative Biology. 9(2):1-8
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Abstract:

Mammalian Smaug1/Samd4a is an mRNA regulator involved in synapse plasticity and additional non-neuronal functions. Here we analyzed the expression of Smaug1/Samd4a variants and Smaug2/Samd4b in primary hippocampal neurons and non-neuronal cell lines. We found that multiple Smaug proteins are present in several mammalian cell lines, including a canonical full length Smaug1, a Smaug1 variant that lacks the third exon, termed DEIII, and Smaug2, the product of a highly homologous gene. These three major isoforms are expressed differentially along neuron development and form cytosolic bodies when transfected in cell lines. By using luciferase reporters, we found that the DEIII isoform, which lacks 10 amino acids in the sterile α motif involved in RNA binding, shows a RNA-binding capacity and repressor activity comparable to that of the full length Smaug1. These observations are an important groundwork for molecular studies of the Smaug posttranscriptional pathway, which is relevant to neuron development, mitochondrial function and muscle physiology in health and disease. © 2016 Ana Julia Fernández-Alvarez, Malena Lucía Pascual, Graciela Lidia Boccaccio, and María Gabriela Thomas.

Registro:

Documento: Artículo
Título:Smaug variants in neural and non-neuronal cells
Autor:Fernández-Alvarez, A.J.; Pascual, M.L.; Boccaccio, G.L.; Thomas, M.G.
Filiación:Fundación Instituto LeloirBuenos Aires, Argentina
Instituto de Investigaciones Bioquímicas Buenos Aires-Consejo Nacional de Investigaciones Científicas y TecnológicasBuenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, University of Buenos AiresBuenos Aires, Argentina
Palabras clave:mRNA silencing; Muscular dystrophy; Processing bodies; Stress granules; Synaptogenesis; Translation
Año:2016
Volumen:9
Número:2
Página de inicio:1
Página de fin:8
DOI: http://dx.doi.org/10.1080/19420889.2016.1139252
Título revista:Communicative and Integrative Biology
Título revista abreviado:Commun. Integr. Biol.
ISSN:19420889
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19420889_v9_n2_p1_FernandezAlvarez

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Citas:

---------- APA ----------
Fernández-Alvarez, A.J., Pascual, M.L., Boccaccio, G.L. & Thomas, M.G. (2016) . Smaug variants in neural and non-neuronal cells. Communicative and Integrative Biology, 9(2), 1-8.
http://dx.doi.org/10.1080/19420889.2016.1139252
---------- CHICAGO ----------
Fernández-Alvarez, A.J., Pascual, M.L., Boccaccio, G.L., Thomas, M.G. "Smaug variants in neural and non-neuronal cells" . Communicative and Integrative Biology 9, no. 2 (2016) : 1-8.
http://dx.doi.org/10.1080/19420889.2016.1139252
---------- MLA ----------
Fernández-Alvarez, A.J., Pascual, M.L., Boccaccio, G.L., Thomas, M.G. "Smaug variants in neural and non-neuronal cells" . Communicative and Integrative Biology, vol. 9, no. 2, 2016, pp. 1-8.
http://dx.doi.org/10.1080/19420889.2016.1139252
---------- VANCOUVER ----------
Fernández-Alvarez, A.J., Pascual, M.L., Boccaccio, G.L., Thomas, M.G. Smaug variants in neural and non-neuronal cells. Commun. Integr. Biol. 2016;9(2):1-8.
http://dx.doi.org/10.1080/19420889.2016.1139252