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Abstract:

The internal transcribed spacer region (ITS1 and ITS2) of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8S region from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some species of this genus were postulated to be related by the number of chromosomes. Sequence homologies in the ITS1, 5.8S and ITS2 among species are in good agreement with previous relationships established on the basis of chromosome numbers. We also indemnified a highly conserved sequence of six bp in the ITS1, reported to be present in a wide range of flowering plants, but not in the Oxalidaceae family to which the genus Oxalis belongs to. © 1996 Informa UK Ltd All rights reserved.

Registro:

Documento: Artículo
Título:Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance
Autor:Tosto, D.S.; Hopp, H.E.
Filiación:Departamento de Ciencias Biolcjgicas, Universidad de Buenos Aires, Ciudad Universitaria, CP7428, Buenos Aires, Argentina
Argentina and Lnstituto de Biotecnologia, CICV, INTA Castelar, CC77 Morcjn (1708), Argentina
Palabras clave:Andean crops; ITS; Oxalis; rDNA; plant DNA; ribosome DNA; article; genetic transcription; genetics; molecular genetics; nucleotide sequence; plant; sequence homology; Base Sequence; DNA, Plant; DNA, Ribosomal; Molecular Sequence Data; Plants; Sequence Homology, Nucleic Acid; Transcription, Genetic; Oxalis; Oxalis tuberosa; Tuberosa
Año:1996
Volumen:6
Número:6
Página de inicio:361
Página de fin:364
DOI: http://dx.doi.org/10.3109/10425179609047576
Título revista:Mitochondrial DNA
Título revista abreviado:Mitochondrial DNA
ISSN:19401736
CAS:DNA, Plant; DNA, Ribosomal
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19401736_v6_n6_p361_Tosto

Referencias:

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Citas:

---------- APA ----------
Tosto, D.S. & Hopp, H.E. (1996) . Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance. Mitochondrial DNA, 6(6), 361-364.
http://dx.doi.org/10.3109/10425179609047576
---------- CHICAGO ----------
Tosto, D.S., Hopp, H.E. "Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance" . Mitochondrial DNA 6, no. 6 (1996) : 361-364.
http://dx.doi.org/10.3109/10425179609047576
---------- MLA ----------
Tosto, D.S., Hopp, H.E. "Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance" . Mitochondrial DNA, vol. 6, no. 6, 1996, pp. 361-364.
http://dx.doi.org/10.3109/10425179609047576
---------- VANCOUVER ----------
Tosto, D.S., Hopp, H.E. Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance. Mitochondrial DNA. 1996;6(6):361-364.
http://dx.doi.org/10.3109/10425179609047576