Artículo

Urbano, F.J.; Luster, B.R.; D’onofrio, S.; Mahaffey, S.; Garcia-Rill, E. "Recording gamma band oscillations in pedunculopontine nucleus neurons" (2016) Journal of Visualized Experiments. 2016(115)
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Abstract:

Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the centrolateral/ parafascicular; Cl/Pf nucleus). The activation of either the PPN or Cl/Pf nuclei in vivo has been described to induce the arousal of the animal and an increment in gamma band activity in the cortical electroencephalogram (EEG). The cellular mechanisms for the generation of gamma band oscillations in Reticular Activating System (RAS) neurons are the same as those found to generate gamma band oscillations in other brains nuclei. During current-clamp recordings of PPN neurons (from parasagittal slices from 9 - 25 day-old rats), the use of depolarizing square steps rapidly activated voltage-dependent potassium channels that prevented PPN neurons from being depolarized beyond -25 mV. Injecting 1 - 2 sec long depolarizing current ramps gradually depolarized PPN membrane potential resting values towards 0 mV. However, injecting depolarizing square pulses generated gamma-band oscillations of membrane potential that showed to be smaller in amplitude compared to the oscillations generated by ramps. All experiments were performed in the presence of voltage-gated sodium channels and fast synaptic receptors blockers. It has been shown that the activation of high-threshold voltage-dependent calcium channels underlie gamma-band oscillatory activity in PPN neurons. Specific methodological and pharmacological interventions are described here, providing the necessary tools to induce and sustain PPN subthreshold gamma band oscillation in vitro. © 2016 Journal of Visualized Experiments.

Registro:

Documento: Artículo
Título:Recording gamma band oscillations in pedunculopontine nucleus neurons
Autor:Urbano, F.J.; Luster, B.R.; D’onofrio, S.; Mahaffey, S.; Garcia-Rill, E.
Filiación:IFIBYNE-CONICET, University of Buenos Aires, Argentina
Center for Translational Neuroscience, University of Arkansas for Medical Sciences, United States
Palabras clave:Arousal; Ca2+channels; Current ramps; Gamma oscillations; Issue 115; N-Type; Neuroscience; P/Q-type; calcium channel; animal; membrane potential; nerve cell; pedunculopontine tegmental nucleus; rat; Sprague Dawley rat; Animals; Calcium Channels; Membrane Potentials; Neurons; Pedunculopontine Tegmental Nucleus; Rats; Rats, Sprague-Dawley
Año:2016
Volumen:2016
Número:115
DOI: http://dx.doi.org/10.3791/54685
Título revista:Journal of Visualized Experiments
Título revista abreviado:J. Visualized Exp.
ISSN:1940087X
CAS:Calcium Channels
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1940087X_v2016_n115_p_Urbano

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Citas:

---------- APA ----------
Urbano, F.J., Luster, B.R., D’onofrio, S., Mahaffey, S. & Garcia-Rill, E. (2016) . Recording gamma band oscillations in pedunculopontine nucleus neurons. Journal of Visualized Experiments, 2016(115).
http://dx.doi.org/10.3791/54685
---------- CHICAGO ----------
Urbano, F.J., Luster, B.R., D’onofrio, S., Mahaffey, S., Garcia-Rill, E. "Recording gamma band oscillations in pedunculopontine nucleus neurons" . Journal of Visualized Experiments 2016, no. 115 (2016).
http://dx.doi.org/10.3791/54685
---------- MLA ----------
Urbano, F.J., Luster, B.R., D’onofrio, S., Mahaffey, S., Garcia-Rill, E. "Recording gamma band oscillations in pedunculopontine nucleus neurons" . Journal of Visualized Experiments, vol. 2016, no. 115, 2016.
http://dx.doi.org/10.3791/54685
---------- VANCOUVER ----------
Urbano, F.J., Luster, B.R., D’onofrio, S., Mahaffey, S., Garcia-Rill, E. Recording gamma band oscillations in pedunculopontine nucleus neurons. J. Visualized Exp. 2016;2016(115).
http://dx.doi.org/10.3791/54685