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Abstract:

The mammalian sperm nucleus contains an unusually condensed chromatin, due to replacement of the majority of histones by protamines. However, soon after the spermatozoon penetrates the ooplasm at fertilization, decondensation of this densely packed chromatin must occur to allow formation of the male pronucleus and syngamy. Decondensation is accomplished by protamine disulfide bond reduction by oocyte glutathione and replacement of protamines by oocyte histones with the aid of an acceptor molecule. Previous results from our laboratory have demonstrated that heparan sulfate (HS) present in the ooplasm functions as protamine acceptor during human sperm decondensation in vivo. In the present paper, we analyze the role of heparin, structural analogue of HS, and dermatan sulfate (DS) in murine sperm chromatin decondensation in vitro, including the possibility of a synergistic effect between both glycosaminoglycans. Decondensation was assessed under phase contrast microscopy following incubation of murine spermatozoa with glutathione and either heparin, DS, or a combination of both. Ultrastructural changes taking place during decondensation were analyzed by transmission electron microscopy. Both glycosaminoglycans were able to promote the decondensation of murine spermatozoa in vitro but the decondensing ability of heparin was significantly higher. Use of both glycosaminoglycans together revealed the existence of a synergistic effect. Transmission electron microscopy analysis of decondensing spermatozoa supported these findings. Synergism between heparin and DS was observed both in capacitated and non-capacitated spermatozoa but decondensation kinetics was faster in the former. The results obtained indicate a new potential role for dermatan sulfate in murine sperm decondensation at fertilization and provide evidence of differences in the degree of chromatin condensation throughout the murine sperm nucleus. © 2013 Informa Healthcare USA, Inc.

Registro:

Documento: Artículo
Título:Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation
Autor:Sanchez, M.C.; Alvarez Sedo, C.; Julianelli, V.L.; Romanato, M.; Calvo, L.; Calvo, J.C.; Fontana, V.A.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, National Council for Science and Technology (CONICET) Argentina, Buenos Aires, Argentina
CEGYR, Buenos Aires, Argentina
Palabras clave:Chromatin decondensation; Dermatan sulfate; Heparin; Murine spermatozoa; Synergism; dermatan sulfate; glutathione; glycosaminoglycan; heparin; animal cell; article; chromatin; chromatin decondensation'; controlled study; dose response; in vitro study; male; mouse; nonhuman; phase contrast microscopy; priority journal; spermatozoon; transmission electron microscopy; Animals; Chromatin; Dermatan Sulfate; Dose-Response Relationship, Drug; Heparin; Male; Mice; Microscopy, Electron; Spermatozoa
Año:2013
Volumen:59
Número:2
Página de inicio:82
Página de fin:90
DOI: http://dx.doi.org/10.3109/19396368.2012.756952
Título revista:Systems Biology in Reproductive Medicine
Título revista abreviado:Syst. Biology Reprod. Med.
ISSN:19396368
CAS:dermatan sulfate, 24967-94-0; glutathione, 70-18-8; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; Chromatin; Dermatan Sulfate, 24967-94-0; Heparin, 9005-49-6
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_19396368_v59_n2_p82_Sanchez.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19396368_v59_n2_p82_Sanchez

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Citas:

---------- APA ----------
Sanchez, M.C., Alvarez Sedo, C., Julianelli, V.L., Romanato, M., Calvo, L., Calvo, J.C. & Fontana, V.A. (2013) . Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation. Systems Biology in Reproductive Medicine, 59(2), 82-90.
http://dx.doi.org/10.3109/19396368.2012.756952
---------- CHICAGO ----------
Sanchez, M.C., Alvarez Sedo, C., Julianelli, V.L., Romanato, M., Calvo, L., Calvo, J.C., et al. "Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation" . Systems Biology in Reproductive Medicine 59, no. 2 (2013) : 82-90.
http://dx.doi.org/10.3109/19396368.2012.756952
---------- MLA ----------
Sanchez, M.C., Alvarez Sedo, C., Julianelli, V.L., Romanato, M., Calvo, L., Calvo, J.C., et al. "Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation" . Systems Biology in Reproductive Medicine, vol. 59, no. 2, 2013, pp. 82-90.
http://dx.doi.org/10.3109/19396368.2012.756952
---------- VANCOUVER ----------
Sanchez, M.C., Alvarez Sedo, C., Julianelli, V.L., Romanato, M., Calvo, L., Calvo, J.C., et al. Dermatan sulfate synergizes with heparin in murine sperm chromatin decondensation. Syst. Biology Reprod. Med. 2013;59(2):82-90.
http://dx.doi.org/10.3109/19396368.2012.756952