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Abstract:

A strain of the lichen mycobiont of Ramalina celastri, isolated from ascospores, was cultured axenically on two solid media containing high amounts of the carbon source: sucrose in MY10 and mannitol in BMRM. Usnic acid, the major cortical lichen metabolite, was produced by the colonies grown on MY10, with a very high yield (7.9%) in comparison with that in the lichen thallus. Mycelia grown on BMRM did not produce the lichen secondary metabolite and rendered triacylglycerides (8.5%) instead. Analysis by GC-MS of the fatty acid methyl esters revealed the presence of oleic, palmitic and stearic acids as the main triacylglyceride constituents. The present results highlight the impact of the culture conditions on the lichen mycobiont secondary metabolism and confirm that MY10 is a useful medium to obtain usnic acid from mycobionts in the laboratory.

Registro:

Documento: Artículo
Título:Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri
Autor:Fazio, A.T.; Adler, M.T.; Maier, M.S.
Filiación:PROPLAME-PRHIDEB - Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Ciudad Autónoma de Buenos Aires, Argentina
UMYMFOR - Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Axenic cultures; Lichen metabolites; Ramalina celastri; Triacylglycerides; Usnic acid; Benzofurans; Lichens; Symbiosis; Triglycerides
Año:2014
Volumen:9
Número:2
Página de inicio:213
Página de fin:214
Título revista:Natural Product Communications
Título revista abreviado:Nat. Pro. Comm.
ISSN:1934578X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1934578X_v9_n2_p213_Fazio

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Citas:

---------- APA ----------
Fazio, A.T., Adler, M.T. & Maier, M.S. (2014) . Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri. Natural Product Communications, 9(2), 213-214.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1934578X_v9_n2_p213_Fazio [ ]
---------- CHICAGO ----------
Fazio, A.T., Adler, M.T., Maier, M.S. "Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri" . Natural Product Communications 9, no. 2 (2014) : 213-214.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1934578X_v9_n2_p213_Fazio [ ]
---------- MLA ----------
Fazio, A.T., Adler, M.T., Maier, M.S. "Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri" . Natural Product Communications, vol. 9, no. 2, 2014, pp. 213-214.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1934578X_v9_n2_p213_Fazio [ ]
---------- VANCOUVER ----------
Fazio, A.T., Adler, M.T., Maier, M.S. Usnic acid and triacylglycerides production by the cultured lichen mycobiont of Ramalina celastri. Nat. Pro. Comm. 2014;9(2):213-214.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1934578X_v9_n2_p213_Fazio [ ]