Artículo
Abstract:
This unit describes a method for quantifying various cellular parameters (e.g., volume, total and subcellular fluorescence localization) from sets of microscope images of individual cells. It includes procedures for tracking cells over time. One purposefully defocused transmission image (sometimes referred to as bright-field or BF) is acquired to locate each cell. Fluorescent images (one for each of the color channels to be analyzed) are then acquired by conventional wide-field epifluorescence or confocal microscopy. This method uses the image processing capabilities of Cell-ID (Gordon et al., 2007) and data analysis by the statistical programming framework R (R-Development-Team, 2008), which we have supplemented with a package tailored to analyze Cell-ID output. Both programs are open-source software packages. Copyright 2008 by John Wiley & Sons, Inc.
Registro:
| Documento: | Artículo |
| Título: | Using Cell-ID 1.4 with R for microscope-based cytometry. |
| Autor: | Chernomoretz, A.; Bush, A.; Yu, R.; Gordon, A.; Colman-Lerner, A. |
| Filiación: | Physics Department, FCEN, University of Buenos Aires and CONICET, Buenos Aires, Argentina |
| Palabras clave: | animal; article; cell; computer program; confocal microscopy; cytology; fluorescence microscopy; human; image processing; instrumentation; methodology; statistical analysis; yeast; Animals; Cells; Data Interpretation, Statistical; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Microscopy, Fluorescence; Software; Yeasts |
| Año: | 2008 |
| Volumen: | Chapter 14 |
| Título revista: | Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] |
| Título revista abreviado: | Curr Protoc Mol Biol |
| ISSN: | 19343647 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19343647_vChapter14_n_p_Chernomoretz |
Citas:
---------- APA ----------
Chernomoretz, A., Bush, A., Yu, R., Gordon, A. & Colman-Lerner, A. (2008) . Using Cell-ID 1.4 with R for microscope-based cytometry. Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], Chapter 14.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19343647_vChapter14_n_p_Chernomoretz [ ]
---------- CHICAGO ----------
Chernomoretz, A., Bush, A., Yu, R., Gordon, A., Colman-Lerner, A. "Using Cell-ID 1.4 with R for microscope-based cytometry." Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] Chapter 14 (2008).Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19343647_vChapter14_n_p_Chernomoretz [ ]
---------- MLA ----------
Chernomoretz, A., Bush, A., Yu, R., Gordon, A., Colman-Lerner, A. "Using Cell-ID 1.4 with R for microscope-based cytometry." Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.], vol. Chapter 14, 2008.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19343647_vChapter14_n_p_Chernomoretz [ ]
---------- VANCOUVER ----------
Chernomoretz, A., Bush, A., Yu, R., Gordon, A., Colman-Lerner, A. Using Cell-ID 1.4 with R for microscope-based cytometry. Curr Protoc Mol Biol. 2008;Chapter 14.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19343647_vChapter14_n_p_Chernomoretz [ ]
