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Abstract:

Cell line establishment of somatic cells is a valuable resource to preserve genetic material of rare, difficult-to-find, endangered and giant species like Jaguar (Panthera onca), the largest South American felid. This unit focuses on the isolation and culture of fibroblasts from Jaguar skin and muscle biopsies, and ear cartilage dissection immediately after death to preserve one of the several endangered species in this biome. These culture techniques enabled us to contribute 570 samples from 45 autochthonous and endangered species, including Jaguar. The fibroblasts obtained are a part of the Genetic Bank of Buenos Aires Zoo with the 6700 samples, including tissues such as muscle, ovarian, testicular, blood, fibroblast cultures, sperm, hair, and fluids and cells from 450 individuals of 87 different species. © 2016 by John Wiley & Sons, Inc.

Registro:

Documento: Artículo
Título:Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species
Autor:Mestre-Citrinovitz, A.C.; Sestelo, A.J.; Ceballos, M.B.; Barañao, J.L.; Saragüeta, P.
Filiación:Instituto de Biología y Medicina Experimental, IByME-CONICET, Buenos Aires, Argentina
Zoológico de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Palabras clave:Biobank; Cell culture; Panthera onca; animal cell; animal tissue; Article; biobank; biome; blood storage; Brazil; cell culture; cell culture technique; cell isolation; cryopreservation; ear cartilage; endangered species; fibroblast; genetic resource; hair cell; jaguar; muscle biopsy; muscle tissue; nonhuman; ovary tissue; priority journal; skin fibroblast; somatic cell; South American; sperm preservation; testis tissue; wildlife conservation; animal; cartilage; cell separation; cytology; fibroblast; metabolism; muscle; Panthera; procedures; skin; Animals; Cartilage; Cell Culture Techniques; Cell Separation; Cells, Cultured; Cryopreservation; Endangered Species; Fibroblasts; Muscles; Panthera; Skin
Año:2016
Volumen:2016
DOI: http://dx.doi.org/10.1002/cpmb.25
Título revista:Current Protocols in Molecular Biology
Título revista abreviado:Curr. Protoc. Mol. Biol.
ISSN:19343639
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19343639_v2016_n_p_MestreCitrinovitz

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Citas:

---------- APA ----------
Mestre-Citrinovitz, A.C., Sestelo, A.J., Ceballos, M.B., Barañao, J.L. & Saragüeta, P. (2016) . Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species. Current Protocols in Molecular Biology, 2016.
http://dx.doi.org/10.1002/cpmb.25
---------- CHICAGO ----------
Mestre-Citrinovitz, A.C., Sestelo, A.J., Ceballos, M.B., Barañao, J.L., Saragüeta, P. "Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species" . Current Protocols in Molecular Biology 2016 (2016).
http://dx.doi.org/10.1002/cpmb.25
---------- MLA ----------
Mestre-Citrinovitz, A.C., Sestelo, A.J., Ceballos, M.B., Barañao, J.L., Saragüeta, P. "Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species" . Current Protocols in Molecular Biology, vol. 2016, 2016.
http://dx.doi.org/10.1002/cpmb.25
---------- VANCOUVER ----------
Mestre-Citrinovitz, A.C., Sestelo, A.J., Ceballos, M.B., Barañao, J.L., Saragüeta, P. Isolation of primary fibroblast culture from wildlife: The Panthera onca case to preserve a South American endangered species. Curr. Protoc. Mol. Biol. 2016;2016.
http://dx.doi.org/10.1002/cpmb.25