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Abstract:

The aim of this study was to identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captivity. The localization and distribution of growth hormone (GH), prolactin (PRL), somatolactin (SL), β-luteinizing hormone (β-LH), and β-follicle stimulating hormone (β-FSH) immunoreactive (-ir) cells in the adenohypophysis was studied by immunohistochemical and Western blot methods. In addition, cellular morphometric analyses and semi-quantification of ir-cells optical density (OD) during the annual reproductive cycle and after artificial induced spawning (AIS) were performed. Results showed that the distribution and general localization of pituitary cell types were similar to that of other teleost species. However, the morphometrical study of adenohypophysial cells showed differences along the reproductive cycle and following AIS. In general, females at the vitellogenic stage presented greater OD values for GH, PRL and SL than at other maturation stages (previtellogenic and regression stages), probably indicating an increased cellular activity during this stage. Conversely, β-LH OD did not vary during the annual reproductive cycle. After AIS, β-LH, SL and GH ir-cells showed an increase in OD values suggesting a possible involvement on oocyte final maturation, ovulation and spawning or a feedback control on the brain-pituitary-gonads axis. Reproductive dysfunction in S. parahybae females in captivity may be due to alteration of the synthesis pathways of β-LH. In addition, GH family of hormones could modulate associated mechanisms that influence the reproductive status in this species. © 2015 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity
Autor:Honji, R.M.; Caneppele, D.; Pandolfi, M.; Lo Nostro, F.L.; Moreira, R.G.
Filiación:Departamento De Fisiologia, Instituto De Biociências, Universidade De São Paulo, Rua Do Matão, Travessa 14, 321, São Paulo, SP, 05508-090, Brazil
Departamento De Biodiversidad Y Biología Experimental, Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires and IBBEA, CONICET-UBA. Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina
Companhia Energética De São Paulo (CESP), Unidade De Hidrobiologia E Aquicultura, Rodovia Dos Tamoios, Km 3812260-000, Brazil
Palabras clave:Follicle stimulating hormone; Growth hormone; Luteinizing hormone; Prolactin hormone; Reproductive dysfunction; Somatolactin hormone; follitropin beta subunit; gonadotropin; growth hormone; luteinizing hormone beta subunit; prolactin; somatolactin; unclassified drug; fish protein; gonadotropin; growth hormone; adenohypophysis; animal tissue; Article; cell structure; cellular distribution; controlled study; feedback system; female; genital system disease; hypophysis; hypophysis cell; immunohistochemistry; nonhuman; oocyte development; oocyte maturation; optical density; ovary cycle; ovary development; ovulation; priority journal; reproductive success; Siluriformes; spawning; teleost; Western blotting; animal; catfish; classification; cytology; embryology; endangered species; metabolism; nonmammalian embryo; oocyte; reproduction; vitellogenesis; Pimelodidae; Siluriformes; Teleostei; Animals; Blotting, Western; Catfishes; Embryo, Nonmammalian; Endangered Species; Female; Fish Proteins; Gonadotropins; Growth Hormone; Immunohistochemistry; Oocytes; Pituitary Gland; Reproduction; Vitellogenesis
Año:2015
Volumen:298
Número:9
Página de inicio:1644
Página de fin:1658
DOI: http://dx.doi.org/10.1002/ar.23174
Título revista:Anatomical Record
Título revista abreviado:Anat. Rec.
ISSN:19328486
CAS:gonadotropin, 63231-54-9; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; prolactin, 12585-34-1, 50647-00-2, 9002-62-4; Fish Proteins; Gonadotropins; Growth Hormone
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19328486_v298_n9_p1644_Honji

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Citas:

---------- APA ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L. & Moreira, R.G. (2015) . Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity. Anatomical Record, 298(9), 1644-1658.
http://dx.doi.org/10.1002/ar.23174
---------- CHICAGO ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L., Moreira, R.G. "Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity" . Anatomical Record 298, no. 9 (2015) : 1644-1658.
http://dx.doi.org/10.1002/ar.23174
---------- MLA ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L., Moreira, R.G. "Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity" . Anatomical Record, vol. 298, no. 9, 2015, pp. 1644-1658.
http://dx.doi.org/10.1002/ar.23174
---------- VANCOUVER ----------
Honji, R.M., Caneppele, D., Pandolfi, M., Lo Nostro, F.L., Moreira, R.G. Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity. Anat. Rec. 2015;298(9):1644-1658.
http://dx.doi.org/10.1002/ar.23174