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Cusido, J.; Ragab, S.S.; Thapaliya, E.R.; Swaminathan, S.; Garcia-Amorós, J.; Roberti, M.J.; Araoz, B.; Mazza, M.M.A.; Yamazaki, S.; Scott, A.M.; Raymo, F.M.; Bossi, M.L. "A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging" (2016) Journal of Physical Chemistry C. 120(23):12860-12870
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Abstract:

A coumarin fluorophore and an oxazine photochrome can be integrated within the same molecular skeleton and connected covalently to a secondary antibody. Illumination of the antibody-dyad conjugate at an appropriate activation wavelength opens the oxazine ring reversibly and shifts bathochromically the ground-state absorption of the coumarin component. Selective excitation of the photochemical product then produces significant fluorescence and allows the detection of activated bioconjugates at the single-molecule level. Such fluorescence activation events can be exploited to resolve temporally individual emitters and reconstruct images of immunolabeled cells with subdiffraction resolution. Relying on a similar conjugation protocol, a model compound, incorporating the same chromophore of the photochemical product, can also be connected covalently to a secondary antibody. Stimulated emission can be exploited to deplete the excited state of the bioconjugated chromophores and switch their fluorescence off. These operating principles for fluorescence switching also permit the imaging of immunolabeled cells with subdiffraction resolution. Thus, these photoswitchable molecules, in combination with the labeling ability of antibodies, can evolve into valuable probes for bioimaging with superresolution. © 2016 American Chemical Society.

Registro:

Documento: Artículo
Título:A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging
Autor:Cusido, J.; Ragab, S.S.; Thapaliya, E.R.; Swaminathan, S.; Garcia-Amorós, J.; Roberti, M.J.; Araoz, B.; Mazza, M.M.A.; Yamazaki, S.; Scott, A.M.; Raymo, F.M.; Bossi, M.L.
Filiación:Laboratory for Molecular Photonics, Department of Chemistry, University of Miami, 1301 Memorial Drive, Coral Gables, FL 33146-0431, United States
Department of Photochemistry, Chemical Industries Research Division, National Research Centre (NRC), El behouth Street, Dokki, Giza, 12622, Egypt
INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires, 1428, Argentina
Palabras clave:Biomolecules; Chemical activation; Chromophores; Excited states; Fluorescence; Ground state; Molecules; Nitrogen compounds; Optical resolving power; Fluorescence switching; Ground state absorption; Molecular skeleton; Operating principles; Secondary antibodies; Selective excitations; Single molecule level; Super resolution imaging; Antibodies
Año:2016
Volumen:120
Número:23
Página de inicio:12860
Página de fin:12870
DOI: http://dx.doi.org/10.1021/acs.jpcc.6b03135
Título revista:Journal of Physical Chemistry C
Título revista abreviado:J. Phys. Chem. C
ISSN:19327447
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19327447_v120_n23_p12860_Cusido

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Citas:

---------- APA ----------
Cusido, J., Ragab, S.S., Thapaliya, E.R., Swaminathan, S., Garcia-Amorós, J., Roberti, M.J., Araoz, B.,..., Bossi, M.L. (2016) . A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging. Journal of Physical Chemistry C, 120(23), 12860-12870.
http://dx.doi.org/10.1021/acs.jpcc.6b03135
---------- CHICAGO ----------
Cusido, J., Ragab, S.S., Thapaliya, E.R., Swaminathan, S., Garcia-Amorós, J., Roberti, M.J., et al. "A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging" . Journal of Physical Chemistry C 120, no. 23 (2016) : 12860-12870.
http://dx.doi.org/10.1021/acs.jpcc.6b03135
---------- MLA ----------
Cusido, J., Ragab, S.S., Thapaliya, E.R., Swaminathan, S., Garcia-Amorós, J., Roberti, M.J., et al. "A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging" . Journal of Physical Chemistry C, vol. 120, no. 23, 2016, pp. 12860-12870.
http://dx.doi.org/10.1021/acs.jpcc.6b03135
---------- VANCOUVER ----------
Cusido, J., Ragab, S.S., Thapaliya, E.R., Swaminathan, S., Garcia-Amorós, J., Roberti, M.J., et al. A Photochromic Bioconjugate with Photoactivatable Fluorescence for Superresolution Imaging. J. Phys. Chem. C. 2016;120(23):12860-12870.
http://dx.doi.org/10.1021/acs.jpcc.6b03135