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Toro, A.R.; Maymó, J.L.; Ibarbalz, F.M.; Pérez, A.; Maskin, B.; Faletti, A.G.; Margalet, V.S.; Varone, C.L. "Leptin is an anti-apoptotic effector in placental cells involving p53 downregulation" (2014) PLoS ONE. 9(6)
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Abstract:

Leptin, a peripheral signal synthetized by the adipocyte to regulate energy metabolism, can also be produced by placenta, where it may work as an autocrine hormone. We have previously demonstrated that leptin promotes proliferation and survival of trophoblastic cells. In the present work, we aimed to study the molecular mechanisms that mediate the survival effect of leptin in placenta. We used the human placenta choriocarcinoma BeWo and first trimester Swan-71 cell lines, as well as human placental explants. We tested the late phase of apoptosis, triggered by serum deprivation, by studying the activation of Caspase-3 and DNA fragmentation. Recombinant human leptin added to BeWo cell line and human placental explants, showed a decrease on Caspase-3 activation. These effects were dose dependent. Maximal effect was achieved at 250 ng leptin/ml. Moreover, inhibition of endogenous leptin expression with 2 mM of an antisense oligonucleotide, reversed Caspase-3 diminution. We also found that the cleavage of Poly [ADP-ribose] polymerase-1 (PARP-1) was diminished in the presence of leptin. We analyzed the presence of low DNA fragments, products from apoptotic DNA cleavage. Placental explants cultivated in the absence of serum in the culture media increased the apoptotic cleavage of DNA and this effect was prevented by the addition of 100 ng leptin/ml. Taken together these results reinforce the survival effect exerted by leptin on placental cells. To improve the understanding of leptin mechanism in regulating the process of apoptosis we determined the expression of different intermediaries in the apoptosis cascade. We found that under serum deprivation conditions, leptin increased the anti-apoptotic BCL-2 protein expression, while downregulated the pro-apoptotic BAX and BID proteins expression in Swan-71 cells and placental explants. In both models leptin augmented BCL-2/BAX ratio. Moreover we have demonstrated that p53, one of the key cell cycle-signaling proteins, is downregulated in the presence of leptin under serum deprivation. On the other hand, we determined that leptin reduced the phosphorylation of Ser-46 p53 that plays a pivotal role for apoptotic signaling by p53. Our data suggest that the observed anti-apoptotic effect of leptin in placenta is in part mediated by the p53 pathway. In conclusion, we provide evidence that demonstrates that leptin is a trophic factor for trophoblastic cells. © 2014 Toro et al.

Registro:

Documento: Artículo
Título:Leptin is an anti-apoptotic effector in placental cells involving p53 downregulation
Autor:Toro, A.R.; Maymó, J.L.; Ibarbalz, F.M.; Pérez, A.; Maskin, B.; Faletti, A.G.; Margalet, V.S.; Varone, C.L.
Filiación:Departamento de Química Biológica, Instituto de Química Biológica de la Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Bioquímica Médica Y Biología Molecular, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Sevilla, Spain
Hospital Nacional Profesor Alejandro Posadas, Buenos Aires, Argentina
Centro de Estudios Farmacológicos Y Botánicos, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:antisense oligonucleotide; caspase 3; leptin; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1; protein Bax; protein bcl 2; protein Bid; protein p53; serine; leptin; phosphoserine; protein Bax; protein Bid; protein p53; apoptosis; article; carcinoma cell; cell cycle; cell survival; choriocarcinoma; controlled study; DNA cleavage; DNA fragmentation; down regulation; enzyme activation; explant; human; human cell; human tissue; protein cleavage; protein expression; protein function; protein phosphorylation; signal transduction; trophoblast; cytology; down regulation; female; metabolism; phosphorylation; placenta; pregnancy; trophoblast; tumor cell line; Apoptosis; bcl-2-Associated X Protein; BH3 Interacting Domain Death Agonist Protein; Cell Line, Tumor; Down-Regulation; Female; Humans; Leptin; Phosphorylation; Phosphoserine; Placenta; Pregnancy; Trophoblasts; Tumor Suppressor Protein p53
Año:2014
Volumen:9
Número:6
DOI: http://dx.doi.org/10.1371/journal.pone.0099187
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:caspase 3, 169592-56-7; protein bcl 2, 219306-68-0; protein Bid, 260235-79-8; serine, 56-45-1, 6898-95-9; phosphoserine, 407-41-0; bcl-2-Associated X Protein; BH3 Interacting Domain Death Agonist Protein; Leptin; Phosphoserine; Tumor Suppressor Protein p53
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v9_n6_p_Toro

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Citas:

---------- APA ----------
Toro, A.R., Maymó, J.L., Ibarbalz, F.M., Pérez, A., Maskin, B., Faletti, A.G., Margalet, V.S.,..., Varone, C.L. (2014) . Leptin is an anti-apoptotic effector in placental cells involving p53 downregulation. PLoS ONE, 9(6).
http://dx.doi.org/10.1371/journal.pone.0099187
---------- CHICAGO ----------
Toro, A.R., Maymó, J.L., Ibarbalz, F.M., Pérez, A., Maskin, B., Faletti, A.G., et al. "Leptin is an anti-apoptotic effector in placental cells involving p53 downregulation" . PLoS ONE 9, no. 6 (2014).
http://dx.doi.org/10.1371/journal.pone.0099187
---------- MLA ----------
Toro, A.R., Maymó, J.L., Ibarbalz, F.M., Pérez, A., Maskin, B., Faletti, A.G., et al. "Leptin is an anti-apoptotic effector in placental cells involving p53 downregulation" . PLoS ONE, vol. 9, no. 6, 2014.
http://dx.doi.org/10.1371/journal.pone.0099187
---------- VANCOUVER ----------
Toro, A.R., Maymó, J.L., Ibarbalz, F.M., Pérez, A., Maskin, B., Faletti, A.G., et al. Leptin is an anti-apoptotic effector in placental cells involving p53 downregulation. PLoS ONE. 2014;9(6).
http://dx.doi.org/10.1371/journal.pone.0099187