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Abstract:

It is generally accepted that the immediately releasable pool is a group of readily releasable vesicles that are closely associated with voltage dependent Ca2+ channels. We have previously shown that exocytosis of this pool is specifically coupled to P/Q Ca2+ current. Accordingly, in the present work we found that the Ca2+ current flowing through P/Q-type Ca2+ channels is 8 times more effective at inducing exocytosis in response to short stimuli than the current carried by L-type channels. To investigate the mechanism that underlies the coupling between the immediately releasable pool and P/Q-type channels we transiently expressed in mouse chromaffin cells peptides corresponding to the synaptic protein interaction site of Cav2.2 to competitively uncouple P/Q-type channels from the secretory vesicle release complex. This treatment reduced the efficiency of Ca2+ current to induce exocytosis to similar values as direct inhibition of P/Q-type channels via ω-agatoxin-IVA. In addition, the same treatment markedly reduced immediately releasable pool exocytosis, but did not affect the exocytosis provoked by sustained electric or high K+ stimulation. Together, our results indicate that the synaptic protein interaction site is a crucial factor for the establishment of the functional coupling between immediately releasable pool vesicles and P/Q-type Ca2+ channels. © 2013 Álvarez et al.

Registro:

Documento: Artículo
Título:The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site
Autor:Álvarez, Y.D.; Belingheri, A.V.; Perez Bay, A.E.; Javis, S.E.; Tedford, H.W.; Zamponi, G.; Marengo, F.D.
Filiación:Lab. de Fisiologia y Bio. Molecular, Inst. de Fisiologia, Bio. Molecular y Neurociencias (CONICET), Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Physiology and Pharmacology, Hotchkiss Brain Institute, University of Calgary, Calgary, Canada
Department of Ophthalmology, Dyson Vision Research Institute, Weill Medical College, Cornell University, New York, NY, United States
Palabras clave:calcium channel P type; calcium channel Q type; omega agatoxin IVA; potassium ion; animal cell; article; binding site; calcium current; cell interaction; cell vacuole; chromaffin cell; controlled study; depolarization; exocytosis; mouse; nerve cell stimulation; nonhuman; protein expression; protein interaction; sequence analysis; synapse; transient expression; Animals; Calcium; Calcium Channels, P-Type; Calcium Channels, Q-Type; Cells, Cultured; Chromaffin Cells; Exocytosis; Mice; Secretory Vesicles
Año:2013
Volumen:8
Número:1
DOI: http://dx.doi.org/10.1371/journal.pone.0054846
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CAS:potassium ion, 24203-36-9; Calcium, 7440-70-2; Calcium Channels, P-Type; Calcium Channels, Q-Type
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_19326203_v8_n1_p_Alvarez.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n1_p_Alvarez

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Citas:

---------- APA ----------
Álvarez, Y.D., Belingheri, A.V., Perez Bay, A.E., Javis, S.E., Tedford, H.W., Zamponi, G. & Marengo, F.D. (2013) . The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site. PLoS ONE, 8(1).
http://dx.doi.org/10.1371/journal.pone.0054846
---------- CHICAGO ----------
Álvarez, Y.D., Belingheri, A.V., Perez Bay, A.E., Javis, S.E., Tedford, H.W., Zamponi, G., et al. "The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site" . PLoS ONE 8, no. 1 (2013).
http://dx.doi.org/10.1371/journal.pone.0054846
---------- MLA ----------
Álvarez, Y.D., Belingheri, A.V., Perez Bay, A.E., Javis, S.E., Tedford, H.W., Zamponi, G., et al. "The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site" . PLoS ONE, vol. 8, no. 1, 2013.
http://dx.doi.org/10.1371/journal.pone.0054846
---------- VANCOUVER ----------
Álvarez, Y.D., Belingheri, A.V., Perez Bay, A.E., Javis, S.E., Tedford, H.W., Zamponi, G., et al. The Immediately Releasable Pool of Mouse Chromaffin Cell Vesicles Is Coupled to P/Q-Type Calcium Channels via the Synaptic Protein Interaction Site. PLoS ONE. 2013;8(1).
http://dx.doi.org/10.1371/journal.pone.0054846