Artículo

Losino, N.; Waisman, A.; Solari, C.; Luzzani, C.; Espinosa, D.F.; Sassone, A.; Muro, A.F.; Miriuka, S.; Sevlever, G.; Barañao, L.; Guberman, A. "EDA-containing fibronectin increases proliferation of embryonic stem cells" (2013) PLoS ONE. 8(11)
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Abstract:

Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from a bovine granulosa cell line BGC (BGC-CM), a medium that not only preserves their main features but also increases ESC's proliferation rate. The mitogenic properties of this medium were previously reported, ascribing this effect to an alternative spliced generated fibronectin isoform that contains the extra domain A (FN EDA +). Here, we investigated if the FN EDA+ isoform increased proliferation of mouse and human ES cells. We analyzed cell proliferation using conditioned media produced by different mouse embryonic fibroblast (MEF) lines genetically engineered to express FN constitutively including or excluding the EDA domain (FN EDA-), and in media supplemented with recombinant peptides containing or not the EDA. We found that the presence of EDA in the medium increased mouse and human ESC's proliferation rate. Here we showed for the first time that this FN isoform enhances ESC's proliferation. These findings suggest a possible conserved behavior for regulation of ES cells proliferation by this FN isoform and could contribute to improve their culturing conditions both for research and cell therapy. © 2013 Losino et al.

Registro:

Documento: Artículo
Título:EDA-containing fibronectin increases proliferation of embryonic stem cells
Autor:Losino, N.; Waisman, A.; Solari, C.; Luzzani, C.; Espinosa, D.F.; Sassone, A.; Muro, A.F.; Miriuka, S.; Sevlever, G.; Barañao, L.; Guberman, A.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Ciudad de Buenos Aires, Argentina
Instituto de Química Biológica - Ciencias Exactas y Naturales (IQUIBICEN), UBA/Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad de Buenos Aires, Argentina
Laboratorio de Biología del Desarrollo Celular, Fundación para la Lucha Contra las Enfermedades Neurológicas de la Infancia (FLENI), Buenos Aires, Argentina
International Centre for Genetic Engineering and Biotechnology, Trieste, Italy
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Palabras clave:culture medium; extra domain A fibronectin, human; fibronectin; animal; cell culture; cell proliferation; culture medium; cytology; drug effects; embryonic stem cell; fibroblast; genetics; human; metabolism; mouse; mutant mouse strain; pharmacology; Animals; Cell Proliferation; Cells, Cultured; Culture Media, Conditioned; Embryonic Stem Cells; Fibroblasts; Fibronectins; Humans; Mice; Mice, Mutant Strains
Año:2013
Volumen:8
Número:11
DOI: http://dx.doi.org/10.1371/journal.pone.0080681
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:fibronectin, 86088-83-7; Culture Media, Conditioned; extra domain A fibronectin, human; Fibronectins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v8_n11_p_Losino

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Citas:

---------- APA ----------
Losino, N., Waisman, A., Solari, C., Luzzani, C., Espinosa, D.F., Sassone, A., Muro, A.F.,..., Guberman, A. (2013) . EDA-containing fibronectin increases proliferation of embryonic stem cells. PLoS ONE, 8(11).
http://dx.doi.org/10.1371/journal.pone.0080681
---------- CHICAGO ----------
Losino, N., Waisman, A., Solari, C., Luzzani, C., Espinosa, D.F., Sassone, A., et al. "EDA-containing fibronectin increases proliferation of embryonic stem cells" . PLoS ONE 8, no. 11 (2013).
http://dx.doi.org/10.1371/journal.pone.0080681
---------- MLA ----------
Losino, N., Waisman, A., Solari, C., Luzzani, C., Espinosa, D.F., Sassone, A., et al. "EDA-containing fibronectin increases proliferation of embryonic stem cells" . PLoS ONE, vol. 8, no. 11, 2013.
http://dx.doi.org/10.1371/journal.pone.0080681
---------- VANCOUVER ----------
Losino, N., Waisman, A., Solari, C., Luzzani, C., Espinosa, D.F., Sassone, A., et al. EDA-containing fibronectin increases proliferation of embryonic stem cells. PLoS ONE. 2013;8(11).
http://dx.doi.org/10.1371/journal.pone.0080681