Artículo

Fuentes, F.; Zimmer, D.; Atienza, M.; Schottenfeld, J.; Penkala, I.; Bale, T.; Bence, K.K.; Arregui, C.O. "Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning" (2012) PLoS ONE. 7(7)
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Abstract:

ER-bound PTP1B is expressed in hippocampal neurons, and accumulates among neurite contacts. PTP1B dephosphorylates ß-catenin in N-cadherin complexes ensuring cell-cell adhesion. Here we show that endogenous PTP1B, as well as expressed GFP-PTP1B, are present in dendritic spines of hippocampal neurons in culture. GFP-PTP1B overexpression does not affect filopodial density or length. In contrast, impairment of PTP1B function or genetic PTP1B-deficiency leads to increased filopodia-like dendritic spines and a reduction in mushroom-like spines, while spine density is unaffected. These morphological alterations are accompanied by a disorganization of pre- and post-synapses, as judged by decreased clustering of synapsin-1 and PSD-95, and suggest a dynamic synaptic phenotype. Notably, levels of ß-catenin-Tyr-654 phosphorylation increased ~5-fold in the hippocampus of adult PTP1B-/- (KO) mice compared to wild type (WT) mice and this was accompanied by a reduction in the amount of ß-catenin associated with N-cadherin. To determine whether PTP1B-deficiency alters learning and memory, we generated mice lacking PTP1B in the hippocampus and cortex (PTP1Bfl/fl-Emx1-Cre). PTP1Bfl/fl-Emx1-Cre mice displayed improved performance in the Barnes maze (decreased time to find and enter target hole), utilized a more efficient strategy (cued), and had better recall compared to WT controls. Our results implicate PTP1B in structural plasticity within the hippocampus, likely through modulation of N-cadherin function by ensuring dephosphorylation of ß-catenin on Tyr-654. Disruption of hippocampal PTP1B function or expression leads to elongation of dendritic filopodia and improved learning and memory, demonstrating an exciting novel role for this phosphatase. © 2012 Fuentes et al.

Registro:

Documento: Artículo
Título:Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning
Autor:Fuentes, F.; Zimmer, D.; Atienza, M.; Schottenfeld, J.; Penkala, I.; Bale, T.; Bence, K.K.; Arregui, C.O.
Filiación:Instituto de Investigaciones Biotecnológicas, Universidad de San Martín/CONICET, San Martín, Buenos Aires, Argentina
Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, United States
Max Planck Laboratory of Cellular Dynamics, Faculty of Exact and Natural Sciences, University of Buenos Aires, Ciudad Universitaria. Buenos Aires, Argentina
Palabras clave:beta catenin; nerve cell adhesion molecule; postsynaptic density protein 95; protein tyrosine phosphatase 1B; synapsin I; tyrosine; animal cell; animal experiment; animal tissue; article; brain cortex; cell elongation; cell structure; controlled study; dendritic spine; embryo; enzyme activity; enzyme regulation; filopodium; hippocampus; learning; memory; mouse; nerve cell; nerve cell plasticity; newborn; nonhuman; phenotype; protein analysis; protein deficiency; protein expression; protein function; protein phosphorylation; rat; recall; Animals; beta Catenin; Cadherins; Dendritic Spines; Female; Gene Deletion; Hippocampus; Learning; Memory; Mice; Neuronal Plasticity; Phosphorylation; Pregnancy; Protein Transport; Protein Tyrosine Phosphatase, Non-Receptor Type 1; Rats; Synapses; Tyrosine; Basidiomycota; Mus
Año:2012
Volumen:7
Número:7
DOI: http://dx.doi.org/10.1371/journal.pone.0041536
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CAS:tyrosine, 16870-43-2, 55520-40-6, 60-18-4; Cadherins; Protein Tyrosine Phosphatase, Non-Receptor Type 1, 3.1.3.48; Ptpn1 protein, mouse, 3.1.3.48; Ptpn1 protein, rat, 3.1.3.48; Tyrosine, 55520-40-6; beta Catenin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n7_p_Fuentes

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Citas:

---------- APA ----------
Fuentes, F., Zimmer, D., Atienza, M., Schottenfeld, J., Penkala, I., Bale, T., Bence, K.K.,..., Arregui, C.O. (2012) . Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning. PLoS ONE, 7(7).
http://dx.doi.org/10.1371/journal.pone.0041536
---------- CHICAGO ----------
Fuentes, F., Zimmer, D., Atienza, M., Schottenfeld, J., Penkala, I., Bale, T., et al. "Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning" . PLoS ONE 7, no. 7 (2012).
http://dx.doi.org/10.1371/journal.pone.0041536
---------- MLA ----------
Fuentes, F., Zimmer, D., Atienza, M., Schottenfeld, J., Penkala, I., Bale, T., et al. "Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning" . PLoS ONE, vol. 7, no. 7, 2012.
http://dx.doi.org/10.1371/journal.pone.0041536
---------- VANCOUVER ----------
Fuentes, F., Zimmer, D., Atienza, M., Schottenfeld, J., Penkala, I., Bale, T., et al. Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning. PLoS ONE. 2012;7(7).
http://dx.doi.org/10.1371/journal.pone.0041536