Scolz, M.; Widlund, P.O.; Piazza, S.; Bublik, D.R.; Reber, S.; Peche, L.Y.; Ciani, Y.; Hubner, N.; Isokane, M.; Monte, M.; Ellenberg, J.; Hyman, A.A.; Schneider, C.; Bird, A.W. "GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration" (2012) PLoS ONE. 7(12)
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The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential. © 2012 Scolz et al.


Documento: Artículo
Título:GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration
Autor:Scolz, M.; Widlund, P.O.; Piazza, S.; Bublik, D.R.; Reber, S.; Peche, L.Y.; Ciani, Y.; Hubner, N.; Isokane, M.; Monte, M.; Ellenberg, J.; Hyman, A.A.; Schneider, C.; Bird, A.W.
Filiación:Laboratorio Nazionale The Interuniversity Consortium for Biotechnology, Area Science Park, Trieste, Italy
Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
Department of Molecular Cancer Research, Universitair Medisch Centrum Utrecht, Utrecht, Netherlands
European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Heidelberg, Germany
Department of Medical and Biological Sciences, University of Udine, Udine, Italy
Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, Israel
Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Nijmegen, Netherlands
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Max Planck Institute of Molecular Physiology, Dortmund, Germany
Palabras clave:binding protein; end binding 1 protein; G2 and S phase expressed 1 protein; microtubule associated protein; microtubule plus end tracking protein; microtubule protein; unclassified drug; article; breast cancer; cancer cell culture; cell migration; controlled study; focal adhesion; human; human cell; microtubule; nucleotide sequence; protein function; protein protein interaction; regulatory mechanism; Breast Neoplasms; Cell Line; Cell Movement; DNA Primers; Female; Fluorescent Antibody Technique; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Immunoprecipitation; Kaplan-Meier Estimate; Mass Spectrometry; Microscopy, Fluorescence; Microtubule-Associated Proteins; Microtubules; Neoplasm Invasiveness; Real-Time Polymerase Chain Reaction; RNA Interference; RNA, Small Interfering
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
CAS:DNA Primers; GTSE1 protein, human; MAPRE1 protein, human; Microtubule-Associated Proteins; RNA, Small Interfering


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---------- APA ----------
Scolz, M., Widlund, P.O., Piazza, S., Bublik, D.R., Reber, S., Peche, L.Y., Ciani, Y.,..., Bird, A.W. (2012) . GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration. PLoS ONE, 7(12).
---------- CHICAGO ----------
Scolz, M., Widlund, P.O., Piazza, S., Bublik, D.R., Reber, S., Peche, L.Y., et al. "GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration" . PLoS ONE 7, no. 12 (2012).
---------- MLA ----------
Scolz, M., Widlund, P.O., Piazza, S., Bublik, D.R., Reber, S., Peche, L.Y., et al. "GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration" . PLoS ONE, vol. 7, no. 12, 2012.
---------- VANCOUVER ----------
Scolz, M., Widlund, P.O., Piazza, S., Bublik, D.R., Reber, S., Peche, L.Y., et al. GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration. PLoS ONE. 2012;7(12).