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Abstract:

EGS (external guide sequence) technology is a promising approach to designing new antibiotics. EGSs are short antisense oligoribonucleotides that induce RNase P-mediated cleavage of a target RNA by forming a precursor tRNA-like complex. The ftsZ mRNA secondary structure was modeled and EGSs complementary to two regions with high probability of being suitable targets were designed. In vitro reactions showed that EGSs targeting these regions bound ftsZ mRNA and elicited RNase P-mediated cleavage of ftsZ mRNA. A recombinant plasmid, pEGSb1, coding for an EGS that targets region "b" under the control of the T7 promoter was generated. Upon introduction of this plasmid into Escherichia coli BL21(DE3)(pLysS) the transformant strain formed filaments when expression of the EGS was induced. Concomitantly, E. coli harboring pEGSb1 showed a modest but significant inhibition of growth when synthesis of the EGSb1 was induced. Our results indicate that EGS technology could be a viable strategy to generate new antimicrobials targeting ftsZ. © 2012 Sala et al.

Registro:

Documento: Artículo
Título:Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ
Autor:Sala, C.D.; Soler-Bistué, A.J.C.; Korprapun, L.; Zorreguieta, A.; Tolmasky, M.E.
Filiación:Fundación Instituto Leloir-Instituto de Investigaciones Bioquímicas Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Center for Applied Biotechnology Studies, Department of Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, Fullerton, CA, United States
Unité Plasticité du Génome Bacté rien, Département Génomes et Génétique, Institut Pasteur, Paris, France
Palabras clave:messenger RNA; ribonuclease P; transfer RNA; article; bacterial cell; binding affinity; binding site; complex formation; controlled study; Escherichia coli; experimental design; external guide sequence technology; ftsZ gene; gene; gene targeting; gene technology; growth inhibition; in vitro study; mitosis inhibition; nonhuman; promoter region; protein secondary structure; recombinant plasmid; RNA binding; RNA cleavage; RNA structure; sequence analysis; Anti-Bacterial Agents; Bacterial Proteins; Base Sequence; Cell Division; Cytoskeletal Proteins; Drug Design; Electrophoretic Mobility Shift Assay; Escherichia coli; Microscopy, Confocal; Molecular Sequence Data; Nucleic Acid Conformation; Oligoribonucleotides, Antisense; Promoter Regions, Genetic; Ribonuclease P; RNA Cleavage; Terminator Regions, Genetic
Año:2012
Volumen:7
Número:10
DOI: http://dx.doi.org/10.1371/journal.pone.0047690
Handle:http://hdl.handle.net/20.500.12110/paper_19326203_v7_n10_p_Sala
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CAS:ribonuclease P, 71427-00-4; transfer RNA, 9014-25-9; Anti-Bacterial Agents; Bacterial Proteins; Cytoskeletal Proteins; FtsZ protein, Bacteria; Oligoribonucleotides, Antisense; Ribonuclease P, 3.1.26.5
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_19326203_v7_n10_p_Sala.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v7_n10_p_Sala

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Citas:

---------- APA ----------
Sala, C.D., Soler-Bistué, A.J.C., Korprapun, L., Zorreguieta, A. & Tolmasky, M.E. (2012) . Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ. PLoS ONE, 7(10).
http://dx.doi.org/10.1371/journal.pone.0047690
---------- CHICAGO ----------
Sala, C.D., Soler-Bistué, A.J.C., Korprapun, L., Zorreguieta, A., Tolmasky, M.E. "Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ" . PLoS ONE 7, no. 10 (2012).
http://dx.doi.org/10.1371/journal.pone.0047690
---------- MLA ----------
Sala, C.D., Soler-Bistué, A.J.C., Korprapun, L., Zorreguieta, A., Tolmasky, M.E. "Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ" . PLoS ONE, vol. 7, no. 10, 2012.
http://dx.doi.org/10.1371/journal.pone.0047690
---------- VANCOUVER ----------
Sala, C.D., Soler-Bistué, A.J.C., Korprapun, L., Zorreguieta, A., Tolmasky, M.E. Inhibition of Cell Division Induced by External Guide Sequences (EGS Technology) Targeting ftsZ. PLoS ONE. 2012;7(10).
http://dx.doi.org/10.1371/journal.pone.0047690