Artículo

Croci, D.O.; Cumashi, A.; Ushakova, N.A.; Preobrazhenskaya, M.E.; Piccoli, A.; Totani, L.; Ustyuzhanina, N.E.; Bilan, M.I.; Usov, A.I.; Grachev, A.A.; Morozevich, G.E.; Berman, A.E.; Sanderson, C.J.; Kelly, M.; Gregorio, P.; Rossi, C.; Tinari, N.; Iacobelli, S. (...) Nifantiev, N.E. "Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed" (2011) PLoS ONE. 6(2)
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Abstract:

Sulfated polysaccharides from Laminaria saccharina (new name: Saccharina latissima) brown seaweed show promising activity for the treatment of inflammation, thrombosis, and cancer; yet the molecular mechanisms underlying these properties remain poorly understood. The aim of this work was to characterize, using in vitro and in vivo strategies, the anti-inflammatory, anti-coagulant, anti-angiogenic, and anti-tumor activities of two main sulfated polysaccharide fractions obtained from L. saccharina: a) L.s.-1.0 fraction mainly consisting of O-sulfated mannoglucuronofucans and b) L.s.-1.25 fraction mainly composed of sulfated fucans. Both fractions inhibited leukocyte recruitment in a model of inflammation in rats, although L.s.-1.25 appeared to be more active than L.s.-1.0. Also, these fractions inhibited neutrophil adhesion to platelets under flow. Only fraction L.s.-1.25, but not L.s.-1.0, displayed anticoagulant activity as measured by the activated partial thromboplastin time. Investigation of these fractions in angiogenesis settings revealed that only L.s.-1.25 strongly inhibited fetal bovine serum (FBS) induced in vitro tubulogenesis. This effect correlated with a reduction in plasminogen activator inhibitor-1 (PAI-1) levels in L.s.-1.25-treated endothelial cells. Furthermore, only parent sulfated polysaccharides from L. saccharina (L.s.-P) and its fraction L.s.-1.25 were powerful inhibitors of basic fibroblast growth factor (bFGF) induced pathways. Consistently, the L.s.-1.25 fraction as well as L.s.-P successfully interfered with fibroblast binding to human bFGF. The incorporation of L.s.-P or L.s.-1.25, but not L.s.-1.0 into Matrigel plugs containing melanoma cells induced a significant reduction in hemoglobin content as well in the frequency of tumor-associated blood vessels. Moreover, i.p. administrations of L.s.-1.25, as well as L.s.-P, but not L.s.-1.0, resulted in a significant reduction of tumor growth when inoculated into syngeneic mice. Finally, L.s.-1.25 markedly inhibited breast cancer cell adhesion to human platelet-coated surfaces. Thus, sulfated fucans are mainly responsible for the anti-inflammatory, anticoagulant, antiangiogenic, and antitumor activities of sulfated polysaccharides from L. saccharina brown seaweed. © 2011 Croci et al.

Registro:

Documento: Artículo
Título:Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed
Autor:Croci, D.O.; Cumashi, A.; Ushakova, N.A.; Preobrazhenskaya, M.E.; Piccoli, A.; Totani, L.; Ustyuzhanina, N.E.; Bilan, M.I.; Usov, A.I.; Grachev, A.A.; Morozevich, G.E.; Berman, A.E.; Sanderson, C.J.; Kelly, M.; Gregorio, P.; Rossi, C.; Tinari, N.; Iacobelli, S.; Rabinovich, G.A.; Nifantiev, N.E.
Filiación:Laboratorio de Inmunopatología, IBYME, CONICET y Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina
Department of Oncology and Neurosciences, University G. D'Annunzio Medical School and Foundation, Chieti, Italy
V.N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, Russian Federation
Consorzio Mario Negri Sud, Laboratory of Vascular Biology and Pharmacology, Santa Maria Imbaro, Chieti, Italy
Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Laboratory of Plant Polysaccharides, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Scottish Association for Marine Sciences, Oban, Argyll, Scotland, United Kingdom
S.S. Annunziata Hospital, School of Medicine, University G. D'Annunzio, Chieti, Italy
Palabras clave:bovine serum albumin; carbohydrate derivative; fibroblast growth factor; hemoglobin; o sulfated mannoglucuronofucan derivative; plasminogen activator inhibitor 1; polysaccharide sulfate; sulfated fucan derivative; unclassified drug; angiogenesis inhibitor; anticoagulant agent; antiinflammatory agent; biological product; fucoidin; fucose; polysaccharide; angiogenesis; animal experiment; animal model; antiangiogenic activity; anticoagulation; antiinflammatory activity; antineoplastic activity; article; biological activity; breast cancer; cancer growth; cancer inhibition; cell level; cell surface; controlled study; drug determination; drug efficacy; drug isolation; drug structure; endothelium cell; fibroblast; human; human cell; in vitro study; in vivo study; Laminaria; Laminaria saccharina; leukocyte migration inhibition; melanoma; melanoma cell; mouse; nonhuman; partial thromboplastin time; peritonitis; rat; thrombocyte adhesion; tumor vascularization; animal; brown alga; C57BL mouse; cell culture; chemistry; drug effect; evaluation; female; inflammation; Laminaria; metabolism; pathology; physiology; seaweed; Wistar rat; Bovinae; Mus; Rattus; Saccharina latissima; Angiogenesis Inhibitors; Animals; Anti-Inflammatory Agents; Anticoagulants; Biological Products; Cells, Cultured; Endothelial Cells; Female; Fucose; Humans; Inflammation; Laminaria; Mice; Mice, Inbred C57BL; Neovascularization, Physiologic; Phaeophyta; Polysaccharides; Rats; Rats, Wistar; Seaweed
Año:2011
Volumen:6
Número:2
DOI: http://dx.doi.org/10.1371/journal.pone.0017283
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CAS:fibroblast growth factor, 62031-54-3; hemoglobin, 9008-02-0; plasminogen activator inhibitor 1, 140208-23-7; polysaccharide sulfate, 79933-28-1; fucoidin, 9072-19-9; fucose, 3615-37-0, 3713-31-3; Angiogenesis Inhibitors; Anti-Inflammatory Agents; Anticoagulants; Biological Products; Fucose, 3713-31-3; Polysaccharides; fucoidan, 9072-19-9
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_19326203_v6_n2_p_Croci.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v6_n2_p_Croci

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Citas:

---------- APA ----------
Croci, D.O., Cumashi, A., Ushakova, N.A., Preobrazhenskaya, M.E., Piccoli, A., Totani, L., Ustyuzhanina, N.E.,..., Nifantiev, N.E. (2011) . Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed. PLoS ONE, 6(2).
http://dx.doi.org/10.1371/journal.pone.0017283
---------- CHICAGO ----------
Croci, D.O., Cumashi, A., Ushakova, N.A., Preobrazhenskaya, M.E., Piccoli, A., Totani, L., et al. "Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed" . PLoS ONE 6, no. 2 (2011).
http://dx.doi.org/10.1371/journal.pone.0017283
---------- MLA ----------
Croci, D.O., Cumashi, A., Ushakova, N.A., Preobrazhenskaya, M.E., Piccoli, A., Totani, L., et al. "Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed" . PLoS ONE, vol. 6, no. 2, 2011.
http://dx.doi.org/10.1371/journal.pone.0017283
---------- VANCOUVER ----------
Croci, D.O., Cumashi, A., Ushakova, N.A., Preobrazhenskaya, M.E., Piccoli, A., Totani, L., et al. Fucans, but not fucomannoglucuronans, determine the biological activities of sulfated polysaccharides from laminaria saccharina brown seaweed. PLoS ONE. 2011;6(2).
http://dx.doi.org/10.1371/journal.pone.0017283